Fluorescein isothiocyanate labeled insulin fitc insulin

Recombinant human insulin, fluorescein isothiocyanate-labeled insulin (FITC-insulin), and SGDC were purchased from Sigma-Aldrich (St Louis, MO, USA). The TR146 cell line was obtained from the European Collection of Authenticated Cell Cultures (ECACC 10032305; Public Health England, Salisbury, UK). Ham’s F-12 nutrient, fetal bovine serum (FBS), trypsin-ethylenediaminetetraacetic acid (EDTA), Dulbecco’s phosphate-buffered saline (DPBS), Hanks’ balanced salt solution (HBSS), and 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES) were obtained from WELGENE Inc. (Gyeongsan, Republic of Korea). All other chemicals were of reagent grade and were used without further purification.

Human insulin was purchased from ProSpec-Tany Techno-Gene Ltd. (Ness-Ziona, Israel). Soy lecithin (Solec 2F-UB) was purchased from Bunge North America, Inc. (Chesterfield, MO, USA). Sodium cholate (SC) and STC were purchased from Alfa Aesar (Heysham, UK). SGC and sodium deoxytaurocholate (SDTC) were purchased from Acros Organics (Morris Plains, NJ, USA). Fluorescein isothiocyanate-labeled insulin (FITC-insulin) and sodium deoxyglycocholate (SDGC) were purchased from Sigma-Aldrich (St Louis, MO, USA). TR146 cell line was purchased from Public Health England (London, UK). Hank’s balanced salt solution (HBSS), fetal bovine serum (FBS), Ham’s F-12 nutrient, and trypsin–ethylenediaminetetraacetic acid (EDTA; 0.25%) were purchased from WELGENE Inc. (Gyeongsan, Republic of Korea). All other chemicals were of reagent-grade and used without further purification.