Anti-p-TAK1 (9339), anti-TAK1 (5206), anti-p-P38 (9211), anti-P38 (9212), anti-p-JNK (9251), anti-JNK (9252), anti-p65 (8242) anti-p-p65 (3033), anti-IκBα, anti-p-IKKα/β (14938), anti-IRAK1 (4504), and anti-MyD88 (4283) were from Cell Signaling Technology (Danvers, MA, USA); Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and 0.25% trypsin were purchased from Gibco BRL (Grand Island, NY, USA). 3-[4,5-Dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), lipopolysaccharide (LPS), tumor necrosis factor α (TNFα), and IRAK1/4 inhibitor (C20H21N5O4) were purchased from Sigma Chemical Co. (St. Louis, MO, USA); IL-1β and TNFα enzyme-linked immune sorbent assay (ELISA) kit were from R&D systems (Minneapolis, MN, USA).
Anti myd88 4283
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-MyD88 (4283) is a primary antibody that recognizes the human MyD88 protein. MyD88 is an adaptor protein involved in the signaling pathways of various Toll-like receptors and interleukin-1 receptor family members.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (1 mentions)
Anti irak1 4504, by Cell Signaling Technology (1 mentions)
Myc agarose beads, by Merck Group (1 mentions)
Mouse il 12p40 elisa kit, by R&D Systems (1 mentions)
Anti nfatc3 a303 572a, by Fortis Life Sciences (1 mentions)
Mouse ifn α elisa kit, by Thermo Fisher Scientific (1 mentions)
Anti hdac1 5356, by Cell Signaling Technology (1 mentions)
Cpg dna, by Merck Group (1 mentions)
On targetplus sirna, by GE Healthcare (1 mentions)
Ha agarose beads, by Merck Group (1 mentions)
Glutathione sepharose 4b beads, by GE Healthcare (1 mentions)
Anti ha antibody, by Merck Group (1 mentions)
Anti β actin, by Abcam (1 mentions)
2 protocols using anti myd88 4283
1
Molecular Signaling Pathway Analysis
2
Immunoblot and Immunoprecipitation Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CpG DNA were purchased from Sigma-Aldrich. The ON-TARGETplus siRNA were from GE Healthcare. The following antibodies were used for immunoblot analysis and/or immunoprecipitation: anti-IRF7 (sc-74472), anti-IRF7 (sc-9083), anti-NFATC1 (sc-13033), anti-NFATC2 (sc-7296), anti-NFATC3 (sc-8321), anti-NFATC4 (sc-13036), and anti-NFAT5 (sc-13035) were from Santa Cruz Biotechnology, Inc.; anti-IRF7 (51–3300) was from Thermo Fisher Scientific; anti-MyD88 (4283), anti–NF-κB1 p105/p50, and anti-HDAC1 (5356) were from Cell Signaling Technology; and anti-NFATC3 (A303-572A) was from Bethyl Laboratories, Inc. HRP-conjugated anti-HA (ab1265), anti-Myc (ab62928), and anti-transferrin receptor antibody (ab84036) were from Abcam; anti–β-actin, anti-HA antibodies, anti-GST HRP conjugate antibody, HA–agarose beads, and Myc–agarose beads were purchased from Sigma-Aldrich. Glutathione Sepharose 4B beads were from GE Healthcare. Human IFN-α, TNF-α, and IL-6 ELISA kits were from Mabtech. The mouse IFN-α ELISA kit was from eBioscience. The mouse IL-12p40 ELISA kit was from R&D Systems.
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