Restriction endonuclease enzymes

4-Aminoantipyrine and Isopropyl ß-D-1-thiogalactopyranoside (IPTG) from Hi-Media, India; Catechol (extra pure, 99%) from SRL, India and ccMA from Sigma-Aldrich, India were purchased. Restriction endonuclease enzymes and T₄ DNA Ligase enzyme were purchased from NEB, United States. pGEM-T Easy Vector Systems (Promega, United States) was used for TA cloning. E. coli DH5α, E. coli BL21 and pET30b(+) vector were procured from the original stock of our lab. Ni-NTA Agarose resin from Qiagen, Germany; Sephacryl S-300 HR resin from Cytiva, United States and NativeMark^™ Unstained Protein Standards were obtained from Invitrogen, United States.

(S)-Reticuline was a gift from Peter Facchini (University of Calgary). (R,S)-Norlaudanosoline was purchased from Enamine Ltd. (Kiev, Ukraine), (R)-reticuline, salutaridine, thebaine, oripavine, codeine and morphine were from TRC Inc. (North York, Ontario, Canada). Antibiotics, growth media and α-D-glucose were purchased from Sigma-Aldrich. Restriction endonuclease enzymes were from New England Biolabs (NEB). Yeast genomic DNA used as template for PCR was purified using the DNeasy Blood and Tissue Kit (Qiagen). Polymerase chain reactions (PCRs) were performed using Phusion High-Fidelity DNA polymerase (NEB/Thermo Scientific). PCR-amplified products were gel purified using the QIAquick Purification Kit (Qiagen). Plasmid extractions were done using the GeneJET plasmid mini-prep kit (Thermo Scientific). HPLC-grade water was purchased from Fluka. HPLC-grade acetonitrile was purchased from Fischer Scientific.