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Nanodrop 1000 8000 spectrophotometers

Manufactured by Thermo Fisher Scientific
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The NanoDrop™ 1000/8000 spectrophotometers are compact instruments designed for the measurement of absorbance of small-volume samples. The NanoDrop™ 1000 measures sample volumes as low as 1 μL, while the NanoDrop™ 8000 can measure up to 8 samples simultaneously.

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Lab products found in correlation

2100 bioanalyzer, by Agilent Technologies (4 mentions) Rna 6000 pico kit, by Agilent Technologies (3 mentions) Ht rna assay reagent kit, by PerkinElmer (3 mentions) Labchip gx bioanalyser, by PerkinElmer (3 mentions) Tri reagent, by Merck Group (1 mentions) Tempus spin rna isolation kit, by Thermo Fisher Scientific (1 mentions) Ribopure kit, by Thermo Fisher Scientific (1 mentions) Tempus reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

NanoDrop (8392 citations) NanoDrop spectrophotometer (6460 citations) NanoDrop 1000 spectrophotometer (3243 citations) NanoDrop 1000 (2999 citations) NanoDrop 8000 (633 citations) NanoDrop 8000 spectrophotometer (573 citations)

4 protocols using nanodrop 1000 8000 spectrophotometers

1

Microarray and RNA-seq Sample Preparation

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Quantity was verified using NanoDrop™ 1000/8000 spectrophotometers (Thermo Fisher Scientific). Quality and integrity of the total and the globin-reduced RNA were assessed with the HT RNA Assay Reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS) or RNA 6000 Pico kit (Agilent) using a BioAnalyzer 2100 (Agilent) and assigned an RNA Integrity (RIN) score. RNA with an RQS/RIN >6 was used to prepare samples for microarray or RNA-seq.
Supplementary Data 20 provides details of each sample provided including QC data such as the RIN, RNA conc, 260/280 ratio, # reads sequenced, # reads aligned to genome, # reads aligned to genes by HTSeq, etc.
Singhania A., Graham C.M., Gabryšová L., Moreira-Teixeira L., Stavropoulos E., Pitt J.M., Chakravarty P., Warnatsch A., Branchett W.J., Conejero L., Lin J.W., Davidson S., Wilson M.S., Bancroft G., Langhorne J., Frickel E., Sesay A.K., Priestnall S.L., Herbert E., Ioannou M., Wang Q., Humphreys I.R., Dodd J., Openshaw P.J., Mayer-Barber K.D., Jankovic D., Sher A., Lloyd C.M., Baldwin N., Chaussabel D., Papayannopoulos V., Wack A., Banchereau J.F., Pascual V.M, & O’Garra A. (2019). Transcriptional profiling unveils type I and II interferon networks in blood and tissues across diseases. Nature Communications, 10, 2887.
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2

RNA Quality Assessment for Microarray and RNA-seq

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantity was verified using NanoDrop™ 1000/8000
spectrophotometers (Thermo Fisher Scientific). Quality and integrity of the
total and the globin-reduced RNA were assessed with the HT RNA Assay Reagent kit
(Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin
Elmer) and assigned an RNA Quality Score (RQS) or RNA 6000 Pico kit (Agilent)
using a BioAnalyzer 2100 (Agilent) and assigned an RNA Integrity (RIN) score.
RNA with an RQS/RIN >6 was used to prepare samples for microarray or
RNA-seq.
Moreira-Teixeira L., Tabone O., Graham C.M., Singhania A., Stavropoulos E., Redford P.S., Chakravarty P., Priestnall S.L., Suarez-Bonnet A., Herbert E., Mayer-Barber K.D., Sher A., Fonseca K.L., Sousa J., Cá B., Verma R., Haldar P., Saraiva M, & O’Garra A. (2020). Mouse transcriptome reveals potential signatures of protection and pathogenesis in human tuberculosis. Nature immunology, 21(4), 464-476.
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3

Transcriptome Analysis from Biological Samples

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Blood was collected in Tempus reagent (Life Technologies) at 1:2 ratio. Total RNA was extracted using the Tempus™ Spin RNA isolation kit (Thermo Fisher Scientific). Globin RNA was depleted from total RNA (1.5–2 µg) using the GLOBINclear™-Mouse/Rat Globin mRNA removal kit (Thermo Fisher Scientific). Lungs were collected in TRI-Reagent (Sigma-Aldrich). Total RNA was extracted using the RiboPure™ Kit (Ambion). RNA quantity was verified using NanoDrop™ 1000/8000 spectrophotometers (Thermo Fisher Scientific). Quality and integrity of the total and the globin-reduced RNA were assessed using a BioAnalyzer 2100 (Agilent).
Moreira-Teixeira L., Stimpson P.J., Stavropoulos E., Hadebe S., Chakravarty P., Ioannou M., Aramburu I.V., Herbert E., Priestnall S.L., Suarez-Bonnet A., Sousa J., Fonseca K.L., Wang Q., Vashakidze S., Rodríguez-Martínez P., Vilaplana C., Saraiva M., Papayannopoulos V, & O’Garra A. (2020). Type I IFN exacerbates disease in tuberculosis-susceptible mice by inducing neutrophil-mediated lung inflammation and NETosis. Nature Communications, 11, 5566.
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4

RNA Quality Assessment for Microarray and RNA-seq

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantity was verified using NanoDrop™ 1000/8000
spectrophotometers (Thermo Fisher Scientific). Quality and integrity of the
total and the globin-reduced RNA were assessed with the HT RNA Assay Reagent kit
(Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin
Elmer) and assigned an RNA Quality Score (RQS) or RNA 6000 Pico kit (Agilent)
using a BioAnalyzer 2100 (Agilent) and assigned an RNA Integrity (RIN) score.
RNA with an RQS/RIN >6 was used to prepare samples for microarray or
RNA-seq.
Moreira-Teixeira L., Tabone O., Graham C.M., Singhania A., Stavropoulos E., Redford P.S., Chakravarty P., Priestnall S.L., Suarez-Bonnet A., Herbert E., Mayer-Barber K.D., Sher A., Fonseca K.L., Sousa J., Cá B., Verma R., Haldar P., Saraiva M, & O’Garra A. (2020). Mouse transcriptome reveals potential signatures of protection and pathogenesis in human tuberculosis. Nature immunology, 21(4), 464-476.
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