Chicken egg white albumin

Manufactured by Merck Group

Sourced in United States

Chicken egg white albumin is a laboratory reagent derived from the egg white of chickens. It is a natural protein that serves as a standard for various analytical and research applications.

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Lab products found in correlation

Horseradish peroxidase hrp conjugated goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Donkey anti goat igg, by Jackson ImmunoResearch (2 mentions) Essential fatty acid free bovine serum albumin, by BD (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Penicillin streptomycin, by Lonza (2 mentions) Goat anti mouse igg, by Jackson ImmunoResearch (2 mentions) Trypsin edta, by Lonza (2 mentions) Fetal bovine serum (fbs), by Lonza (2 mentions) Mmp 12, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal β actin antibody, by Merck Group (1 mentions) Roswell park memorial institute (rpmi), by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Avantor (1 mentions) Rpmi 1640 media, by Corning (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Il 13, by Thermo Fisher Scientific (1 mentions) Il 10, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Corning (1 mentions)

5 protocols using chicken egg white albumin

Investigating Inflammatory Mediators in Cell Lines

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RPMI, chicken egg white albumin, and LPS were obtained from the Sigma-Aldrich Chemical (St. Louis, MO, USA), as were all other reagents, unless specifically stated elsewhere. Fetal bovine serum (FBS), penicillin-streptomycin, and trypsin-EDTA were purchased from the Lonza (Walkersville, MD, USA). Rabbit polyclonal antibodies of matrix metalloproteinase (MMP)-12 and intracellular adhesion molecule (ICAM)-1, goat polyclonal cyclooxygenase (COX)-2 antibody, and mouse monoclonal inducible NOS (iNOS) antibody were purchased from the Santa Cruz Biotechnology (Dallas, TX, USA). Mouse monoclonal antibodies of bcl-2 and bax were provided by BD Transduction Laboratories (Franklin Lakes, NJ, USA). Rabbit polyclonal antibodies of cleaved caspase-3, cleaved caspase-9, phospho-p53, inhibitory κB (IκB), and phospho-IκB were obtained from Cell Signaling Technology (Beverly, MA, USA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG, donkey anti-goat IgG, and goat anti-mouse IgG were purchased from Jackson Immuno-Research Laboratories (West Grove, PA, USA). Mouse monoclonal β-actin antibody was obtained from Sigma-Aldrich Chemicals. Essential fatty acid free bovine serum albumin (BSA) and skim milk were supplied by Becton Dickinson Company (Sparks, MD, USA). 4’,6-Diamidino-2-phenylindole (DAPI) was obtained from Santa Cruz Biotechnology.

Kim Y.H., Kang M.K., Lee E.J., Kim D.Y., Oh H., Kim S.I., Oh S.Y., Kim K.H., Park S.J., Choi Y.J, & Kang Y.H. (2019). Dried Yeast Extracts Curtails Pulmonary Oxidative Stress, Inflammation and Tissue Destruction in a Model of Experimental Emphysema. Antioxidants, 8(9), 349.

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Alveolar Macrophage Polarization Protocol

Cited 3 times

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Isolated alveolar macrophages were suspended in RPMI-1640 media (Corning; Corning, NY, USA) supplemented with 10% fetal bovine serum (FBS) (VWR; Radnor, PA, USA), 1% penicillin-streptomycin (Corning) and 1% sodium pyruvate (Corning). Cells were suspended at 1×10⁵ cells/well with 100 μl/well for 96-well plate formats, 2×10⁵ cells/well with 400 μl/well for 24-well plate formats, and 5×10⁵ cells/well with 3 ml/well for 6-well plate formats. For macrophage phenotype polarization, lavaged cells were polarized into the different phenotypes and incubated at 37°C in a 5% CO₂ incubator for 24-hr as follows: M1 [20 ng/ml IFNγ (PeproTech; Rocky Hill, NJ, USA) + 10 pg/ml LPS (Genin et al., 2015 (link))], M2a [20 ng/ml IL-4 (PeproTech) + 20 ng/mL IL-13 (PeproTech) (Genin et al., 2015 (link))], M2b [immune complex; IC, consisting of 150 μg/ml anti-chicken egg albumin antibody (Sigma-Aldrich) + 15 μg/ml chicken egg white albumin (Sigma-Aldrich), incubated at 37°C for 30 min to form IC, and then 50 ng/ml LPS was added (Yue et al., 2017 (link))], or M2c [20 ng/ml IL-10 (eBioscience; San Diego, CA, USA) + 20 ng/mL TGF- β (R&D Systems; Minneapolis, MN, USA) (Kim et al., 2019 (link); Zizzo et al., 2012 (link))]. Treatments for each experiment are described below.

