Mouse albumin elisa kit

To assess AFP and albumin secretion, the Mouse alpha Fetoprotein ELISA Kit (Abcam) and the Mouse Albumin ELISA kit (AssayPro), respectively, were used according to the manufacturers' instructions (Table S2, part 2). Supernatants were collected 24 h following the most recent medium change.

Renal function was assessed by determination of creatinine clearance. Therefore creatinine in serum and urine was measured according to standard procedures using a Cobas analyzer (Roche, Mannheim, Germany) in the Institute of Clinical Biochemistry and Pathobiochemistry of the University of Würzburg. For quantification of albumin excretion, the Mouse Albumin ELISA Kit (EMA 3201-1, Assay Pro, St. Charles, USA) was used according to the manufacturer's protocol. The absorbance was read at 450 nm with an ELISA reader (Spectra MAX 340, Molecular Devices, Ismaning, Germany) and a standard curve was generated on each plate.

Renal function was assessed by measuring serum creatinine, calculating creatinine clearance and quantifying excretion of albumin, kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL). Creatinine in urine and serum was determined using creatinine urinary/serum colorimetric assay kits (No. 500701/700460, Cayman Chemical Company, Ann Arbor, MI, USA) according to the manufacturer’s protocol. The mouse albumin ELISA kit (EMA3201-1, Assay Pro, St. Charles, IL, USA), the mouse KIM1/TIM1 ELISA kit (PicoKineTM, EK0880, BosterBio, Pleasanton, CA, USA) and the mouse NGAL ELISA kit (KIT 042, BioPorto, Gentofte, Denmark) were used for the quantification of albumin, KIM-1 and NGAL excretion following the manufacturer’s protocol. Albumin, KIM-1 and NGAL were related to urinary creatinine.