Horseradish peroxidase hrp conjugated horse anti mouse igg

Manufactured by Cell Signaling Technology

Sourced in United States

Horseradish peroxidase (HRP)-conjugated horse anti-mouse IgG is a secondary antibody used in immunoassays. It is composed of HRP, an enzyme, conjugated to horse-derived antibodies that specifically recognize mouse immunoglobulin G (IgG).

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3 protocols using horseradish peroxidase hrp conjugated horse anti mouse igg

Acetylated Glutathione and ADH Assay

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S-Acetyl glutathione (Product# AMBH95E07091) and alcohol dehydrogenase (ADH) (Product# A7011) were obtained from Sigma Aldrich (St. Louis, MO, USA). Monoclonal antibodies against acetyllysine (AcK) (Cat# 9681S) and horseradish peroxidase (HRP)-conjugated horse anti-mouse IgG (Cat# 7076S) were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were of analytical grade.

Panja S., Gaikwad H., Rankenberg J., Nam M.H, & Nagaraj R.H. (2023). Promotion of Protein Solubility and Reduction in Stiffness in Human Lenses by Aggrelyte-1: Implications for Reversing Presbyopia. International Journal of Molecular Sciences, 24(3), 2196.

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Comprehensive Antibody Panel for Extracellular Vesicle Characterization

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The primary antibodies used in this study were as follows: anti-CD63 (dilution 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-CD81 (dilution 1:1000, MBL, Nagoya, Japan), anti-TSG101 (dilution 1:1000, Abcam, Cambridge, MA), anti-flotillin-1 (dilution 1:1000, Cell Signaling Technology, Danvers, MA), anti-actinin-4 (dilution 1:1000, Cell Signaling Technology), anti-β-actin (dilution 1:5000, Sigma-Aldrich), anti-CLDN11 (dilution 1:1000, R&D Systems, Minneapolis, MN), anti-vimentin (dilution 1:1000 in western blotting and 1:100 in immunocytochemistry, Cell Signaling Technology), anti-ZO-1 (dilution 1:1000, Cell Signaling Technology), and anti-Snail (dilution 1:1000, Cell Signaling Technology). The secondary antibodies used in this study were as follows: horseradish peroxidase (HRP)-conjugated horse anti-mouse IgG (dilution 1:1000, Cell Signaling Technology), HRP-conjugated goat anti-rabbit IgG (dilution 1:1000, Cell Signaling Technology), and Alexa Fluor 488-conjugated goat anti-rabbit IgG (dilution 1:1000, Thermo Fisher Scientific).

Yamada N.O., Heishima K., Akao Y, & Senda T. (2019). Extracellular Vesicles Containing MicroRNA-92a-3p Facilitate Partial Endothelial-Mesenchymal Transition and Angiogenesis in Endothelial Cells. International Journal of Molecular Sciences, 20(18), 4406.

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Antibodies for Cellular Signaling Analysis

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Anti-HIF-1α antibody (610959, 1:1000) was obtained from BD (San Jose, CA, USA). Anti-α-SMA (A5228, 1:2000), anti-vimentin (V2258, 1:4000for WB and 1:1000 for IF), andanti-acetyl-histone H3 (06–599, 1:1000) antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-E-cadherin (3195S, 1:1000 for WB and 1:200 for IF), anti-HADC3 (85057S, 1:1000), anti-β-actin (58169Sor 4970S, 1:2000), and horseradish peroxidase (HRP)-conjugated horse anti-mouse IgG (7076, 1:2000) and goat anti-rabbit IgG (7074, 1:2000) were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-rabbit IgG (Alexa Fluor^® 488) (A-11008, 1:500), anti-mouse IgG (Alexa Fluor^® 594) (A32742, 1:500), Lipofectamine^™ 2000 Reagent (11668–019), Lipofectamine^®RNAiMAXReagent (13778–030),Silencer^™ Select HIF-1α siRNA (4390824, assay ID s6541),and Negative Control No. 2 siRNA (4390846) were purchased from Invitrogen (Carlsbad, CA, USA).HDAC3 siRNA (sc-35538) and negative siRNA control (sc-37007) were from Santa CruzBiotechnology (CA, USA). Other chemicals were obtained from Fisher (Pittsburgh, PA, USA)unless otherwise indicated.

Yuan J., Mo Y., Zhang Y., Zhang Y, & Zhang Q. (2022). Nickel nanoparticles induce epithelial-mesenchymal transition in human bronchial epithelial cells via the HIF-1α/HDAC3 pathway. Nanotoxicology, 16(6-8), 695-712.

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