Tim3 bv510 clone f38 2e2

Manufactured by Thermo Fisher Scientific

TIM3-BV510 (clone F38–2E2) is a flow cytometry reagent that detects the expression of the TIM3 (T-cell immunoglobulin and mucin-domain containing-3) protein on the surface of cells. TIM3 is an inhibitory receptor that plays a role in the regulation of immune responses. The BV510 fluorochrome is used to label the antibody, enabling its detection and analysis by flow cytometry.

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Lab products found in correlation

Cd45ra fitc or bv711 clone hi100, by BD (3 mentions) Ccr7 bv421 clone 150503, by BD (3 mentions) Cd122 bv510 clone mik β3, by BD (3 mentions) Cd45ro pe cy7 clone uchl1, by BD (2 mentions) Cd4 buv395 clone sk3, by BD (2 mentions) Cd8 buv805 clone sk1, by BD (2 mentions) Cd22 fc and her2 fc recombinant proteins, by R&D Systems (2 mentions) Dylight488 and or 650 antibody labeling kits, by Thermo Fisher Scientific (2 mentions) Cd3 pacblue clone hit3a, by Thermo Fisher Scientific (2 mentions) Cd45 percp cy5.5 clone hi30, by BD (2 mentions) Cd62l bv605 clone dreg 56, by BD (2 mentions) Cd8 percp cy5, by Thermo Fisher Scientific (1 mentions) Pd 1 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd45ro pe cy7, by BD (1 mentions) Cd4 buv395, by BD (1 mentions) Cd3 pe, by Thermo Fisher Scientific (1 mentions) Cd8 buv805, by BD (1 mentions) Cd62l bv605, by BD (1 mentions) Cd4 apc cy7, by BioLegend (1 mentions) Cd45 percp cy5, by BD (1 mentions)

3 protocols using tim3 bv510 clone f38 2e2

Comprehensive Immunophenotyping of T Cell Subsets

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The anti-CD19 CAR idiotype antibody was kindly provided by Bipulendu Jena and Laurence Cooper⁴⁸. The 1A7 anti-14G2a idiotype antibody was obtained from NCI-Frederick. CD22 and Her2 CARs were detected using human CD22-Fc and Her2-Fc recombinant proteins (R&D). The idiotype antibodies and Fc-fusion proteins were conjugated in house with Dylight488 and/or 650 antibody labeling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
From BioLegend: CD4-APC-Cy7 (clone OKT4), CD8-PerCp-Cy5.5 (clone SK1), TIM3-BV510 (clone F38–2E2), CD39-FITC or APC-Cy7 (clone A1), CD95-PE (clone DX2), CD3-PacBlue (clone HIT3a),
From eBioscience: PD1-PE-Cy7 (clone eBio J105), LAG3-PE (clone 3DS223H), CD45RO-PE-Cy7 (clone UCHL1), CD45-PerCp-Cy5.5 (clone HI30),
From BD: CD45RA-FITC or BV711 (clone HI100), CCR7-BV421 (clone 150503), CD122-BV510 (clone Mik-β3), CD62L-BV605 (clone DREG-56), CD4-BUV395 (clone SK3), CD8-BUV805 (clone SK1).

Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.

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Comprehensive Immunophenotyping of T Cell Subsets

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Multiparameter Flow Cytometry Analysis

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The following antibodies were used (from BioLegend): CD4-APC-Cy7 (clone OKT4), CD8-PerCp-Cy5.5 (clone SK1), TIM-3-BV510 (clone F38-2E2), CD39-FITC or APC-Cy7 (clone A1), CD95-PE (clone DX2), CD3-PacBlue (clone HIT3a), PD-1-BV421 (clone EH12.2H7), and CD3-PE (clone HIT3a); (from eBioscience): PD-1-PE-Cy7 (clone eBio J105), LAG-3-PE (clone 3DS223H), CD45RO-PE-Cy7 (clone UCHL1), and CD45-PerCp-Cy5.5 (clone HI30); (from BD): CD45RA-FITC or BV711 (clone HI100), CCR7-BV421 (clone 150503), CD122-BV510 (clone Mik-β3), CD62L-BV605 (clone DREG-56), CD4-BUV395 (clone SK3), and CD8-BUV805 (clone SK1).
The anti–CD19-28z CAR antibody was provided by B. Jena and L. Cooper, Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX. The anti–HA-28z CAR antibody was provided by the National Cancer Institute, Frederick, MD. Each was conjugated with Dylight288 and/or 650 antibody labeling kits (Thermo Fisher).

Gennert D.G., Lynn R.C., Granja J.M., Weber E.W., Mumbach M.R., Zhao Y., Duren Z., Sotillo E., Greenleaf W.J., Wong W.H., Satpathy A.T., Mackall C.L, & Chang H.Y. (2021). Dynamic chromatin regulatory landscape of human CAR T cell exhaustion. Proceedings of the National Academy of Sciences of the United States of America, 118(30), e2104758118.

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