Mcherry rab5ca q79l
MCherry-Rab5CA(Q79L) is a fluorescent protein construct that consists of the constitutively active mutant form of the Rab5 GTPase, Rab5CA(Q79L), fused to the mCherry red fluorescent protein. Rab5 is a small GTPase that regulates early endosome formation and trafficking. The Q79L mutation renders Rab5 constitutively active, leading to the formation of enlarged endosomes.
Lab products found in correlation
4 protocols using mcherry rab5ca q79l
Fluorescent Rab Protein Constructs
Fluorescent Protein-Tagged Rab GTPases
Nsg1-Emerald:Addgene cat#54202, Michael Davidson lab; the mutation at residue 114 from D to G was corrected.
Nsg1-mCherry:mouse full length Nsg1 was cloned at the N-terminal of mCherry in pcDNA3 vector.
Nsg2-GFP:mouse full length Nsg2 was cloned into pcDNA-GFP by Genscript
mRFP-Rab5:Addgene cat#14437, Ari Helenius lab.
mCherry Rab5CA(Q79L) cat#35138, mCherry Rab5DN(S34N) cat#35139:Addgene, Sergio Grinstein lab.
EGFP-Rab7 cat#12605, EGFP-Rab7DN(T22N) cat#12660:Addgene, Richard Pagano labmTagBFP2-Rab5, cat#56417, Addgene, Michael Davidson lab;
mTagBFP2-Rab7:Michael Davidson lab.
GFP:Clontech
mCherry:Clontech.
Cloning and Tagging of Golgi Proteins
Rab5-Mediated Vesicle Dynamics Protocol
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