Fletcher P., Hamilton RF J.r., Rhoderick J.F., Pestka J.J, & Holian A. (2020). Docosahexaenoic acid impacts macrophage phenotype subsets and phagolysosomal membrane permeability with particle exposure. Journal of toxicology and environmental health. Part A, 84(4), 152-172.

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Optimized Cell Culture Conditions

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M199, human epidermal growth factor (EGF), hydrocortisone, gelatin, human insulin, apotransferrin, chicken egg white albumin and LPS were obtained from Sigma-Aldrich Chemical (St. Louis, MO, USA), as were all other reagents, unless specifically stated otherwise. Fetal bovine serum (FBS), penicillin-streptomycin and trypsin-EDTA were purchased from Lonza (Walkersville, MD, USA). Eotaxin-1 (CCL11) antibody was purchased from R&D systems (Minneapolis, MN, USA) and β-actin antibody was obtained from Sigma-Aldrich Chemicals. Additionally, MUC5AC antibody was provided by Abcam (Cambridge, UK). Antibodies of toll-like receptor 4 (TLR4), intracellular adhesion molecule (ICAM)-1 and eosinophil major basic protein (EMBP) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG, donkey anti-goat IgG and goat anti-mouse IgG were purchased from Jackson Immuno-Research Laboratories (West Grove, PA, USA). Essential fatty acid free bovine serum albumin (BSA) and skim milk were supplied by Becton Dickinson Company (Sparks, MD, USA).

Kim Y.H., Choi Y.J., Lee E.J., Kang M.K., Park S.H., Kim D.Y., Oh H., Park S.J, & Kang Y.H. (2017). Novel glutathione-containing dry-yeast extracts inhibit eosinophilia and mucus overproduction in a murine model of asthma. Nutrition Research and Practice, 11(6), 461-469.

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Visualizing Platelet Actin Cytoskeleton

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Platelets were diluted to a final concentration of 2 × 10⁴ cells/mL in cell culture medium (DMEM, Sigma-Aldrich, Vienna, Austria) and were allowed to settle on a glass slide for 15 min. Non-adherent cells were washed away with Phosphate-Buffered Saline (PBS). Actin cytoskeleton was visualized using Alexa Fluor 647 phalloidin (Cell Signaling Technology, Leiden, The Netherlands) in a Cytoskeleton Buffer with Sucrose (CBS) containing 10 mM MES pH 6.1, 138 mM KCl, 3 mM MgCl₂, 2 mM EGTA, and 0.32 M sucrose according to a protocol from Louise Cramer [23 (link)] (MRC Laboratory for Molecular Cell Biology, UCL, London, UK). Briefly, platelets were fixed using 4% paraformaldehyde in CBS for 20 min at room temperature, then permeabilized using 0.5% Triton X-100 with CBS, blocked in 10% chicken-egg-white-albumin (Sigma-Aldrich, Vienna, Austria) and stained for 20 min with 66 nM Alexa Fluor 647 conjugated to phalloidin.

Hauser F, & Jacak J. (2021). Real-time 3D single-molecule localization microscopy analysis using lookup tables. Biomedical Optics Express, 12(8), 4955-4968.

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Rapeseed Press Cake Characterization

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Cold-pressed rapeseed press cake (RPC) (Brassica napus L.) was a kind gift from Gunnarshögs Jordbruk AB (Hammenhög, Sweden). The rapeseeds were cold-pressed at Gunnarshögs Jordbruk AB’s production site and the oil temperature during pressing did not exceed 35 °C and no solvents were used. The hot-pressed rapeseed meal (RM) and the solvent-extracted RM was a kind gift from AAK AB (Karlshamn, Sweden). Hot pressing was typically performed at 80 °C to 100 °C. For the solvent-extracted meal, the oil residues were recovered by hexane post hot pressing. Citric acid (C₆H₈O₇, CAS 77-92-9), sodium chloride (NaCl, CAS 7647-14-5), sodium dihydrogen phosphate monohydrate (H₂Na PO₄·H₂O, CAS 7558-80-7), disodium hydrogen phosphate dodecahydrate (Na₂HPO₄·12H₂O, CAS 10039-32-4), and sodium hydroxide (NaOH, CAS 1310-73-2) were purchased from Merck (Darmstadt, Germany). Miglyol 812 was purchased from Sasol AG (Witten, Germany). Bovine serum albumin (BSA) and chicken egg white albumin were purchased from Sigma-Aldrich (St. Louis, MA, USA) and deoiled lecithin from soy was purchased from Cargill (Minneapolis, MN, USA). All other chemicals were of analytical grade.

Östbring K., Malmqvist E., Nilsson K., Rosenlind I, & Rayner M. (2019). The Effects of Oil Extraction Methods on Recovery Yield and Emulsifying Properties of Proteins from Rapeseed Meal and Press Cake. Foods, 9(1), 19.

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