All siRNA duplexes used in this study were purchased from Invitrogen or Sigma. Transfection and co-transfection was carried out using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions. Cells were harvested 72 h after transfection. The individual siRNA duplexes used were: siFANCD2 (5′CAGAGUUUGCUUCACUCUCUATT-3′) (Invitrogen) (4 (link)) siFANCA (5′AAGGGUCAAGAGGGAAAAAUA-3′) (Invitrogen) (5 (link)); Luciferase Control (Ctrl) (5′UCGAAGUAUUCCGCGUACGTT-3′) (Invitrogen) (8 (link)).
Sifancd2
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SiFANCD2 is a lab equipment product designed for use in scientific research. It serves as a core functional component, but a detailed description cannot be provided while maintaining an unbiased and factual approach. The specific intended use and details of the product's capabilities are not available.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)
Si nc, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Colivelin, by Santa Cruz Biotechnology (1 mentions)
Fer 1, by Selleck Chemicals (1 mentions)
Erastin, by Selleck Chemicals (1 mentions)
Si nc, by RiboBio (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
3 protocols using sifancd2
1
siRNA Transfection for FANC Knockdown
2
Osteosarcoma Cell Manipulation and Ferroptosis Regulation
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The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO2. siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China). According to the instructions of manufacturer, si-FANCD2 or si-NC were transfected with osteosarcoma cells using Lipofectamine 3000 reagent (Invitrogen) at 37 °C for 48 h. For ferroptosis induction or inhibition, U2OS cells (1 × 106 cells) were further treated with Erastin (10 µΜ, Selleck, Houston, TX, USA) or Fer-1(60 nM, Selleck) for 24 h, respectively. For JAK2/STAT3 signaling pathway activation, cells were treated with colivelin (1 nM, Santa Cruz, Dallas, TX, USA) for 24 h.
3
Investigating Cellular Pathways with siRNA Knockdown
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All siRNA duplexes used were purchased from Invitrogen or Sigma. Transfection and cotransfection were carried out using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions. Cells were harvested 72 h after transfection. The individual siRNA duplexes used were: siFANCD2 (5 ′ -CAGAGUUUGCUUCACUCUCUATT-3 ′ ) (Invitrogen) [8] ; sihnRNP U (5'-GGCCGUGGUAGUUACUCAATT-3 ′ ) (Invitrogen) [53] ; siDDX47 (5'-CUCCAUGCUCCCUUAACUCCATT-3 ′ ) (Invitrogen) [54] ; siLuciferase Control (siLuc) (5 ′ -UCGAAGUAUUCCGCGUA CGTT-3 ′ ) (Invitrogen) [55] . Plasmid transfection was done using lipofectamine 3000 (Invitrogen) according to the manufacturer's instructions.
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