Dihydro β erythroidine dhβe

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Dihydro‐β‐erythroidine (DHβE) is a compound that acts as a selective antagonist of nicotinic acetylcholine receptors. It is commonly used as a research tool in the study of nicotinic receptor function and signaling.

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Dihydro-β-erythroidine hydrobromide (DHβE) (27 citations) Dihydro-β-erythroidine (2 citations)

4 protocols using dihydro β erythroidine dhβe

Pharmacological Modulation of Nicotinic Receptors

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d‐glucose, calcium d‐gluconate, DMSO, ACh, ATP, mecamylamine, NaHCO₃, papaine, l‐cysteine, leupeptine, pyruvic acid, NaOH and tyrphostin AG490 (AG 490) were acquired from Sigma‐Aldrich (Taufkirchen, Germany). A‐85380, α‐bungarotoxin, α‐conotoxin PnIA, α‐conotoxin MII, chelerythrine chloride, dihydro‐β‐erythroidine (DHβE), epibatidine, T16Ainh‐A01, Ani 9, CaCCinh‐A01, Chromanol293B, ACV 1 (conotoxin Vc1.1), dantrolene, JTV519 and H‐89 were obtained from Tocris Bioscience (Abingdon, UK). The α‐conotoxin ImI was ordered from Alomone Labs (Jerusalem, Israel). KH₂PO₄, KCl and MgCl₂ were purchased from MERCK (Darmstadt, Germany). NaCl was ordered from Grüssing GmbH (Westoverledingen, Germany). HEPES and BSA were from Carl Roth (Karlsruhe, Germany). Thapsigargin and WP1066 were acquired from Cayman Chemicals (Ann Arbor, MI, USA) and nicotine from Glentham Life Sciences (Corsham, UK). EDTA and Tween 20 were obtained from VWR (Darmstadt, Germany). Sodium pyruvate was purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA) and DNase1 from Invitrogen (Thermo Fisher Scientific).

Kumar P., Scholze P., Fronius M., Krasteva‐Christ G, & Hollenhorst M.I. (2020). Nicotine stimulates ion transport via metabotropic β4 subunit containing nicotinic ACh receptors. British Journal of Pharmacology, 177(24), 5595-5608.

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Neurotransmitter Modulation Protocol

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Trihexyphenidyl, atropine, nicotine, neostigmine, ʟ-DOPA, and dopamine were obtained from Sigma-Aldrich (St. Louis, MO). Dihydro-β-erythroidine (DHβE), ambenonium, and CNQX were obtained from Tocris (Minneapolis, MN).

Downs A.M., Fan X., Donsante C., Jinnah H.A, & Hess E.J. (2019). Trihexyphenidyl rescues the deficit in dopamine neurotransmission in a mouse model of DYT1 dystonia. Neurobiology of disease, 125, 115-122.

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Pharmacological Evaluation of Nicotinic Receptor Modulators

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Doses are expressed as the weight, in mg/kg, of the forms listed below, except in the case of nicotine, which is expressed as the weight of the free base. Nicotine hydrogen tartrate salt (Sigma-Aldrich, St. Louis, MO), mecamylamine (Waterstone Technology, Carmel, IN), dihydro-β-erythroidine (DHβE; Tocris Bioscience, Bristol, UK), methyllycaconitine citrate (MLA; Research Technology Branch of the National Institute on Drug Abuse, Rockville, MD), and desformylflustrabromine hydrochloride (dFBr; Tocris) were dissolved in physiological saline. LY 2087101 (Tocris) and PNU-120596 (Research Technology Branch of the National Institute on Drug Abuse) were dissolved in 1 part propylene glycol (Fisher Scientific, Hampton, NH): 1 part Tween 80 (Sigma-Aldrich): 18 parts saline. Drugs were administered s.c. in a volume of 10 ml/kg except for mecamylamine, LY 2087101, and PNU-120596, which were administered i.p. Due to limitations of solubility, doses of 100 mg/kg LY 2087101 and 100 mg/kg PNU-120596 were administered in a volume of 20 ml/kg and their effects were compared to an equivalent volume of vehicle. Doses for each compound were selected based on the literature, when available. Drugs were studied from ineffective doses up to a dose producing discriminative stimulus, rate-decreasing, or hypothermic effects or up to the limit of drug solubility.

Moerke M.J., de Moura F.B., Koek W, & McMahon L.R. (2016). Effects of nicotine in combination with drugs described as positive allosteric nicotinic acetylcholine receptor modulators in vitro: discriminative stimulus and hypothermic effects in mice. European journal of pharmacology, 786, 169-178.

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Pharmacological Agents for Neurophysiology

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ω-conotoxin GVIA (CTX), ω-agatoxin IVA (ATX), nifedipine, quinpirole and sulpiride, were obtained from Sigma-Aldrich. (1S, 2S)-2-[2-[[3-(1H-Benzimidazol-2-yl) propyl] methylamino]ethyl]-6-fluoro- 1, 2, 3, 4-tetrahydro-1-(1-methylethyl)-2-naphthalenyl cyclopropanecarboxylate dihydrochloride (NNC 50-0396), Cyclopiazonic acid (CPA) and dihydro-β-erythroidine (DHβE) were obtained from Tocris Cookson. Drugs were dissolved as stock solutions in water or DMSO and aliquoted and frozen at −20 °C before use. Each of the drugs was diluted in aCSF immediately before each experiment. When used, the final concentration of DMSO external solution was always <0.05%.

Sgobio C., Sun L., Ding J., Herms J., Lovinger D.M, & Cai H. (2019). Unbalanced calcium channel activity underlies selective vulnerability of nigrostriatal dopaminergic terminals in Parkinsonian mice. Scientific Reports, 9, 4857.

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