Anti mouse nk1.1 antibody

CD169-DTR heterozygous or wild-type (WT) male mice were intraperitoneally injected with 40 ng/g of diphtheria toxin (DT) (List Labs, Campbell, CA, USA) diluted in 200 μl DPBS at early stages (a day before and 4 days after tumor injection) and intermediate stages (5 and 10 days after tumor injection) of tumor progression.
For NK cell depletion, C57BL/6 J male mice were intraperitoneally injected with 500 μg of anti-mouse NK1.1 antibody (Bioxcell, Lebanon, NH, USA) diluted in 200 μl DPBS. Mouse IgG2a (Bioxcell) was used as an isotype control. Injections were performed 1 and 2 days before and 7 days after tumor injection.
To interfere with monocyte migration, C57BL/6 J male mice were intraperitoneally injected with 5 mg/kg of CCR2 inhibitor RS102895 (Sigma–Aldrich) diluted in 200 μl DPBS starting one day before tumor injection and repeated every 3 days.