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Hypoxic chamber

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The Hypoxic Chamber is a laboratory equipment designed to precisely control and maintain a low-oxygen environment. It is used to simulate conditions with reduced oxygen levels, enabling researchers to study the effects of hypoxia on various biological systems and cellular processes.

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Lab products found in correlation

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31 protocols using hypoxic chamber

1

Hypoxic and Normoxic Conditions in Mice

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Female C57BL6/J mice, 8–10 weeks (young) and 20 months (aged), were housed 4 to a cage, and placed into a hypoxic chamber (Biospherix) maintained at 8% O2 for periods up to 14 days. Littermate control mice were kept in the same room under similar conditions except that they were kept at ambient sea‐level oxygen levels (normoxia, approximately 21% O2 at sea‐level) for the duration of the experiment. Every few days, the chamber was briefly opened for cage cleaning and food and water replacement as needed.
Halder S.K, & Milner R. (2022). Exaggerated hypoxic vascular breakdown in aged brain due to reduced microglial vasculo‐protection. Aging Cell, 21(11), e13720.
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2

Hypoxia Exposure in C57BL/6J Mice

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C57BL/6J male mice, 8–10 weeks of age, were housed 4 to a cage, and placed into a hypoxic chamber (Biospherix, Redfield, NY) for periods up to 4 days maintained at 8% O2. Control mice were kept in the same room under similar conditions except that they were kept at ambient oxygen levels (normoxia) for the duration of the experiment. Every few days, the chamber was briefly opened for cage cleaning and food and water replacement as needed.
Burnier L., Boroujerdi A., Fernández J.A., Welser-Alves J.V., Griffin J.H, & Milner R. (2016). Physiological cerebrovascular remodeling in response to chronic mild hypoxia: a role for activated protein C. Experimental neurology, 283(Pt A), 396-403.
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3

Hypoxic Acclimation in Mice

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Wild-type C57Bl/6 littermate mice, 8-10 weeks of age, were housed 4 to a cage, and placed into a hypoxic chamber (Biospherix, Redfield, NY) for 7 days maintained at different oxygen levels of 8, 10, 12, 14, and 16% O2. Control mice were kept in the same room under similar conditions except that they were kept at ambient sea-level oxygen levels (normoxia, approximately 21% O2 at sea-level) for the duration of the experiment. In subsequent experiments, mice were maintained at 8% and 13% O2 for periods of 7 and 14 days. Every few days, the chamber was briefly opened for cage cleaning and food and water replacement as needed.
Boroujerdi A, & Milner R. (2014). Defining the critical hypoxic threshold that promotes vascular remodeling in the brain. Experimental neurology, 263, 132-140.
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4

Hypoxia Exposure in Aging Mice

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Female C57BL6/J mice, 8–10 weeks (young) and 20 months (aged), were housed 4 to a cage, and placed into a hypoxic chamber (Biospherix, Redfield, NY) maintained at 8% O2 for periods up to 14 days. Littermate control mice were kept in the same room under similar conditions except that they were kept at ambient sea-level oxygen levels (normoxia, approximately 21% O2 at sea-level) for the duration of the experiment. Every few days, the chamber was briefly opened for cage cleaning and food and water replacement as needed.
Halder S.K., Delorme-Walker V.D, & Milner R. (2023). β1 integrin is essential for blood–brain barrier integrity under stable and vascular remodelling conditions; effects differ with age. Fluids and Barriers of the CNS, 20, 52.
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5

Clonogenicity Assay under Hypoxia

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Cells were seeded (100 cells/well) into a 6 well plate and cultured for 10 days under normal culture conditions (21% O2) or hypoxia (1% O2, Biospherix hypoxic chamber). Clonogenicity was assessed by staining colonies with crystal violet and number of colonies counted using Image J and data expressed as cellular survival fraction.
Dier U., Shin D.H., Hemachandra L.P., Uusitalo L.M, & Hempel N. (2014). Bioenergetic Analysis of Ovarian Cancer Cell Lines: Profiling of Histological Subtypes and Identification of a Mitochondria-Defective Cell Line. PLoS ONE, 9(5), e98479.
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6

Chronic Hypoxia and Pharmacological Treatments

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Chronic hypoxic rearing was performed as previously described [35 (link), 48 (link), 53 (link), 54 (link)]. Briefly, litters of the C57Bl6/J strain were culled to a size of, at most, ten pups and co-fostered with CD1 or Swiss Webster strain dams then reared at 10% O2 in a hypoxic chamber (Biospherix, Inc., Laconia, NY) from postnatal day 3 (P3) to P11. Tissue from P4, P7, P11, P22, or P40 was then harvested acutely for analysis. On P3, pups received daily intraperitoneal injections of prednisolone (0.67 g/kg body weight, Sigma-Aldrich), SAG (20 g/kg body weight), prednisolone in combination with SAG, or vehicle (DMSO), for 8 days, or the duration of the hypoxic experiment. For acute treatment, a one-time dose of SAG was given at P11.
Nguyen V., Sabeur K., Maltepe E., Ameri K., Bayraktar O, & Rowitch D.H. (2017). Sonic Hedgehog Agonist Protects Against Complex Neonatal Cerebellar Injury. Cerebellum (London, England), 17(2), 213-227.
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7

