Vglut gal4

Manufactured by Bloomington Drosophila Stock Center

Sourced in United States

The VGlut-Gal4 is a genetic tool used in Drosophila research. It is a Gal4 driver line that expresses the Gal4 transcription factor under the control of the vesicular glutamate transporter (VGlut) promoter, which drives expression in glutamatergic neurons.

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6 protocols using vglut gal4

Drosophila Neurogenetic Toolkit Protocols

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Flies were kept on a standard corn meal, corn syrup, yeast, and agar medium at room temperature. UAS-mCD8-ChRFP (#27392), vGlut-Gal4 (#26160), and Gad1-Gal4 (#47140) were newly obtained from the Bloomington Drosophila Stock Center (Indiana, USA) for these experiments. The UAS-Ca-alpha1T-IR line (#48008) was obtained from the Vienna Drosophila RNAi Center. EP line G1047 was obtained from Genexel. c465-Gal4 and 210y-Gal4 were gifts from J. Douglas Armstrong28 (link). The following stocks were all described previously: elav-Gal429 (link), elav-GS-Gal419 (link), Cha-Gal430 (link), 104y-Gal431 (link), c309-Gal432 (link), MB247-Gal433 (link), pdf-Gal434 (link), TH-Gal435 (link), GMR-Gal436 (link), clk8.0-Gal437 (link), dilp2-Gal438 (link), Tdc2-Gal4 and TRH-Gal439 (link), c161-Gal4 and c232-Gal440 (link), and c929-Gal4 and 386Y-Gal441 (link).

Jeong K., Lee S., Seo H., Oh Y., Jang D., Choe J., Kim D., Lee J.H, & Jones W.D. (2015). Ca-α1T, a fly T-type Ca2+ channel, negatively modulates sleep. Scientific Reports, 5, 17893.

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Drosophila Neuronal Genetic Toolkit

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All flies were raised on standard food at 25°C with a 12-hour light:12-hour dark cycle, except for the Tubulin-Gal80^ts experiments to induce expression at different developmental stages, where flies were raised at either 18° or 29°C. Male and female flies were collected at eclosion and aged as specified before performing experiments. VT030559-GAL4 was obtained from the Vienna Drosophila Resource Center stock center. VAChT^[MI08244] (#55439), nsyb-GAL4 (#51941), VGluT-GAL4 (#60312), VGluT-p65-AD (#82986), VGluT-GAL4-DBD (#60313), ChAT-GAL4 (#60317), GH146-GAL4 (#30026), GMR81C04-GAL4 (#48378), VGAT-GAL4 (#84696), UAS-miR-190-sponge (#61397), UAS-scramble-sponge (#61501), UAS-Flp (#4539), UAS-CD4-GFP^1–10 (#93016), VGluT-Gal80 (#58448), and tubulin-Gal80^ts (#7016) were obtained from Bloomington Drosophila Stock Center. UAS-myrGFP-2A–RedStinger (27 (link)) was obtained from the Ganetzky laboratory at University of Wisconsin, and UAS-UNC84::GFP was from G. Henry at Janelia Research Campus. Table S1 lists all the lines used in this study with their source and whether their mRNA contains the native gene’s 3′UTR. Table S2 lists the full genotypes of all lines used in this study by figure panel.

Chen N., Zhang Y., Rivera-Rodriguez E.J., Yu A.D., Hobin M., Rosbash M, & Griffith L.C. (2023). Widespread posttranscriptional regulation of cotransmission. Science Advances, 9(22), eadg9836.

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Drosophila melanogaster Genetic Toolkit

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Drosophila melanogaster was cultured on a standard medium at 22–25°C. Detailed genotypes and sexes used for experiments are described in Table S1. Stocks used in this study include: Vglut-Gal4 (Bloomington Drosophila Stock Center (BDSC #: 24635), BG57-Gal4 (gift from Vivian Budnik), atl² (gift from James McNew), third chromosome deficiency (BDSC #: 7948), UAS-BiP:sfGFP:HDEL (BDSC #: 24635), 20XUAS-tdTomato:Sec61β (BDSC #: 64746), UAS-CD8:RFP (BDSC #: 27391) and UAS-GFP (gift from Griffith lab).

