Stimulated PBMC were washed and incubated for 30 minutes on ice with directly conjugated antibodies (CD3: SK7, R&D Systems; CD45: T29/33, BD Biosciences; HLA-DR: TU36, Life technologies) in combination with a dead cell marker (Live/Dead Fixable near IR; Molecular Probes). Analyses were done using a Beckton Dickinson LSRII SORP flow cytometer and FlowJo 9.5.3.
Lsrii sorp flow cytometer
Manufactured by FlowJo
Sourced in United States
The LSRII SORP flow cytometer is a high-performance instrument designed for multiparameter flow cytometry analysis. It is equipped with multiple lasers and detectors, enabling the simultaneous measurement of various cellular parameters, such as size, granularity, and the expression of multiple fluorescently-labeled markers. The LSRII SORP flow cytometer is a versatile and powerful tool for advanced cell analysis in research and clinical applications.
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Lab products found in correlation
2 protocols using lsrii sorp flow cytometer
1
Flow Cytometric Immunophenotyping of PBMCs
2
Multiparametric Flow Cytometry Analysis
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Stimulated cells were washed and incubated for 30 min on ice with conjugated antibodies: anti-CD3-FITC (clone SK7, R&D Systems), anti-CD45-Pacific Blue (clone T29/33, BD Biosciences) and anti-HLA-DR-Qdot 605 (clone TU36; Life Technologies) in combination with the dead cell marker Live/Dead Fixable near IR (Molecular Probes). Analyses were performed using a Beckton Dickinson LSRII SORP flow cytometer and FlowJo 9.5.3.
All cell types were stained with conjugated anti-ADAM10-PE (BioLegend, San Diego, CA, USA) and compared with their isotype control. Fluorescence was detected by flow cytometry analysis using same laser settings for all different cell types.
All cell types were stained with conjugated anti-ADAM10-PE (BioLegend, San Diego, CA, USA) and compared with their isotype control. Fluorescence was detected by flow cytometry analysis using same laser settings for all different cell types.
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