Bacto casamino acids

SOC medium containing 0.5% (w/v) yeast extract, 2% (w/v) tryptone, 10 mM NaCl, 2.5 mM KCl, 20 mM MgCl₂ and 20 mM D-glucose was used to recover E. coli and Salmonella cells following transformation. LB medium containing 0.5% (w/v) yeast extract, 1% (w/v) tryptone and 0.5% (w/v) NaCl supplemented with antibiotics at appropriate concentrations were used to grow E. coli and Salmonella strains. 2% (w/v) agarose was added to made the corresponding LB plates. SPI-2 inducing medium (pH 5.8) containing 170 mM 2-(N-morpholino)ethanesulfonic acid, 5 mM KCl, 7.5 mM (NH₄)₂SO₄, 0.5 mM K₂SO₄, 1 mM KH₂PO₄, 8 μM MgCl₂, 38 mM Glycerol and 0.1% (w/v) Bacto™ Casamino Acids (ThermoFisher Scientific, Cat# 223050) was used for Salmonella in vitro assay. DMEM (Gibco) containing 4 mM L-glutamine, 4,500 mg/L glucose, 1 mM sodium pyruvate, and 1,500 mg/L sodium bicarbonate, and additionally supplemented with 10% (w/v) fetal calf serum (FCS, Biochrom), 1% (w/v) sodium pyruvate, and antibiotics at appropriate concentrations was used to grow HeLa cells for Salmonella infection. Antibiotics were added at final concentrations of 100 μg/mL for Amp, 34 μg/mL for Cm, 50 μg/mL for Kan, 100 μg/mL for Hyg and 10 or 50 μg/mL gentamicin (Gm) for E. coli and S. Typhimurium. Final concentrations of 1 mM for IPTG and 20 mM for L-arabinose were used to induce the Sth1Cas9n base editor.