Nis elements advanced research

Manufactured by Nikon

Sourced in Japan, United States

NIS-Elements Advanced Research is a comprehensive software platform for imaging and analysis. It offers advanced tools for acquiring, processing, visualizing, and analyzing images from a wide range of microscopy techniques. The software supports multiple imaging modalities, including widefield, confocal, TIRF, and live-cell imaging.

Automatically generated - may contain errors

Lab products found in correlation

Eclipse ti, by Nikon (5 mentions) Imagej, by Nikon (2 mentions) Sylgard 184 silicone elastomer base, by Dow (1 mentions) Prism 7, by GraphPad (1 mentions) Bio plex manager software, by Bio-Rad (1 mentions) Eclipse te2000 e microscope, by Nikon (1 mentions) Ti e microscope, by Nikon (1 mentions) Vybrant cfda se cell tracer kit, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Avantor (1 mentions) Eclipse ti e microscope, by Nikon (1 mentions) Fluorescence microscope, by Nikon (1 mentions) Zen 2010, by Zeiss (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Originpro 8, by OriginLab (1 mentions) Chemotaxis and migration tool 2, by Ibidi (1 mentions) Coolsnap es2, by Nikon (1 mentions) A1rsi microscope, by Nikon (1 mentions) Orcaflash2, by Hamamatsu Photonics (1 mentions)

Spelling variants of this product

NIS-Elements (1183 citations) NIS Element Advanced Research software (15 citations)

23 protocols using nis elements advanced research

Quantifying Nuclear Localization of MPK-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

For all imaging, animals were mounted live in M9 buffer containing 2% tetramisole on slides with a 3% agar pad. DIC/Nomarski optics and fluorescence microscopy were captured using a Nikon A1si confocal microscope with 488, 561nm lasers (Figs. 2345; Figs. S1,2) or CSU-W1 spinningdisc confocal laser microscope with 488, 561nm lasers and Photometrics Prime BSI camera (Figs.
6, 7; Figs. S3,4). Slides prepared for the spinning-disc time-lapse captured were sealed with VALAP to prevent animals from drying out. Captured images were processed using NIS Elements Advanced research, version 4.40 (Nikon). Additional deconvolution processing was performed on all time-lapse images within the Nikon Elements software utilizing the 3-D Richardson-Lucy algorithm over 35 iterations.
To determine relative levels of nuclear localization of MPK-1, animals were imaged in 10minute intervals 24 hours post synchronization. Following deconvolution (above), fluorescent intensity measurements for both MPK-1::mKate2 and mNeonGreen::HIS-72 were recorded for each P4.p-P8.p nucleus using NIS Elements Advanced research, version 4.40 (Nikon) software. CTNF (corrected total nuclear fluorescence) was calculated by subtracting background fluorescence. To account for variations, we then divided the MPK-1::mKate2 CTNF intensity by their corresponding mNeonGreen::HIS-72 intensity. P-values were calculated using ANOVA.

Rasmussen N.R., & Reiner D.J. (2021). Nuclear translocation of tagged endogenous ERK/MPK-1 MAP Kinase denotes a subset of activation events inC. elegansdevelopment.

+ Open protocol

+ Expand

Single-Cell Analysis of Immune Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nanowells were fabricated using SYLGARD 184 silicone elastomer base (PDMS; Dow Corning, Midland, MI, USA) and a curing agent as described previously [17 (link)]. A suspension of 2 × 10⁵ cells in 100 μl of complete culture media was stained with CellTrace Calcein Violet, AM, for live cells and anti-CD19-Alexa Fluor (AF) 488 for 30 minutes on ice (Life Technologies, Carlsbad, CA, USA). Stained cells were washed in PBS and resuspended in 300 μl of culture media. The suspension of cells was loaded into an array of 50-μm nanowells. The cells were allowed to settle via gravity for 5 minutes. Excessive cells were rinsed off with media, and a LifterSlip coverslip (Fisher Scientific, Pittsburgh, PA, USA) was placed on top to prevent evaporation from the nanowells. The arrays were imaged using an automated epifluorescence microscope (Nikon Eclipse Ti; Nikon Instruments, Melville, NY, USA) equipped with a motorized stage, phase contrast, and 405-nm and 488-nm wavelength filter sets using Nikon NIS Elements Advanced Research image capture software.

