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Surveyor ic human total survivin immunoassay

Manufactured by R&D Systems
Sourced in United States

The Surveyor™ IC Human Total Survivin Immunoassay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of total survivin levels in human cell culture supernates, serum, and plasma samples.

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2 protocols using surveyor ic human total survivin immunoassay

1

Quantification of Survivin in SINE-Treated Cells

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Survivin protein was quantified in whole or nuclear/cytosolic cell lysate obtained from cells exposed to SINE in the presence or absence of subtoxic concentrations of Bortezomib (1 nmol/L; Selleck Chemicals; #S1013) using Surveyor™ IC Human Total Survivin Immunoassay (R&D Systems; #SUV647) according to the manufacturer's protocol.
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2

Quantification of Survivin and PARP-1 Cleavage

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Survivin protein was quantified in nuclear/cytosolic cell lysate obtained from cells exposed to selinexor using Surveyor IC Human Total Survivin Immunoassay (#SUV647; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. Nuclear ubiquitinated survivin, obtained by immunoprecipitation with an anti-human survivin antibody (#ab469; Abcam) was quantified in the nuclear fraction, using CycLex Poly-Ubiquitinated Protein ELISA Kit (MBL International), according to manufacturer’s protocol. Cleaved PARP-1 were quantified using Pierce Cleaved PARP-1 Colorimetric In-Cell ELISA Kit (#62219; Thermo Fisher Scientific Inc., Waltham, MA, USA), according to the manufacturer’s protocol.
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