For immunoprecipitation experiments, 500 μg of nuclear extract was incubated for 4 h at 4 °C with 4 μg of anti-NME2 antibody (Kamiya, KM1121 MC412). Immunoprecipitation was performed using a Catch and Release co-immunoprecipitation kit (Millipore) according to the manufacturer's protocol.
Catch and release co immunoprecipitation kit
Manufactured by Merck Group
The Catch and Release co-immunoprecipitation kit is a laboratory tool designed to facilitate the isolation and purification of protein complexes from cell lysates or tissue samples. The kit includes all the necessary components to perform co-immunoprecipitation experiments, allowing researchers to study protein-protein interactions in their native cellular environment.
Automatically generated - may contain errors
2 protocols using catch and release co immunoprecipitation kit
1
Immunoprecipitation of NME2 Protein
2
TRF2, LSD1, and RAP1 Interactions
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HT-1080 cells were collected and washed in cold 1X PBS and nuclear extract was isolated using nuclear extract kit (Cell Extract from Sigma) as per manufacturer protocol. For immunoprecipitation experiments 500 µg of nuclear extract was incubated for 4 hours at 4 °C with 5 µg of anti-TRF2 antibody (Novus Biological) immunoprecipitation was performed using Catch and Release co-immunoprecipitation kit (Millipore) as per manufacturer’s protocol using anti-LSD1 antibody (Cell Signalling Technology) and anti-RAP1 antibody (Santacruz biotechnology). For reverse CoIP, 5 µg of LSD1 was used for immunoprecipitation.
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