Esfandiary L., Gupta N., Voigt A., Wanchoo A., Chan E.K., Sukumaran S, & Nguyen C.Q. (2016). Single-cell antibody nanowells: a novel technology in detecting anti-SSA/Ro60- and anti-SSB/La autoantibody-producing cells in peripheral blood of rheumatic disease patients. Arthritis Research & Therapy, 18, 107.

+ Open protocol

+ Expand

Confocal Microscopy Sample Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were pelleted and properly resuspended in 1X Phosphate buffer saline (PBS), placed on glass slide under coverslip, and scanned under microscope. A series of images were digitized with a confocal laser scanning microscope (A1r from Nikon) using different filters. The images were analyzed using Nikon NIS-Elements Advanced Research.

Abdelaal A.S, & Yazdani S.S. (2021). A genetic toolkit for co-expression of multiple proteins of diverse physiological implication. Biotechnology Reports, 32, e00692.

+ Open protocol

+ Expand

Quantitative Analysis of Cardiomyocyte Beating

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microscope images were processed and analyzed using the Nikon NIS-Elements Advanced Research (Nikon Europe B.V.) or ImageJ software. Beating patterns of CarMTs were analyzed using the Musclemotion macro (43 (link)) in ImageJ (National Institution of Health, Stapleton, NY, USA). We used the Bio-Plex Manager software (BioRad) and Microsoft Excel (Redmond, WA, USA) to analyze data obtained from the multiplex assay and the Troponin I ELISA. This data was statistically analyzed with one-way or two-way ANOVA depending on the data set and visualized using GraphPad Prism 7 software (GraphPad Software, San Diego, CA, USA). Data obtained from sMICA ELISA assay was processed and statistically analyzed with GraphPad Prism 7. All statistical results were represented as mean ± standard deviation (SD) with a significance of P < 0.05, unless indicated differently.

Nguyen O.T., Misun P.M., Lohasz C., Lee J., Wang W., Schroeder T, & Hierlemann A. (2021). An Immunocompetent Microphysiological System to Simultaneously Investigate Effects of Anti-Tumor Natural Killer Cells on Tumor and Cardiac Microtissues. Frontiers in Immunology, 12, 781337.

+ Open protocol

+ Expand

Live Cell Imaging of HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells expressing eGFP or SIRT4-eGFP (3 × 10⁵) were seeded on µ-Dish 35 mm plates (ibidi GmbH, Martinsried, Planegg). For live cell imaging, cells were cultured in CO₂-independent HEPES containing media (Life Technologies/Thermo Fisher Scientific, Schwerte, Germany) at 37 °C in an isolated incubation chamber essentially as described [44 (link)]. Cells were initially imaged at brightfield and 488 nm and thereafter only at brightfield every 12 min using a Nikon Eclipse TE2000-E microscope under control of the NIS Elements Advanced Research software (Nikon; version 4.20).

Bergmann L., Lang A., Bross C., Altinoluk-Hambüchen S., Fey I., Overbeck N., Stefanski A., Wiek C., Kefalas A., Verhülsdonk P., Mielke C., Sohn D., Stühler K., Hanenberg H., Jänicke R.U., Scheller J., Reichert A.S., Ahmadian M.R, & Piekorz R.P. (2020). Subcellular Localization and Mitotic Interactome Analyses Identify SIRT4 as a Centrosomally Localized and Microtubule Associated Protein. Cells, 9(9), 1950.

+ Open protocol

+ Expand

Retinal Vascular Imaging and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Retinal whole-mounts were imaged using the inverted fluorescent microscope Nikon Eclipse Ti (Nikon, Tokyo, Japan) with a digital camera CoolSNAP HQ2 (Photometrics, Tucson, AZ, U.S.A.) linked to a computer running the image analysis software ‘NIS-Elements Advanced Research’ (Nikon, Tokyo, Japan). Sequentially overlapping high-resolution images of the entire retinas were captured using the 4× objective. Images were merged to construct a montage of the retina. Montages were semi-quantified for vascular morphological abnormalities as previously published [4 (link)]. Grading of retinas were based on vascular changes such as regions of capillary dropout, vascular leakage, vessel tortuosity, monocytes/macrophages, microaneurysms, IRMA, exceptionally dense vascular budding and neovascular tufts. Representative areas of the retinal vascular lesions were captured in the z-plane in 2-μm steps. The z-stacks were compiled into a focused image representing all superficial, intermediate and deep capillary layers.

