Quemesa

Samples were prepared as previously described^{74 (link)}. In brief, PAO1 was incubated in CA-MHB overnight at 37 °C. After centrifugation, bacteria were resuspended in fresh CA-MHB containing NV716 at different concentrations during 1 h. Bacteria were fixed in 1% glutaraldehyde in 0.1 M phosphate buffer for 4 h. After post-fixation in 1% osmium tetroxide for 1 h and dehydration in ethanol, samples were embedded in resin. 70 nm sections were cut with a Leica ultracut UCT ultramicrotome and examined (unstained) in a JEOL 1400 transmission electron microscope equipped with an 11 Mpxl EMSIS Quemesa camera.

2x PEG precipitated virion samples normalized to 1 × 10¹⁰ virions/ml were prepared for microscopy as described18 (link). Images were recorded in a Philips CM100 transmission electron microscope at 80 kV using a Olympus Quemesa camera or in a JEOL1400Plus at 80 kV using a Ruby camera. A measuring grid was overlayed and intersections with the grid were counted. The length of each particle was calculated using π/4 * I * d (I being the number of intersections and d being the distance between the grid lines)61 .