The 36PillarPlate with HLOs was sandwiched with an opaque white 384-well plate containing 20 μL of CellTiter-Glo® Reagent from CellTiter-Glo® cell viability kit (Promega, Madison, WI, USA) and 20 μL of cell culture medium in each well to measure cellular adenosine triphosphate (ATP) levels. To induce cell lysis, the sandwiched pillar/well plates were placed on an orbital shaker for 25 minutes. After stabilizing the luminescence for 15 minutes at room temperature, the luminescence signals were recorded using the microtiter well plate reader (Synergy H1) at an emission wavelength of 560 nm. A standard calibration curve was generated using ATP disodium salt (Sigma Aldrich) at a concentration range of 10 nM to 1 μM.
Celltiter glo reagent from celltiter glo cell viability kit
Manufactured by Promega
Sourced in United States
The CellTiter-Glo® Reagent is a component of the CellTiter-Glo® cell viability kit. It is a luminescent assay that quantifies the amount of ATP present in a sample, which is directly proportional to the number of viable cells.
Automatically generated - may contain errors
2 protocols using celltiter glo reagent from celltiter glo cell viability kit
1
Quantification of Cellular ATP Levels
2
Cell Viability Measurement using CellTiter-Glo
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Example 16
Measurement of cell viability using CellTiter-Glo® luminescent cell viability assay kit: The pillar plates with cells were sandwiched with an opaque white 384-well plate containing 20 μL of CellTiter-Glo® Reagent from CellTiter-Glo® cell viability kit (Promega, Madison, WI, USA) and 20 μL of cell culture medium in each well to measure cellular adenosine triphosphate (ATP) levels. To induce cell lysis, the sandwiched pillar/well plates were placed on an orbital shaker for 25 minutes. After stabilizing the luminescence for 15 minutes at room temperature, the luminescent signals were recorded using the microtiter well plate reader (Synergy H1) at an emission wavelength of 560 nm. A standard calibration curve was generated using ATP disodium salt (Sigma Aldrich) at a concentration range of 10 nM to 1 μM.
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