Log In Sign up
The largest database of trusted experimental protocols

Mtbstfa 1 tbdmcs

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

MTBSTFA + 1% TBDMCS is a silylation reagent used for the derivatization of compounds containing active hydrogen atoms, such as alcohols, phenols, and carboxylic acids, prior to gas chromatographic (GC) analysis. It is designed to improve the volatility and thermal stability of these compounds, making them more suitable for GC analysis.

Automatically generated - may contain errors

Lab products found in correlation

Propionic anhydride, by Merck Group (2 mentions) Lc ms grade water, by Avantor (2 mentions) Lc ms grade acetonitrile, by Avantor (2 mentions) Ethanol absolute uv ir hplc pai, by Thermo Fisher Scientific (2 mentions) D glucose, by Merck Group (2 mentions) Pyridine, by Merck Group (2 mentions) Glutamine, by Merck Group (1 mentions) U 13c6 glucose, by Merck Group (1 mentions) Ponceau s, by Merck Group (1 mentions) Trypsin, by Merck Group (1 mentions) Glucose, by Merck Group (1 mentions)

3 protocols using mtbstfa 1 tbdmcs

1

Metabolic Flux Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
[U-13C6]-glucose, and unlabeled sucrose, glucose, glutamine, asparagine plant protease inhibitor cocktail, Ponceau-S, trypsin and all common buffer reagents were purchased from Sigma (Milwaukee, WI). SDS-Page reagents were purchased from Invitrogen, (Carlsbad, CA). MTBSTFA +1% TBDMCS were purchased from Thermo Scientific (Waltham, MA). Uniformly labeled 7% glucose was obtained as a custom order from Cambridge Isotope Laboratories.
Allen D.K., Evans B.S, & Libourel I.G. (2014). Analysis of Isotopic Labeling in Peptide Fragments by Tandem Mass Spectrometry. PLoS ONE, 9(3), e91537.
+ Open protocol
+ Expand
2

Isotopically Labeled Glucose Derivatives

Check if the same lab product or an alternative is used in the 5 most similar protocols
D-glucose was obtained from Sigma-Aldrich (St. Louis, MO, USA). [2-18O]glucose (> 90% enrichment), [5-18O]glucose (> 90% enrichment), and [6-18O]glucose (> 90% enrichment) were obtained from Omicron Biochemicals, Inc. (South Bend, IN, USA). [U-13C6, U-2H7]glucose (99% and 97%–98% enriched, respectively) was obtained from Cambridge Isotope Laboratories Inc. (Andover, MA, USA) and purchased through Tracertec (Madrid, Spain). 18O-water at 97% enrichment was obtained from Sercon Ltd (Crewe, UK). Acetonitrile (LC–MS grade) and water (LC–MS grade) were acquired from VWR (Radnor, PA, USA), while ethanol (absolute [UV-IR-HPLC PAI]) was acquired from Fisher Chemical (Waltham, MA, USA). Hydroxylamine hydrochloride was obtained from Acros Organics from Thermo Fisher Scientific (NJ, USA). Propionic anhydride and pyridine were procured from Sigma Aldrich and Thermo Fisher Scientific, respectively. Silylation reagent (MTBSTFA + 1% TBDMCS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Coelho M., Mahar R., Belew G.D., Torres A., Barosa C., Cabral F., Viegas I., Gastaldelli A., Mendes V.M., Manadas B., Jones J.G, & Merritt M.E. (2022). Enrichment of hepatic glycogen and plasma glucose from H₂18O informs gluconeogenic and indirect pathway fluxes in naturally feeding mice. Nmr in Biomedicine, 36(2), e4837.
+ Open protocol
+ Expand
3

Isotopically Labeled Glucose Acquisition

Check if the same lab product or an alternative is used in the 5 most similar protocols
D‐glucose was obtained from Sigma‐Aldrich (St. Louis, MO, USA). [2‐18O]glucose (> 90% enrichment), [5‐18O]glucose (> 90% enrichment), and [6‐18O]glucose (> 90% enrichment) were obtained from Omicron Biochemicals, Inc. (South Bend, IN, USA). [U‐13C6, U‐2H7]glucose (99% and 97%–98% enriched, respectively) was obtained from Cambridge Isotope Laboratories Inc. (Andover, MA, USA) and purchased through Tracertec (Madrid, Spain). 18O‐water at 97% enrichment was obtained from Sercon Ltd (Crewe, UK). Acetonitrile (LC–MS grade) and water (LC–MS grade) were acquired from VWR (Radnor, PA, USA), while ethanol (absolute [UV‐IR‐HPLC PAI]) was acquired from Fisher Chemical (Waltham, MA, USA). Hydroxylamine hydrochloride was obtained from Acros Organics from Thermo Fisher Scientific (NJ, USA). Propionic anhydride and pyridine were procured from Sigma Aldrich and Thermo Fisher Scientific, respectively. Silylation reagent (MTBSTFA + 1% TBDMCS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Coelho M., Mahar R., Belew G.D., Torres A., Barosa C., Cabral F., Viegas I., Gastaldelli A., Mendes V.M., Manadas B., Jones J.G, & Merritt M.E. (2022). Enrichment of hepatic glycogen and plasma glucose from H₂18O informs gluconeogenic and indirect pathway fluxes in naturally feeding mice. Nmr in Biomedicine, 36(2), e4837.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!