Perfluorobutanesulfonic acid

Manufactured by Merck Group

Sourced in United States

Perfluorobutanesulfonic acid is a fluorinated organic compound. It is a white crystalline solid that is soluble in water and organic solvents. This substance can be used as a chemical intermediate or as a component in various industrial and laboratory applications.

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2 protocols using perfluorobutanesulfonic acid

PFOS and PFBS Quantification in C. elegans

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Perfluorooctanesulfonic acid (PFOS, ~40% in H₂O, catalog no. 77283, lot 101406188), and perfluorobutanesulfonic acid (PFBS, 97%, catalog no. 562629, lot MKCB6204), Analytical standard of PFOS (98%) and PFBS (97%), ampicillin and carbenicillin were purchased from Sigma-Aldrich Co. (St. Louis, MO). The internal standard (13C8 PFOS, 50 μg/mL in methanol) was purchased from Cambridge Isotope Laboratories (Andover, MA). Luria-Bertani medium, biological agar and peptone were purchased from Fisher Scientific Inc. (Pittsburgh, PA). Commercial kits (Triglyceride, Infinity™ Triglyceride Reagent) used for triglyceride measurement and Hoechst 33258 (>98%) used for DNA quantification were purchased from Thermo Fisher Scientific Inc. (Middletown, VA). For protein quantification, Bio-Rad DC protein assay kit was purchased from Bio-Rad Co. (Hercules, CA). Escherichia coli OP50 and C. elegans strains including N2, Bristol (wild type) and DH1033 (sqt-1(sc103) II; bIs1 X), were obtained from Caenorhabditis Genetics Center (CGC).

Yue Y., Li S., Qian Z., Pereira R.F., Lee J., Doherty J.J., Zhang Z., Peng Y., Clark J.M., Timme-Laragy A.R, & Park Y. (2020). Perfluorooctanesulfonic acid (PFOS) and perfluorobutanesulfonic acid (PFBS) impaired reproduction and altered offspring physiological functions in Caenorhabditis elegans. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 145, 111695.

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Plasma Protein Binding of PFAS Compounds

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The molecular structures of the 14 PFAS evaluated are in Figure S1 and physiochemical properties, such as molecular weight (MW) and logD shown in Table S1. The following fourteen PFAS were either obtained from Accustandard, Inc. (New Haven, CT, USA) or Sigma-Aldrich (St. Louis, MO, USA): Perfluorobutanoic acid (PFBA), perfluoropentanoic acid (PFPA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUDA), perfluorododecanoic acid (PFDoDA), perfluorohexanesulfonic acid (PFHxS), perfluorooctanesulfonamide (PFOSA), 6:2 fluorotelomer sulfonate (6:2 FtS), perfluorobutanesulfonic acid (PFBS), and perfluorooctanesulfonic acid (PFOS). Human, rat (Wistar-Han), and mouse (CD-1; C57BL/6) equal sex pooled (minimum of 3 male and 3 female) plasma was obtained from BioIVT (Hicksville, NY, USA). The equilibrium dialysis device (HTD96) and cellulose membranes with a molecular weight cutoff of 12–14 kDa were obtained from HTDialysis, LLC (Gales Ferry, CT, USA). Human, rat, and mouse albumin, along with all other reagents were obtained from Sigma-Aldrich unless specified otherwise.

Ryu S., Burchett W., Zhang S., Modaresi S.M., Agudelo Areiza J., Kaye E., Fischer F.C, & Slitt A.L. (2024). Species-Specific Unbound Fraction Differences in Highly Bound PFAS: A Comparative Study across Human, Rat, and Mouse Plasma and Albumin. Toxics, 12(4), 253.

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