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Peg300

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PEG300 is a polyethylene glycol with an average molecular weight of 300 g/mol. It is a clear, colorless, viscous liquid that is commonly used as a solvent, emulsifier, and lubricant in various applications.

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Lab products found in correlation

Tween 80, by MedChemExpress (6 mentions) Dimethyl sulfoxide (dmso), by MedChemExpress (3 mentions) Co2 incubator, by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Quizartinib, by Targetmol (1 mentions) Gilteritinib, by Targetmol (1 mentions) Fibroblast growth factor 2 (fgf2), by Thermo Fisher Scientific (1 mentions) Lps l2880, by Merck Group (1 mentions) Gw4064, by MedChemExpress (1 mentions) Peg300, by Maclin Biochemical Technology (1 mentions) F 12 dmem, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Tween 80, by Merck Group (1 mentions) Microinjection pump, by RWD Life Science (1 mentions) Dimethylsulfoxide dmso, by MedChemExpress (1 mentions) Drp1 antibody, by Abcam (1 mentions)

16 protocols using peg300

1

Preparation and Dosing of Tyrosine Kinase Inhibitors

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Sitravatinib, gilteritinib and quizartinib were purchased from TargetMol (Boston, USA). For in vitro experiments, all the drugs were reconstituted in DMSO to 10 mM for storage and further diluted in the culture medium with the final concentration of DMSO below 0.1%. For in vivo animal experiments, gilteritinib and quizartinib were suspended in a 0.5% methylcellulose solution and sitravatinib was dissolved in a formula containing 5% DMSO, 30% PEG300, 10% Tween 80 and 55% sterile water. DMSO, methylcellulose, PEG300, Tween 80 and busulfan were provided by MedChemExpress (Monmouth Junction, USA). FL and FGF2 were provided by PeproTech (Cranbury, USA).
Zhang Y., Wang P., Wang Y, & Shen Y. (2023). Sitravatinib as a potent FLT3 inhibitor can overcome gilteritinib resistance in acute myeloid leukemia. Biomarker Research, 11, 8.
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2

Investigating Liver Injury Mechanisms

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GW4064, PEG300, Tween 80 and GCDCA were purchased from MedChemExpress. LPS (L2880) was purchased from Sigma. CCl4 was purchased from Lianlong Bohua (Tianjin) Pharmaceutical Chemistry Co., Ltd. (Tianjin, China).
Feng S., Xie X., Li J., Xu X., Chen C., Zou G., Lin G., Huang T., Hu R., Ran T., Han L., Zhang Q., Li Y, & Zhao X. (2024). Bile acids induce liver fibrosis through the NLRP3 inflammasome pathway and the mechanism of FXR inhibition of NLRP3 activation. Hepatology International, 18(3), 1040-1052.
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3

Efficacy of Combination Therapy in PDX Models

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Once the PDX tumors grew to a volume of 100−300 mm3, mice were divided into groups randomly and treated with different drugs. Based on the mice's weight, TG003 (30 mg/kg) and Olaparib (50 mg/kg) were administered via intraperitoneal (i.p.) injection every 2 days. Olaparib was administered 30 min prior to TG003. Cisplatin (5 mg/kg) was administered via i.p. injection twice a week. The endpoint for tumor transplantation was determined when a tumor reached 1.5 cm in any dimension. To determine the therapeutic efficacy of the drugs in the PDX models, tumor volumes were monitored using calipers every 2−3 days and calculated using the equation (L × W2)/2, where L stands for length and W for width. Four weeks after treatment, mice were euthanized, and the tumors and some organs were harvested for future analysis.
For in vivo treatment, Olaparib was suspended in 4% dimethyl sulfoxide (DMSO; #D806648; Macklin) + 30% PEG300 (#HY‐Y0873; MedChemExpress) in H2O. TG003 was suspended in 5% DMSO + 40% PEG300 + 5% Tween80 (#T6336; Macklin) in H2O. Cisplatin was dissolved directly in H2O. Olaparib and TG003 were stored with parafilm at −20°C and cisplatin was stored at 4°C in dark storage.
Jiang Y., Huang S., Zhang L., Zhou Y., Zhang W., Wan T., Gu H., Ouyang Y., Zheng X., Liu P., Pan B., Xiang H., Ju M., Luo R., Jia W., Huang S., Li J, & Zheng M. (2024). Targeting the Cdc2‐like kinase 2 for overcoming platinum resistance in ovarian cancer. MedComm, 5(4), e537.
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4

