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3 protocols using water soluble chitosan

1

Chitosan-Based Water-Soluble Polymer Synthesis

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Water-soluble chitosan was purchased from Biosynth Carbosynth (Compton, UK). All other chemicals were purchased from Merck KGaA (Darmstadt, Germany), unless differently stated, and used as received without further purifications. Deionized water (conductivity less than 0.1 S) was prepared with an inverse osmosis system (Culligan, Milan, Italy). PolyCAL TM Pullulan std-57k was used (Malvern Instruments Ltd., Malvern, UK). The reagent grades were 98%.
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2

Triamcinolone-Chitosan Nanoparticles for Delivery

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Triamcinolone acetonide (TA) (MW: 434.50 g/mol with purity >99%) and water-soluble chitosan (MW: 10–100 KDa with a deacetylation degree >90%) were procured from Carbosynth Ltd., Berkshire, UK. PLGA (DL-lactide/Glycolide copolymer, ratio M/M%: 50/50, MW: 38,000–54,000), Pluronic® F-127 (MW: ~12,600 g/mol), poly (vinyl alcohol) (MW: 89,000–98,000), dichloromethane (purity ≥99.8%), tween® 80, sodium azide (purity >99.5%) and phosphate buffer saline tablets (0.01 M phosphate buffer), were purchased from Sigma Aldrich, Arklow, Ireland.
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3

Glioblastoma Cell Viability Assay

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Gelatin (type A), 5-methylfurfural, 4arm-PEG10K-Maleimide (Star-PEG-MA), Phosphate-Buffered Saline, U87 glioblastoma cell line, Eagle's minimal essential medium, L-glutamine, Sodium Pyruvate, Fetal Bovine Serum, penicillin, and streptomycin were purchased from Sigma-Aldrich, Italy. Water-soluble chitosan was purchased from Carbosynth Ltd, UK. A LIVE/DEAD Cell Viability Assay was purchased from ThermoFisher.
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