Spm data processing software

Manufactured by Bruker

The SPM Data Processing software is a tool designed for the analysis and processing of data acquired by Scanning Probe Microscopy (SPM) instruments. It provides a comprehensive set of functionalities for the visualization, processing, and analysis of SPM data, enabling users to effectively extract relevant information from their measurements.

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Lab products found in correlation

Ac240ts, by Oxford Instruments (2 mentions) Nanowizard ultra speed, by Bruker (1 mentions) Nanowizardultra speed microscope, by Bruker (1 mentions) Omcl ac160ts, by Olympus (1 mentions) Nanowizard 3, by Bruker (1 mentions) Dimension fastscan, by Bruker (1 mentions)

Close spelling variants of this product

Data Processing software (20 citations) SPM Data Processing (3 citations)

5 protocols using spm data processing software

High-Resolution Atomic Force Microscopy

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Atomic force microscopy (AFM)
images were obtained with a JPK NanoWizard Ultra Speed instrument,
and the images were processed on JPK SPM Data Processing software.
The experiments were performed using a silicon probe (AC240TS, Asylum
Research) with 300 kHz nominal resonance frequency. The images were
scanned in an intermittent contact mode at room temperature with 512
× 512 pixels.

Lima L.M., Arjmandi-Tash H, & Schneider G.F. (2018). Lateral Non-covalent Clamping of Graphene at the Edges Using a Lipid Scaffold. ACS Applied Materials & Interfaces, 10(13), 11328-11332.

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Atomic Force Microscopy of Elastin-Like Proteins

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Samples for AFM were prepared by drop-casting 20 µL of 37°C 2 µM ELP or ELP/ELP-Bet v 1 on a silicon oxide wafer (Siegert Wafer) with a 285 nm thermal oxide layer or on a mica disc (V1 grade; Muscovite). The samples were dried at 37°C for 30 minutes. AFM images were recorded using a JPK NanoWizard Ultra Speed microscope and the obtained data was processed using the JPK SPM Data Processing software. All experiments were performed using a silicon probe (Olympus, OMCL-AC160TS) with a nominal resonance frequency of 300 kHz. The images were all scanned and recorded (with a resolution of 512x512 pixels) in intermittent contact mode in air at room temperature.

van Strien J., Warmenhoven H., Logiantara A., Makurat M., Aglas L., Bethanis A., Leboux R., van Rijt L., MacKay J.A., van Schijndel J.W., Schneider G., Olsthoorn R., Jiskoot W., van Ree R, & Kros A. (2022). Bet v 1-displaying elastin-like polypeptide nanoparticles induce a strong humoral and weak CD4+ T-cell response against Bet v 1 in a murine immunogenicity model. Frontiers in Immunology, 13, 1006776.

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Atomic Force Microscopy for Spheroid Elasticity

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The DLD-1 spheroids apparent Young’s modulus (E, in Pa) was
calculated by fitting each recorded force-indentation curve with the Hertz
contact mechanics model for a rigid spherical probe indenting an infinite
isotropic elastic half-space:

F_{H e r t z} = \frac{4 E \sqrt{R} δ^{3 / 2}}{3 (1 - v^{2})}

In the above equation, F is the
applied force, E is the elastic Young’s
modulus, υ is the Poisson’s
ratio (assumed to be 0.5 for soft material), Ris the radius of the AFM probe (17.5 μm), and
δ is the spheroid indentation.
AFM data analysis was performed using the JPK SPM Data Processing software.
A 2 μm deformation range was selected from near the maximum
indentation to fit the Hertz model.

Wang S., Matsumoto K., Lish S.R., Cartagena-Rivera A.X, & Yamada K.M. (2021). Budding Epithelial Morphogenesis Driven by Cell-Matrix versus Cell-Cell Adhesion. Cell, 184(14), 3702-3716.e30.

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Patterned Surface Characterization by SEM and AFM

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Patterned surfaces were characterized by SEM using
a Mira3 scanning electron microscope and a VEGA microscope (TESCAN
Brno, s.r.o.) and by AFM using a NanoWizard 3 instrument (JPK Instruments)
and a Dimension FastScan instrument (Bruker) for high resolution.
For AFM, the scanning frequency was set at 1 kHz for a resolution
of 512 × 512 pixels and 0.5 kHz for a resolution of 1024 ×
1024 pixels. The final images were processed and analyzed with SPM
data processing software (JPK Instruments).

Pospíšil J., Hrabovský M., Bohačiaková D., Hovádková Z., Jurásek M., Mlčoušková J., Paruch K., Nevolová Š., Damborsky J., Hampl A, & Jaros J. (2022). Geometric Control of Cell Behavior by Biomolecule Nanodistribution. ACS Biomaterials Science & Engineering, 8(11), 4789-4806.

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Atomic Force Microscopy Imaging Protocols

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This 4.6. AFM microscopy AFM images were performed on a JPK NanoWizard Ultra Speed machine and the images were processed using a JPK SPM Data Processing software. The experiments were performed using a silicon probe (AC240TS, Asylum Research) with 70 kHz nominal resonance frequency. The images were scanned in an intermittent contact mode in air at room temperature with 512 × 512 pixels.

Lima L.M., Fu W., Jiang L., Kros A, & Schneider G.F. (2016). Graphene-stabilized lipid monolayer heterostructures: a novel biomembrane superstructure. Nanoscale, 8(44).

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