Sc 10800

Manufactured by Santa Cruz Biotechnology

The Sc-10800 is a laboratory centrifuge designed for general-purpose applications. It features a variable speed control, a digital display, and a rotor capacity of up to 6 x 50 mL tubes. The centrifuge is capable of speeds up to 6,000 rpm and can generate a maximum relative centrifugal force of 4,070 x g. The Sc-10800 is a durable and reliable piece of equipment for basic sample separation and preparation tasks in a research or clinical laboratory setting.

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Lab products found in correlation

H 300, by Santa Cruz Biotechnology (1 mentions) Mouse anti ha h9658, by Merck Group (1 mentions) Rabbit anti pkcα, by Cell Signaling Technology (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) Rat anti ha, by Roche (1 mentions) L 21409, by Thermo Fisher Scientific (1 mentions) Ab15282, by Merck Group (1 mentions) Mab3073, by Merck Group (1 mentions)

2 protocols using sc 10800

Protein Interaction and Modification Analysis

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Flag-SUMO1, RGS-SENP1 and RGS-SENP1m were gifts from Jinke Cheng (Shanghai Jiao Tong University School of Medicine, Shanghai, China). HA-PKCα was purchased from Addgene. The following primary antibodies and dilutions were used: Mouse Anti-RGS (34610, 0.1 μg ml⁻¹ dilution) was from Qiagen. Mouse Anti-Flag (M2, F3165, 1:1,000), Mouse Anti-PKC (P5704, 1:500), Mouse Anti-HA (H9658, 1:2,000) and Anti-Flag M2 affinity gel were from Sigma. Rabbit Anti-SUMO1 (4940, 1:1,000), Rabbit Anti-MARCKS (1:1,000), Rabbit Anti-p-MARCKS (1:1,000) and Rabbit Anti-PKCα (1:1,000) were purchased from Cell Signaling. Rat Anti-HA (1:2,000) was purchased from Roche. Anti-HA agarose was from Pierce Biotechnology. Anti-GlyR was from Abcam (rabbit, 1:300) or Synaptic Systems (mouse, 1:300). Anti-PKC antibodies (sc-17804, A9, mouse, 1:500; sc-10800, H-300, rabbit 1:500) were from Santa Cruz Biotechnology.

Sun H., Lu L., Zuo Y., Wang Y., Jiao Y., Zeng W.Z., Huang C., Zhu M.X., Zamponi G.W., Zhou T., Xu T.L., Cheng J, & Li Y. (2014). Kainate receptor activation induces glycine receptor endocytosis through PKC deSUMOylation. Nature Communications, 5, 4980.

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Retinal Neurotransmitter Receptor Localization

Cited 1 time

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The following antibody and lectins were utilized in this study: Rabbit anti-cone arrestin (cones; Millipore; AB15282; 1:1000: RRID:AB_1163387), mouse (IgG1) anti-calsenilin (type 4 cone bipolar cells; Millipore; 05–756; 1:500: RRID:AB_309969), mouse (IgG1) anti-Goa (ON bipolar cells; Millipore; MAB3073; 1:1000: RRID:AB_94671), Peanut agglutinin (PNA) (cone pedicles; Molecular Probes; L21409; 1:1000: RRID:AB_2315178), rabbit anti-PkaRIIβ (type 3b bipolar cells; BD biosciences 610625; 1:500: RRID:AB_397957), rabbit anti-PKCα (rod bipolar cells; Santa Cruz Biotechnology; sc-10800; 1:500: RRID:AB_2168560). DAPI was included in the second post-secondary antibody wash at a dilution of 1:50,000 of a 1 mg/ml stock (RRID:AB_2307445). Secondary Antibodies: Secondary antibodies were acquired from Jackson Immuno-Research (RRID:SCR_010488) and used at a concentration of 1:1000. The specificity of antibody reagents used in this study have previously been experimentally verified and described in detail (de Andrade et al., 2014 (link)).

Simmons A.B., Camerino M.J., Clemons M.R., Sukeena J.M., Bloomsburg S., Borghuis B.G, & Fuerst P.G. (2019). Increased density and age-related sharing of synapses at the cone to OFF bipolar cell synapse in the mouse retina. The Journal of comparative neurology, 528(7), 1140-1156.

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