Sml1524

Manufactured by Merck Group

The SML1524 is a laboratory equipment product manufactured by Merck Group. It serves as a general-purpose device for handling and processing samples in a laboratory setting. The core function of the SML1524 is to facilitate standard laboratory procedures, but a detailed description of its intended use is not available.

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Lab products found in correlation

Ham s f12 medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Basic human fgf, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions) Gsk2801, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) D1515, by Merck Group (1 mentions) A8380, by Merck Group (1 mentions) Gfp ax10 cam 105, by Zeiss (1 mentions)

Spelling variants of this product

JQ1 (SML1524) (4 citations)

5 protocols using sml1524

Prostate Cancer Spheroid Culture and Drug Treatment

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All the indicated cell lines were purchased from the American Type Culture Collection. PC3 cells were cultured in RPMI 1640 medium and Ham's F12 medium (1:1; Gibco) containing 10% FBS (Gibco) and 1% penicillin–streptomycin (Gibco). DU‐145 were cultured in RPMI 1640 medium containing 10% FBS and 1% penicillin–streptomycin. PCa spheres were cultured in serum‐free DMEM/F12 medium (1:1, Gibco) supplemented with 20 ng/ml of basic human FGF (Sigma), 20 ng/ml of EGF (Sigma), 3 μg/ml of Insulin (Sigma), and 1× B27 (Gibco). 1 × 10⁴ cells were seeded into low adhesion petri dishes (Greiner) and cultured for 6–8 days. About 20% of media was changed every 2 days. All cells were regularly tested for mycoplasma contamination. PC3 cells were treated with 1 μM of BAZ2A‐BRD inhibitors BAZ2‐ICR (SML1276, Sigma) and GSK2801 (SML0768, Sigma), EZH2 inhibitor GSK126 (S7061‐5MG, Lubio), BET inhibitors JQ1 (SML1524, Sigma), or DMSO as control. Fresh medium supplemented with the drugs was added every 2 days. PCa spheres were culture for 6 days. Cells were collected and transferred to a 24‐well plate to image and quantify the number of PCa spheres upon drug treatments.

Peña‐Hernández R., Aprigliano R., Carina Frommel S., Pietrzak K., Steiger S., Roganowicz M., Lerra L., Bizzarro J, & Santoro R. (2021). BAZ2A‐mediated repression via H3K14ac‐marked enhancers promotes prostate cancer stem cells. EMBO Reports, 22(11), e53014.

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JQ1 Treatment Protocol for Cell Lines

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Cells were then treated for 6 hours with 500nM JQ1 in DMSO unless otherwise indicated (Sigma-Aldrich SML1524) or an equivalent volume of DMSO.

Hung K.L., Yost K.E., Xie L., Shi Q., Helmsauer K., Luebeck J., Schöpflin R., Lange J.T., Chamorro R., Weiser N.E., Chen C., Valieva M.E., Wong I.T., Wu S., Dehkordi S.R., Duffy C.V., Kraft K., Tang J., Belk J.A., Rose J.C., Corces M.R., Granja J.M., Li R., Rajkumar U., Friedlein J., Bagchi A., Satpathy A.T., Tjian R., Mundlos S., Bafna V., Henssen A.G., Mischel P.S., Liu Z, & Chang H.Y. (2021). ecDNA hubs drive cooperative intermolecular oncogene expression. Nature, 600(7890), 731-736.

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Bromodomain Inhibitor Impacts on Algal Antheridia

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The thallus fragments carrying antheridia were treated for 24 h and 48 h with a bromodomain inhibitor, (+)-JQ1 (hereafter referred to as JQ1) (Sigma, SML1524) at 100 μM concentration. The solution of the inhibitor was prepared with the water from the natural environment, with DMSO (Sigma) in the ratio 20 mg/mL. These duration times of treatment with the bromodomain inhibitor were used, because previous analyses [3 (link),28 (link)] were also based on the same scheme. In the studies on animal material, using cell lines, a broad concentration range of bromodomain inhibitors, but lower than in this alga, was used. Because in the case of C. vulgaris, antheridial filaments were developing inside the multicellular antheridium, the concentration of the inhibitor used was many times higher in comparison with the cell line model. Initially the concentration of 50 μM was used, however, barely noticeable changes appeared only in single spermatids.

, & Wojtczak A. (2020). Blocking the Bromodomains Function Contributes to Disturbances in Alga Chara vulgaris Spermatids Differentiation. Cells, 9(6), 1352.

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Targeted Heart Failure Drug Assay

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For targeted heart failure drug testing, 10 nM of JQ1 (SML1524, Sigma) was added to the media on D0 every 2 days for infarct organoids for the length of the experiment (10 days). For 2D hiPSC-CM experiments, cells were plated for 2 days on gelatin-coated 96 well plates (20,000 cells per well) in iCell Cardiomyocytes Maintenance Medium (CDI), changed to organoid media for 2 days in control or “2D infarction” conditions (1% O₂ with 1 μM NE), and then doxorubicin doses were added to culture for 2 more days. For detection of exacerbation of drug-induced cardiotoxicity in cardiac organoids, a range (0-50 μM) of doxorubicin (D1515, Sigma) was added to media on D10 and cultured for 2 days in control or infarct conditions.

Richards D.J., Li Y., Kerr C.M., Yao J., Beeson G.C., Coyle R.C., Chen X., Jia J., Damon B., Wilson R., Hazard E.S., Hardiman G., Menick D., Beeson C.C., Yao H., Ye T, & Mei Y. (2020). Human cardiac organoids for the modelling of myocardial infarction and drug cardiotoxicity. Nature biomedical engineering, 4(4), 446-462.

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Prostate Sphere-Formation Assay Protocol

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Prostate sphere-formation assays were performed following a previously described protocol^{54 (link)}. Briefly, 1 × 10⁴OLFM4-wild or OLFM4-knockout GFP reporter RWPE1 cells were suspended in 50 µl growth medium and mixed with 50 µl Matrigel, then cultured in 12-well plates for up to 14 days. For cells treated with 100 nM DHT (Sigma-Aldrich, #A8380) or 0.1 to 1 μM (+)-JQ1 (Sigma-Aldrich, #SML1524), the treatment medium was replaced with fresh medium containing the treatment reagent every 2 days. Dimethyl sulfoxide (DMSO) was used as a vehicle control for all treatment reagents. Images of spheres were captured with an AX10 cam 503 mono or GFP AX10 Cam 105 Color with a ZEISS microscope (AX 10) and ZEISS software for different timepoints, and GFP-positive colonies larger than 50 µm in diameter were counted.

Li H., Chaitankar V., Zhu J., Chin K., Liu W., Pirooznia M, & Rodgers G.P. (2020). Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC. Scientific Reports, 10, 21924.

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