Tissue Explant Culture for IL-6 and Corylifol A Study

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The tissue culture samples were collected and prepared as described in a previous study [11 (link)]; the tissues were cut into small blocks, washed twice with F-12/DMEM (Invitrogen, USA), centrifuged (1500 rpm, 5 min), and then cultured in F-12/DMED (Invitrogen) supplemented with 10% FBS (Gibco; Australia) and 1% penicillin–streptomycin (Invitrogen). Tissue explants were then placed into a CO2 incubator (37 °C, 5% CO2, approximately 0.1 g explants) (Thermo Fisher Scientific, USA) and cultured with 2 mL culture medium. IL-6 (PEPROTECH, USA) was diluted in 0.1% BSA (Sigma–Aldrich, USA) and PBS (Sigma–Aldrich); Corylifol A (MedChemExpress, USA) was diluted with 10% DMSO (Sigma–Aldrich), 40% PEG300 (MedChemExpress), 5% Tween-80 (Sigma–Aldrich) and 45% saline (Sigma–Aldrich).
Wang R., Zhao S., Chen X., Xiao Z., Wen X., Zhong X., Li S., Cheng H, & Huang G. (2022). Molecular mechanisms involved in the IL-6-mediated upregulation of indoleamine 2,3-dioxygenase 1 (IDO1) expression in the chorionic villi and decidua of women in early pregnancy. BMC Pregnancy and Childbirth, 22, 983.
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5

Hypertonic Dedifferentiation of Adipocytes

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To induce hypertonic shock, adipocytes were treated with a medium containing 2% polyethylene glycol 300 (PEG 300) diluted in culture medium. Specifically, adipocytes were exposed to the hypertonic dedifferentiation medium, composed of DMEM supplemented with 10% FBS and 1% penicillin–streptomycin (all obtained from Thermo Fisher) and 2% PEG-300 (MedChemExpress, product no. HY-Y0873), and maintained at 37 °C and 5% CO2. The medium was changed every 3 days, being careful to avoid shaking the dish to allow the hypertonic medium to diffuse slowly in the culture dish.
Liu G., Wang Y., Pan Y., Tian L., Choi M.H., Wang L., Kim J.Y., Zhang J., Cheng S.H, & Zhang L. (2023). Hypertonicity induces mitochondrial extracellular vesicles (MEVs) that activate TNF-α and β-catenin signaling to promote adipocyte dedifferentiation. Stem Cell Research & Therapy, 14, 333.
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6

Cannabinoid Receptor Modulation in ACC

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Mice anaesthetized with 1.25% avertin (20 ml/kg) were fixed on a stereotaxic tube, the cannula (62,204, RWD, China) was embedded 1 mm deep in the right ACC brain region, and then the mice were placed in cages alone for 2 weeks to recover. Ten minutes before EA, 300 nl of CB1R antagonist AM251 was diluted to a concentration of 2.5 mg/mL (4.50 mM) using 100 μl Dimethyl sulfoxide (DMSO) + 400 μl PEG300 + 50 μl Tween-80 (MedChemExpress, United States) injected into the ACC region at a rate of 30 nl/min through a microinjection pump (RWD, China) connecting the cannula. The control group was injected with the same amount of artificial cerebrospinal fluid by the same method. The behavioural test was performed 30 min after EA.
Wu J., Hua L., Liu W., Yang X., Tang X., Yuan S., Zhou S., Ye Q., Cui S., Wu Z., Lai L., Tang C., Wang L., Yi W., Yao L, & Xu N. (2023). Electroacupuncture Exerts Analgesic Effects by Restoring Hyperactivity via Cannabinoid Type 1 Receptors in the Anterior Cingulate Cortex in Chronic Inflammatory Pain. Molecular Neurobiology, 61(5), 2949-2963.
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7

Topical LY3023414 Dose Evaluation

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To determine the topical dose of LY3023414 (Cat. No.: HY-12513, MedChem Express, Monmouth Junction, NJ, USA), four different concentrations, 0.5, 1.0, 2.5, and 5.0%, of LY3023414, dissolved in polyethylene glycol (PEG-300, Cat. No. 202371, Sigma Aldrich, Saint Louis, MO, USA), were applied topically to the anus of mice (N=3 per group) for two consecutive weeks, five days a week. 1% LY3023414 was the lowest concentration that resulted in a decrease in pAKT and pS6 expression, markers of the PI3K and mTOR activity respectively, as assessed by immunohistochemical staining (data not shown). Subsequently, all mice receiving topical LY3023414 were treated five days a week, Monday-Friday, topically at the anus with 20 μL of 1% LY3023414 in PEG-300.
Gunder L.C., Moyer T.H., Ziolkowski M.R., Keating M.K., Leverson G.E., Zhang W, & Carchman E.H. (2022). Systemic Delivery of a Dual PI3K/mTOR Inhibitor More Effective than Topical Delivery in Preventing Anal Carcinogenesis in an HPV Transgenic Mouse Model. Journal of cancer science and clinical therapeutics, 6(2), 157-173.
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8

