Zombie live dead dye

Manufactured by BioLegend

Zombie Live Dead dye is a fluorescent cell viability dye used for flow cytometry analysis. It enables the detection of live and dead cells in a sample.

Automatically generated - may contain errors

Lab products found in correlation

Cd107a alexafluor647, by BioLegend (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Ma900, by Sony (1 mentions) Verse system, by BD (1 mentions)

2 protocols using zombie live dead dye

T Cell Degranulation and Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

To detect degranulation of T cells, CD107a‐AlexaFluor647 (BioLegend, San Diego, CA) was added at the start of coculture and proliferation was detected by labeling PBMCs with CellTrace Violet (Invitrogen). T Cell subsets were gated using Zombie Live Dead dye (BioLegend) and the antibodies detailed in Supporting Table S1. Data were acquired on a Sony MA900 (Sony Biotechnology, San Jose, CA) and analyzed by FlowJo (v10; TreeStar Inc., Ashland, OR).

Fergusson J.R., Wallace Z., Connolly M.M., Woon A.P., Suckling R.J., Hine D.W., Barber C., Bunjobpol W., Choi B., Crespillo S., Dembek M., Dieckmann N., Donoso J., Godinho L.F., Grant T., Howe D., McCully M.L., Perot C., Sarkar A., Seifert F.U., Singh P.K., Stegmann K.A., Turner B., Verma A., Walker A., Leonard S., Maini M.K., Wiederhold K., Dorrell L., Simmons R, & Knox A. (2020). Immune‐Mobilizing Monoclonal T Cell Receptors Mediate Specific and Rapid Elimination of Hepatitis B–Infected Cells. Hepatology (Baltimore, Md.), 72(5), 1528-1540.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry for Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspensions from spleens, lymph nodes and tumor tissues were prepared. All cells were first stained with Zombie Live/Dead dye (BioLegend) at 4 °C for 30 min. To analyze surface markers, cells were stained with antibodies at 4 °C for 30 min. For intracellular cytokine staining, cells were fixed, permeabilized and labeled with intracellular cytokine antibodies. Antibody information was described in Supplementary Table 2. Data were acquired using Verse system (BD) and analyzed with FlowJo (v.10.5.3).

Ma J., Tang L., Tan Y., Xiao J., Wei K., Zhang X., Ma Y., Tong S., Chen J., Zhou N., Yang L., Lei Z., Li Y., Lv J., Liu J., Zhang H., Tang K., Zhang Y, & Huang B. (2024). Lithium carbonate revitalizes tumor-reactive CD8+ T cells by shunting lactic acid into mitochondria. Nature Immunology, 25(3), 552-561.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!