Anti‐Pragmin polyclonal antibody A302‐675A (Bethyl Laboratories, Montgomery, TX, USA) was used as a primary antibody for immunoprecipitation, immunoblotting, and immunostaining. Anti‐Myc mAb 9E10 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti‐DDDDK polyclonal antibody PM020 (Medical & Biological Laboratories, Aichi, Japan), anti‐vinculin mAb hVIN‐1 (Sigma‐Aldrich, St. Louis, MO, USA) and anti‐Csk polyclonal antibody C‐20 (Santa Cruz Biotechnology) were used as primary antibodies for immunoblotting and immunostaining. Anti‐FLAG mAb M2 (Sigma‐Aldrich), anti‐phosphotyrosine mAb 4G10 (Millipore, Temecula, CA, USA), anti‐GST mAb B‐14 (Santa Cruz Biotechnology), anti‐His mAb 6C4 (Medical & Biological Laboratories), anti‐His mAb OGHis (Medical & Biological Laboratories), anti‐actin polyclonal antibody C‐11 (Santa Cruz Biotechnology), and anti‐p‐c‐Src (Y530) polyclonal antibody (Santa Cruz Biotechnology) were used as primary antibodies for immunoblotting.
Anti phosphotyrosine mab 4g10
Manufactured by Merck Group
Sourced in United States
Anti‐phosphotyrosine mAb 4G10 is a monoclonal antibody that binds to phosphorylated tyrosine residues. It is commonly used in Western blotting and immunoprecipitation experiments to detect and study proteins containing phosphorylated tyrosine moieties.
Automatically generated - may contain errors
Lab products found in correlation
Anti actin polyclonal antibody c 11, by Santa Cruz Biotechnology (1 mentions)
Anti flag m2 mab, by Merck Group (1 mentions)
Anti myc mab 9e10, by Santa Cruz Biotechnology (1 mentions)
Anti gst mab b 14, by Santa Cruz Biotechnology (1 mentions)
Bovine serum albumin (bsa), by Santa Cruz Biotechnology (1 mentions)
Halt protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti phosphotyrosine mab 4g10
1
Antibody Panel for Protein Detection
2
Preparation of anti-FcγRIIa monoclonal antibody
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The hybridoma producing the anti-FcγRIIa mAb, IV.3, was obtained from the American Type Culture Collection (Manassas, VA). Antibodies specific for Syk, Src and β-actin, and bovine serum albumin were purchased from Santa Cruz Biotechnology. Antibodies against focal adhesion kinase were from Thermo Scientific. Antibodies specific for Syk (phosphorylated tyrosine 525/526), Src (phosphorylated tyrosine 416) and Fak (phosphorylated tyrosine 397) were from Cell Signaling Technology. Anti-phosphotyrosine mAb 4G10 was purchased from Millipore. Fab fragments were prepared using a kit from Pierce Biotechnology. Phosphatase inhibitor cocktail was purchased from EMD Chemicals. Halt Protease inhibitor cocktail was purchased from Thermo Scientific. Human IgG was from Jackson ImmunoResearch Laboratories. Human fibrinogen was from Enzyme Research Laboratories Inc. Lyn and Fyn were obtained from Life Technologies. Src was purchased from Enzo Life Sciences.
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