Matthews J., Herat L., Rooney J., Rakoczy E., Schlaich M, & Matthews V.B. (2022). Determining the role of SGLT2 inhibition with Empagliflozin in the development of diabetic retinopathy. Bioscience Reports, 42(3), BSR20212209.

+ Open protocol

+ Expand

Time-lapse Imaging of Cell Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phase contrast time-lapse imaging was conducted using a Ti-E Nikon microscope. The objective lens was 10x, NA = 0.3. Time-lapse imaging was performed using NIS-Elements Advanced Research (Nikon) software. A single frame was captured every 5 min during the first 10 h, followed by a frame rate of one frame per 15 min during the rest of the experiment.

Belansky J, & Yelin D. (2022). Optimization study of plasmonic cell fusion. Scientific Reports, 12, 7159.

+ Open protocol

+ Expand

Fluorescence Recovery After Photobleaching of Toxoplasma Parasites

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For FRAP experiments performed on tachyzoites, the pTub-GFP-HXGPRT56 (link), pTub-SOD2-Nterm-GFP-Myc (Nt-SOD2-GFP)40 (link) and GFP-HA-SET8 plasmids were transiently transfected into the parasites and the experiments were performed 24 h later for the RH strains or 48 h later for the ME49 strains. For the experiments performed on bradyzoites, ME49 strains stably expressing GFP were used and bradyzoite stage conversion was induced for 1–15 days. The experiments were performed on a Nikon A1r microscope (Ti Eclipse) controlled in temperature and CO₂. Acquisitions and processing were done with the softwares NIS-elements advanced research (Nikon) and ImageJ. The sequence used for the experiments was composed of an acquisition step of three images followed by two bleaches performed with 100% of laser (wavelength 488) and another acquisition step of 3–15 min with images collected every 5 or 10 s. All experiments were done at least in triplicates and 5–10 vacuoles were bleached each time.
All experiments were performed at the Bioimaging core facility of the Faculty of Medicine, University of Geneva.

Frénal K., Jacot D., Hammoudi P.M., Graindorge A., Maco B, & Soldati-Favre D. (2017). Myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of Toxoplasma gondii. Nature Communications, 8, 15710.

+ Open protocol

+ Expand

Murine Macrophage Isolation and Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine bone marrow-derived macrophages (BMDMs) were isolated from C57BL/6 mice femurs, flushed and cultured in DMEM with L929 fibroblast conditioned medium at 37°C and 5% CO₂ as described previously [24 (link)]. Macrophages used for experiments were 98% pure as assayed by flow cytometry using macrophage markers CD68APC and CD11bPE. Confluent macrophages were collected and stained with Vybrant® CFDA SE Cell Tracer Kit (Invitrogen, CA) as per manufacturer protocols and spiked onto BSO treated MAECs and VCECs. 15 minutes later, cells were triple washed with PBS (VWR, PA) and replaced with 10% FBS DMEM for imaging. Images were collected using a Nikon Eclipse Ti-E microscope (Nikon Instruments Inc., Melville, NY) and adherent macrophages were counted using the cell counting software feature on NIS Elements Advanced Research (Nikon Instruments Inc., Melville, NY).

Shrestha B., Prasai P.K., Kaskas A.M., Khanna A., Letchuman V., Letchuman S., Alexander J.S., Orr A.W., Woolard M.D, & Pattillo C.B. (2018). Differential Arterial and Venous Endothelial Redox Responses to Oxidative Stress. Microcirculation (New York, N.Y. : 1994), 25(7), e12486.

+ Open protocol

+ Expand

Confocal Microscopy Imaging and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Confocal image acquisition and analysis were obtained using Nikon Instruments Software (NIS)-Elements Advanced Research (Version 4.50; Nikon Instruments). Statistical analysis and data graphical representation was performed with GraphPad Prism (GraphPad Software Inc., Version 5.01 for Windows, San Diego, CA, USA, www.graphpad.com) and Microsoft Excel (Microsoft Corporation, 2010, https://office.microsoft.com/excel). Data were analyzed with an unpaired t-test or one-way ANOVA; a P-value ≤ 0.05 was considered as statistically significant. All experiments were independently repeated at least three times and data are presented as mean (SEM) or as median with range.

Sultan A.R., Lattwein K.R., Lemmens-den Toom N.A., Snijders S.V., Kooiman K., Verbon A, & van Wamel W.J. (2021). Paracetamol modulates biofilm formation in Staphylococcus aureus clonal complex 8 strains. Scientific Reports, 11, 5114.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!