Simulated Ischemia in C2C12, VSMC, and HUVEC

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C2C12 (ATCC, Manassas, VA, USA; cat# CRL-1772) and vascular smooth muscle cells (VSMC) were grown in Dulbecco’s Modified Eagle Medium (DMEM) + 10% fetal bovine serum (FBS) (Cell Applications, Inc., San Diego, CA, USA; cat# 250K-05n). Pooled human umbilical vein endothelial cells (HUVEC) were purchased from Cell Applications, Inc. (cat# 200K-05n) and grown in standard EC growth medium (Cell Applications, Inc.; cat# 211–500) with 10% FBS. Simulated ischemia was achieved, as previously described.15 (link),16 (link) Briefly, cells were grown under hypoxic conditions (2% oxygen; hypoxic chamber BioSpherix, Lacona, NY, USA) in EC serum starvation media (Cell Applications, Inc.; cat# 209–250) for 24 hours. Control cells (normoxia) were grown in 10% FBS EC media + 21% oxygen. To simulate ischemia in C2C12 and VSMC cells, they were grown in DMEM without serum + 2% oxygen while controls cells were grown in DMEM with 10% serum and 21% oxygen
Alleboina S., Ayalew D., Peravali R., Chen L., Wong T, & Dokun A.O. (2019). Dual specificity phosphatase 5 regulates perfusion recovery in experimental peripheral artery disease. Vascular medicine (London, England), 24(5), 395-404.
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8

Hypoxic Chamber for Mice Studies

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A hypoxic chamber (BioSpherix, Parish, NY, USA) was used to expose the mice to hypoxia (Figure 1A). In this chamber, the oxygen concentration was constantly maintained by an oxygen controller (BioSpherix), and the heater riser plate (BioSpherix) was set to warm up inside. The plate had two exhaust ducts and three fans, which circulated the air and rapidly homogenized the temperature inside. The plate contained a thermometer connected to a heat controller (BioSpherix), which was regulated to automatically adjust to the target temperature.
Zen R., Terashima T., Tsuji S., Katagi M., Ohashi N., Nobuta Y., Higuchi A., Kanai H., Murakami T, & Kojima H. (2022). Ambient Temperature Is Correlated With the Severity of Neonatal Hypoxic-Ischemic Brain Injury via Microglial Accumulation in Mice. Frontiers in Pediatrics, 10, 883556.
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9

Hypoxic Conditioning for Autoimmune Study

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Following immunization with PLP139–151 peptide, mice, housed 4 to a cage, were randomly divided into two groups: one was placed into a hypoxic chamber (Biospherix, Redfield, NY) maintained at 10% O2 for the duration of the experiment, while the control group was kept in the same room under similar conditions except that they were kept at ambient sea-level oxygen levels (normoxia, approximately 21% O2 at sea-level). Every day, the chamber was briefly opened to allow for clinical assessment of mice and cage cleaning and food and water replacement.
Halder S.K., Kant R, & Milner R. (2018). Hypoxic pre-conditioning suppresses experimental autoimmune encephalomyelitis by modifying multiple properties of blood vessels. Acta Neuropathologica Communications, 6, 86.
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10

Isolation and Culture of CD117+ Cells

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Samples were processed in accordance with the previous described method [18 (link)]. Briefly, cells from AFs were seeded on glass coverslips, and adherent cells were then positively selected for CD117 by using the MACS CD117 MicroBead Kit from Miltenyi Biotec (Bergisch Gladbach, Germany). Cells were cultured by using reconstituted Chang Medium C Lyophilized (Irvine Scientific, Santa Ana, CA, USA) before selection and then in expansion medium consisting of minimum essential medium alpha (Gibco, now part of Thermo Fisher Scientific, Waltham, MA, USA) containing 20 % Chang medium, 15 % fetal bovine serum (Gibco), antibiotics and L-Glutamine (2 mM final). Cells were maintained under standard 20 % O2, 5 % CO2 and 95 % relative humidity, referred to as “normoxia”. Cells were also cultured under “hypoxia” at 5 % O2 by using a CO2/O2 controller connected to a hypoxic chamber (BioSpherix, Lacona, NY, USA).
Schiavo A.A., Franzin C., Albiero M., Piccoli M., Spiro G., Bertin E., Urbani L., Visentin S., Cosmi E., Fadini G.P., De Coppi P, & Pozzobon M. (2015). Endothelial properties of third-trimester amniotic fluid stem cells cultured in hypoxia. Stem Cell Research & Therapy, 6, 209.
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