Tissue culture polystyrene petri dishes

Manufactured by Greiner

Sourced in Austria

Tissue culture polystyrene Petri dishes are a type of laboratory equipment used for the cultivation and maintenance of cells, tissues, and microorganisms. These dishes provide a sterile, stable, and uniform surface for cell growth and experimentation. The dishes are made of high-quality polystyrene material and are designed to meet the specific requirements of cell culture applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Biowest (2 mentions) L glutamine, by Merck Group (2 mentions) Amphotericin b, by Merck Group (2 mentions) Edta solution, by Merck Group (1 mentions) Trypsin, by Merck Group (1 mentions) Penicillin, by Merck Group (1 mentions) Hanks balanced salt solution (hbss), by Merck Group (1 mentions) Streptomycin solution, by Merck Group (1 mentions) Gentamycin, by Merck Group (1 mentions)

Spelling variants of this product

Petri dishes (33 citations) Petri dish (19 citations) Tissue culture dishes (6 citations) Polystyrene petri dishes (4 citations)

3 protocols using tissue culture polystyrene petri dishes

HeLa Cell Culture Protocol

Cited 3 times

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HeLa cells
(ECACC 93021013 human, cervix, epitheloid, carcinoma) were routinely
cultured in tissue culture polystyrene Petri dishes (Greiner Bio-One
International GmbH, Kremsmünster, Austria) placed in a humidified
incubator (37 °C, 5% CO₂). The cells were maintained
in Dulbecco’s modified Eagle’s medium (DMEM), supplemented
with 10% fetal bovine serum (FBS, Biowest SAS, France), 4 mM l-glutamine, 0.25 μg/mL amphotericin B, 100 U/mL penicillin,
and 100 μg/mL streptomycin solution. On reaching 80% confluence,
the cells were detached every 3–4 days using 0.05% (w/v) trypsin
and 0.02% (w/v) ethylenediaminetetraacetic acid (EDTA) solution and
not used beyond passage 20.^{6 (link),38 (link)} The cell adhesion assay
buffer was prepared by adding 20 mM HEPES to HBSS and adjusted to
pH 7.0 with a 1 mM NaOH.^{5 (link),30 (link)}

Peter B., Ungai-Salanki R., Szabó B., Nagy A.G., Szekacs I., Bősze S, & Horvath R. (2018). High-Resolution Adhesion Kinetics of EGCG-Exposed Tumor Cells on Biomimetic Interfaces: Comparative Monitoring of Cell Viability Using Label-Free Biosensor and Classic End-Point Assays. ACS Omega, 3(4), 3882-3891.

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HeLa Cell Culture and Adhesion Assay Protocol

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HeLa cells were routinely cultured in tissue culture polystyrene Petri dishes (Greiner Bio-One International GmbH, Kremsmünster, Austria) placed in a humidified incubator (37 °C, 5% CO₂). The cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (Biowest SAS, France), 4 mM L-glutamine (from Sigma-Aldrich Chemie GmbH, Munich, Germany), 0.25 μg∕ml amphotericin B (from Sigma-Aldrich Chemie GmbH, Munich, Germany), 100 U∕ml penicillin (from Sigma-Aldrich Chemie GmbH, Munich, Germany) and 100 μg∕ml streptomycin solution (from Sigma-Aldrich Chemie GmbH, Munich, Germany). On reaching 80% confluence, cells were detached every 3–5 days using 0.05% (w/v) trypsin (from Sigma-Aldrich Chemie GmbH, Munich, Germany), 0.02% (w/v) EDTA solution (from Sigma-Aldrich Chemie GmbH, Munich, Germany) and were not used beyond passage 2047 . Cell adhesion assay buffer was prepared by adding 20 mM HEPES to Hank’s balanced salt solution (HBSS, from Sigma-Aldrich Chemie GmbH, Munich, Germany) and adjusted to pH 7.0 with 1 mM NaOH (hereafter HBSS-HEPES).

Peter B., Farkas E., Forgacs E., Saftics A., Kovacs B., Kurunczi S., Szekacs I., Csampai A., Bosze S, & Horvath R. (2017). Green tea polyphenol tailors cell adhesivity of RGD displaying surfaces: multicomponent models monitored optically. Scientific Reports, 7, 42220.

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Routine Culture of HeLa and HEK293 Cells

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HeLa and HEK293 cells were routinely cultured in tissue culture polystyrene Petri dishes (Greiner) placed in a humidified incubator (37°C, 5% CO₂). Culture medium was prepared by supplementing Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 4 mM L-glutamine, 40 µg/ml gentamycin, and 0.25 µg/ml amphotericin B (all these substances were purchased from Sigma-Aldrich).

Orgovan N., Peter B., Bősze S., Ramsden J.J., Szabó B, & Horvath R. (2014). Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor. Scientific Reports, 4, 4034.

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