Nrf2-Mediated Mitochondrial Dynamics Regulation

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The reagents and apparatus were purchased from the following companies: the NBP (Catalog No. HY-B0647, 200 mg/tube), Tert-butylhydroquinone (TBHQ) (Catalog No. HY-100489, 500 mg/tube), Brusatol (Bru) (Catalog No. HY 19543, 10 mg/tube), PEG 300 (Catalog No. HY-Y0873, 100 ml/vial) and HO-1 antibody (HY-P70276) were purchased from MedChemExpress Co. Ltd.; caspase-3 antibody (4.1.18) (Catalog No. SC-65497), caspase-9 antibody (96.1.23) (Catalog No. SC-56076), Mfn1 antibody (D-10) (Catalog No. Sc-166644) and Mfn2 antibody (XX-1) (Catalog No. sc-100560) from Santa Co. Ltd.; Nrf2 polyclonal antibody (Catalog No. 16396-1-AP) from Proteintech Co. Ltd.; Drp1 antibody (recombinant Anti-Drp1 antibody) (Catalog No. Ab184248) from Abcam Co. Ltd.,; beta actin Polyclonal Antibody (Catalog No. E-AB--20058), Goat Anti-Rabbit IgG (H+L) (Catalog No. E-AB-1003), Goat Anti-Mouse IgG (H+L) (Catalog No. E-AB-1001) from Elabscience Co. Ltd. The Tecan Safire2 full-wavelength multifunctional microplate reader was purchased from Tecan Group Co. Ltd., Switzerland, and the FUSION FX7 multifunctional imaging system was purchased from Vilber Co. Ltd., France.
He J., Gao J., Zhu H., Zhao Y., Zhang X., Wang X., Wan S., Cao H., Zhai L., Wang Y, & Wang S. (2023). Effects of NBP on postoperative cognitive dysfunction in rats via Nrf 2/ARE pathway. Aging (Albany NY), 15(1), 276-286.
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9

Investigating Estrogen Receptor Regulation in POA

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Four weeks after treatment, 60 rats (n = 20 per group) were randomly selected and equally divided into ERα group and ERβ group. Five rats were randomly selected from the SHAM group and OVX + E group of ERα group, the antagonists of ERα (AZD9496, HY-12870, MedChemExpress) were injected into POA, and the agonists of ERα (Propyl pyrazole triol, HY-100689, MedChemExpress) were injected into POA of the five rats randomly selected from the OVX group. Five rats were randomly selected from SHAM group and OVX + E group of ERβ group to inject antagonists of ERβ (PHTPP, HY-103456, MedChemExpress) into POA, and five rats from OVX group were randomly selected to inject agonists of ERβ (Liquiritigenin, HY-N0377, MedChemExpress) into POA. Other rats were given the same volume of cosolvent (10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline, MedChemExpress) in POA. After 72 h of drug injection, the POA was removed according to the above method, and the mRNA and protein expression of Vglut2 and Vgat in POA were detected by Western blot and qRT-PCR, respectively. The details on the primary and secondary antibodies of vglut2 and vgat are shown in Supplementary Table 1.1, and the details on the primers of vglut2 and vgat are shown in Supplementary Table 1.2.
Sun Y., Wang H., Wang W., Lu J., Zhang J., Luo X., Luan L., Wang K., Jia J., Yan J, & Qin L. (2022). Glutamatergic and GABAergic neurons in the preoptic area of the hypothalamus play key roles in menopausal hot flashes. Frontiers in Aging Neuroscience, 14, 993955.
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10

Topical LY3023414 Dose Evaluation

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To determine the topical dose of LY3023414 (Cat. No.: HY-12513, MedChem Express, Monmouth Junction, NJ, USA), four different concentrations, 0.5, 1.0, 2.5, and 5.0%, of LY3023414, dissolved in polyethylene glycol (PEG-300, Cat. No. 202371, Sigma Aldrich, Saint Louis, MO, USA), were applied topically to the anus of mice (N=3 per group) for two consecutive weeks, five days a week. 1% LY3023414 was the lowest concentration that resulted in a decrease in pAKT and pS6 expression, markers of the PI3K and mTOR activity respectively, as assessed by immunohistochemical staining (data not shown). Subsequently, all mice receiving topical LY3023414 were treated five days a week, Monday-Friday, topically at the anus with 20 μL of 1% LY3023414 in PEG-300.
Gunder L.C., Moyer T.H., Ziolkowski M.R., Keating M.K., Leverson G.E., Zhang W, & Carchman E.H. (2022). Systemic Delivery of a Dual PI3K/mTOR Inhibitor More Effective than Topical Delivery in Preventing Anal Carcinogenesis in an HPV Transgenic Mouse Model. Journal of cancer science and clinical therapeutics, 6(2), 157-173.
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