All reagents were obtained from LabBox (Vilassar de Dalt, Spain). All phytohormones, unlabeled (GA1, GA3, GA4, GA7, GA8, GA12, GA15, GA19, GA20, GA29, GA44, GA51, GA53, ABA, JA, IAA) and deuterium-labeled (d2-GA1, d2-GA3, d2-GA4, d2-GA7, d2-GA8, d2-GA12, d2-GA15, d2-GA19, d2-GA20, d2-GA29, d2-GA44, d2-GA51, d2-GA53, d6-ABA, d6-JA, d5-IAA) standards, were purchased from OlChemIm (Olomouc, Czechia). SPE columns and OASIS® HLB 1cc and OASIS® PRIME HLB 1cc were purchased from Waters (Milford, MA, United States). Fixed insert vials and pre-slit PTFE screw cap were purchased from Teknokroma (Sant Cugat del Vallès, Spain). The HPLC column Kinetex® 2.6 μm XB-C18 100 Å (30∗2.1 mm) was purchased from Phenomenex (Torrance, CA, United States), and the HPLC column Mediterranea Sea 18 column (10∗0.2 cm, 2.2 μm) was purchased from Teknokroma (Sant Cugat del Vallès, Spain).
D2 ga29
Manufactured by OlChemIm
Sourced in Czechia
D2-GA29 is a laboratory instrument designed for the measurement and analysis of chemical compounds. It is a high-performance liquid chromatography (HPLC) system specifically engineered for the separation, identification, and quantification of various substances. The core function of the D2-GA29 is to provide accurate and reliable data for researchers and scientists working in the field of analytical chemistry.
Automatically generated - may contain errors
Lab products found in correlation
D2 ga15, by OlChemIm (2 mentions)
D2 ga19, by OlChemIm (2 mentions)
D2 ga4, by OlChemIm (2 mentions)
D2 ga8, by OlChemIm (2 mentions)
D2 ga20, by OlChemIm (2 mentions)
Oasis hlb 1cc, by Waters Corporation (1 mentions)
Oasis prime hlb 1cc, by Waters Corporation (1 mentions)
Kinetex 2.6 μm xb c18 100, by Phenomenex (1 mentions)
Mediterranea sea18 column, by Teknokroma (1 mentions)
D6 aba, by OlChemIm (1 mentions)
D5 iaa, by OlChemIm (1 mentions)
Acquity beh c18 column, by Waters Corporation (1 mentions)
Acquity tqd, by Waters Corporation (1 mentions)
D2 ga1, by OlChemIm (1 mentions)
D2 ga9, by OlChemIm (1 mentions)
Thermomixer, by Eppendorf (1 mentions)
2 protocols using d2 ga29
1
Comprehensive Phytohormone Profiling Protocol
2
Quantifying Wheat Grain Gibberellins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Wheat samples
(100 mg taken from 10 flash-frozen, pooled, and magnalised grains)
were extracted in triplicate overnight in 1.0 mL of acidified methanol
pH 4.0 [80/20, methanol/5.0 mM formic acid-containing butylated hydroxytoluene
(3–5 crystals)]. As internal tracers, d2-GA1, d2-GA4, d2-GA8,
d2-GA9, d2-GA15, d2-GA19, d2-GA20, and d2-GA29 (20 pmol each, Olchemim)
were added. After purification on an RP-C18 cartridge (500 mg), samples
were derivatized with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (1.0 mg/sample, pH 4.0, 60 min,
37 °C under continuous shaking, Eppendorf thermomixer). Next,
these derivatized samples were analyzed using a UPLC-MS/MS equipped
with an electrospray interface in positive mode (ACQUITY, TQD, Waters).
Samples (6.0 μL) were injected on an ACQUITY BEH C18 column
(2.1 × 50 mm; 1.7 mm, Waters) using a column temperature of 30
°C and eluted at 450 μL/min with the following gradient
of 0.1% formic acid in water (solvent A) and 0.1% formic acid in acetonitrile
(solvent B): 0–0.8 min isocratic 92% A, 8% B; 0.8–5
min linear gradient to 60%A, 40%B; 5–5.5 min linear gradient
to 10% A, 90% B.
(100 mg taken from 10 flash-frozen, pooled, and magnalised grains)
were extracted in triplicate overnight in 1.0 mL of acidified methanol
pH 4.0 [80/20, methanol/5.0 mM formic acid-containing butylated hydroxytoluene
(3–5 crystals)]. As internal tracers, d2-GA1, d2-GA4, d2-GA8,
d2-GA9, d2-GA15, d2-GA19, d2-GA20, and d2-GA29 (20 pmol each, Olchemim)
were added. After purification on an RP-C18 cartridge (500 mg), samples
were derivatized with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (1.0 mg/sample, pH 4.0, 60 min,
37 °C under continuous shaking, Eppendorf thermomixer). Next,
these derivatized samples were analyzed using a UPLC-MS/MS equipped
with an electrospray interface in positive mode (ACQUITY, TQD, Waters).
Samples (6.0 μL) were injected on an ACQUITY BEH C18 column
(2.1 × 50 mm; 1.7 mm, Waters) using a column temperature of 30
°C and eluted at 450 μL/min with the following gradient
of 0.1% formic acid in water (solvent A) and 0.1% formic acid in acetonitrile
(solvent B): 0–0.8 min isocratic 92% A, 8% B; 0.8–5
min linear gradient to 60%A, 40%B; 5–5.5 min linear gradient
to 10% A, 90% B.
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