Quiñones-Frías M.C., Ocken D.M, & Rodal A. (2023). High-resolution imaging of presynaptic ER networks in Atlastin mutants. bioRxiv.

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Genetic Tools for Drosophila Neuroscience

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All strains developed for this study are listed in Supplementary Table 2. Aop-CD4:spGFP11 and UAS-CD4:spGFP1–10 were gifts of K. Scott. Drivers GH146-Gal4, GH146-LexA, GH146-QF, Orco-Gal4, Orco-LexA, Or92a-Gal4, GR21a-Gal4, VGlut-Gal4, MB247-Gal4 and MB247-LexA are available from the Bloomington Stock Center. IR84a-LexA was a gift from R. Benton. panR8-Gal4 (ref. 38 (link); a combination of rh5-Gal4+rh6-Gal4) and Tm5c-LexA10 (link) have been previously published, as have Ir93a-LexA9 (link) and VT40053-Gal4 (ref. 9 (link)). Flies used for Fig. 1 came from regular laboratory fly vials, were not sexed and were 2–6 days old. Flies used in Fig. 2 were all females (due to constraints of the genetic cross), and 6–7 days old—this is because the tdTomato signal was difficult to detect in younger flies.

Macpherson L.J., Zaharieva E.E., Kearney P.J., Alpert M.H., Lin T.Y., Turan Z., Lee C.H, & Gallio M. (2015). Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation. Nature Communications, 6, 10024.

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Drosophila Neuronal Lineage Tracing

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The following strains were used: Janelia Gal4 lines 24G08 Gal4 (Bloomington stock 49316) and 31B08 Gal4 (Bloomington stock 49351), Tdc2 Gal4 (Bloomington stock 9313), VGlut Gal4 (Bloomington stock 26160), VGlut MiMIC RMCE line with EGFP reporter (and GFDTF tagged) (Nagarkar-Jaiswal et al., 2015 (link)) (Bloomington stock 59411), UAS mGFP (Bloomington stocks 35839), UAS reaper (Bloomington stock 5824), UAS TeTxLC (Bloomington stock 28997). All crosses were raised at 25°C. The white Canton S (w^CS) strain (outcrossed to CS, from Jean-Maurice Dura, IGH Montpellier, France) was used as wild type control and was crossed with the different Gal4 lines for control experiments.

Pons M., Soulard C., Soustelle L., Parmentier M.L., Grau Y, & Layalle S. (2017). A New Behavioral Test and Associated Genetic Tools Highlight the Function of Ventral Abdominal Muscles in Adult Drosophila. Frontiers in Cellular Neuroscience, 11, 371.

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Diverse Drosophila Transgenic Lines

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The following lines were obtained from the Bloomington Drosophila Stock Center: nSyb-GAL4 (#51635), vGlut-GAL4 (#26160), ChAT-GAL4 (#6793), vGAT-GAL4 (#58409), Trh-GAL4 (#38389), Tdc2-GAL4 (#9313), OK107-GAL4 (#854), Akh-GAL4 (#25683), Mip-GAL4 (#51983), Ilp2-GAL4 (#37516), Eth-GAL4 (#51982), Fmrfa-GAL4 (#56837), Lk-GAL4 (#51993), NPF-GAL4 (#25681), phm-GAL4 (#80577), ple-GAL4 (#8848), SifAR-GAL4 (#76670), Tk-GAL4 (#51973), Eip93F RNAi (#57868), EcR RNAi (#58286), vGAT RNAi (#41958), and tubP-GAL80ts (#7019). E93-RNAi, nSyb-GAL4, mCherry-RNAi lines were outcrossed for five generations to a Berlin background (originally from the Heisenberg lab). Flies were raised on standard cornmeal/molasses food and incubated at 25 °C under a 12 h light/12 h dark cycle.

Yip C., Wyler S.C., Liang K., Yamazaki S., Cobb T., Safdar M., Metai A., Merchant W., Wessells R., Rothenfluh A., Lee S., Elmquist J, & You Y.J. (2024). Neuronal E93 is required for adaptation to adult metabolism and behavior. Molecular Metabolism, 84, 101939.

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