Fitc labeled goat anti mouse igg

Manufactured by Jackson ImmunoResearch

Sourced in United States, United Kingdom

FITC-labeled goat anti-mouse IgG is a secondary antibody that specifically binds to mouse immunoglobulin G (IgG) antibodies. The FITC (Fluorescein Isothiocyanate) label allows for fluorescent detection of the target mouse IgG.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 510 confocal laser scanning microscope, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by AAT Bioquest (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Dp bsw software, by Olympus (1 mentions) Bx51 fluorescent microscope, by Olympus (1 mentions)

8 protocols using fitc labeled goat anti mouse igg

Kidney Histology and Immunofluorescence Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue specimens were fixed in 10% neutral buffered formalin and
embedded in paraffin. Tissue sections were stained with periodic acid schiff
(PAS) and analyzed by light microscopy. The nephritis scoring system was adapted
from the International Society of Nephrology/Renal Pathology Society (ISN/RPS)
classification of human lupus nephritis. At least 40 glomeruli per mouse were
evaluated. The final score accounted for morphological pattern (mesangial,
capillary, membranous) and for the percentage of involved glomeruli.
Immunofluorescence analysis on frozen kidney sections was performed by staining
with FITC-labeled goat anti–mouse IgG (Jackson ImmunoResearch
Laboratories) and specimens were analyzed with a LSM 510 laser scanning confocal
microscope (Carl Zeiss, Inc.). Images were captured by Q capture software. Five
representative glomeruli per mouse were chosen and mean fluorescent intensity
(MFI) was calculated using ImageJ software.

Manni M., Gupta S., Ricker E., Chinenov Y., Park S.H., Shi M., Pannellini T., Jessberger R., Ivashkiv L, & Pernis A.B. (2018). Regulation of Age-associated B cells by IRF5 in systemic autoimmunity. Nature immunology, 19(4), 407-419.

+ Open protocol

+ Expand

PCV2 Immunoassay Protocol Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Porcine Circovirus Type 2 (PCV-2, T657 strain) FA substrate slides (Cat No. SLD-IFA-PCV2, Lot. P140313-001, VMRD, USA) were incubated with a 1:100 dilution of antiserum from experimentally peptide-immunized mice, and a 1:100 dilution of PCV2 convalescent-phase swine antiserum. After incubation at 37 °C for 1 h, slides were gently rinsed briefly in PBS and then soak for 15 min in PBS at 4 °C. The slides were then incubated at 37 °C with fluorescein isothiocyanate (FITC)-labeled goat anti-mouse IgG (subclasses 1 + 2a + 2b + 3, Fcγ), FITC- labeled goat anti-pig IgG (H + L) (Jackson Immunoresearch, West Grove, PA, USA). 30 min after incubation, slide were washed with PBS and then incubated with 4, 6-diamidino-2-phenylindole (DAPI) (AAT Bioquest, Sunnyvale, CA, USA) at a dilution of 1 in 2300 in PBS for 15 min at room temperature. Samples were mounted under 50% glycerol and observed with an Olympus BX51 fluorescence microscope and SPOT FlEX camera (Diagnostic Instrument, Model 15.2 64MP, USA).

Hung L.C., Yang C.Y, & Cheng I.C. (2017). Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies. BMC Immunology, 18, 25.

+ Open protocol

+ Expand

ErbB2 Expression Quantification in HNC

Check if the same lab product or an alternative is used in the 5 most similar protocols

MAb 4D5 was used to detect the level of ErbB2 expression in human HNC cell lines with flow cytometric analysis using a FACS Calibur cytometer. SCC-15, FaDu, and A-253 cell tumor cells (5 × 10⁵/tube) were labeled with the primary antibody anti-human ErbB2 mAb 4D5 (5 µg/mL) and then with FITC-labeled goat anti-mouse IgG (1:100) (catalog #115-095-146, Jackson Immunoresearch, Ely, UK). MOPC-21 (5 μg/mL) was used as control. Antibody binding was analyzed using Flowing Software 2.5.1.

Focaccetti C., Benvenuto M., Ciuffa S., Fazi S., Scimeca M., Nardi A., Miele M.T., Battisti A., Bonanno E., Modesti A., Masuelli L, & Bei R. (2020). Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-neuT) in Mice with Transplanted Salivary Gland Carcinoma Cells. Nutrients, 12(5), 1417.

+ Open protocol

+ Expand

Kidney Cryosection Antibody Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kidney cryosections from Rag2^−/− mice of homozygous matings were incubated with supernatants or purified antibodies as described in Ref. (27 (link)). For detection, either FITC labeled goat anti-mouse IgG (Jackson ImmunoResearch) or FITC labeled rabbit anti-human IgG (Jackson ImmunoResearch) were used.

Faderl M., Klein F., Wirz O.F., Heiler S., Albertí-Servera L., Engdahl C., Andersson J, & Rolink A. (2018). Two Distinct Pathways in Mice Generate Antinuclear Antigen-Reactive B Cell Repertoires. Frontiers in Immunology, 9, 16.

+ Open protocol

+ Expand

Immunofluorescence Assay for Dengue Virus

Check if the same lab product or an alternative is used in the 5 most similar protocols

BHK-21 cells were cultured on slides in a 24-well plate overnight. The cells were infected with DENV1-4 at multiplicity of infection (MOI) of 1–10. After 48 hours, the infected cells were fixed with 1:1 methanol/acetone at -20°C for 10 minutes. The slides were washed with PBS, and then blocked with 1% bovine serum albumin (BSA) in PBS at RT for 30 minutes. Next, the infected cells were incubated with mAbs at RT for 1 hour. After washing with PBST_0.1, the slides were stained with fluorescent-isothiocyanate (FITC)-labeled goat anti-mouse IgG (Jackson ImmunoResearch Laboratories) and 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen) at RT for 1 hour. After washing, the slides were examined under a fluorescent microscope.

Tang C.T., Liao M.Y., Chiu C.Y., Shen W.F., Chiu C.Y., Cheng P.C., Chang G.J, & Wu H.C. (2015). Generation of Monoclonal Antibodies against Dengue Virus Type 4 and Identification of Enhancing Epitopes on Envelope Protein. PLoS ONE, 10(8), e0136328.

+ Open protocol

+ Expand

Histological and Immunofluorescence Analysis of Murine Lupus Nephritis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue specimens were fixed in 10% neutral buffered formalin and embedded in paraffin. Tissue sections were stained with periodic acid-Schiff (PAS) or with hematoxylin and eosin (H&E) and analyzed by light microscopy. The nephritis scoring system was adapted from the International Society of Nephrology/Renal Pathology Society (ISN/RPS) classification of human lupus nephritis. The final score accounted for morphological pattern (mesangial, capillary, membranous) and for the percentage of involved glomeruli. For immunofluorescence staining, kidneys or spleens were embedded in OCT and frozen in 2-methylbutane surrounded by dry ice. Frozen blocks were cut into 9 μm section with cryotome and stored at −80 ^oC. Upon thawing, sections were let dry at room temperature and stained. Spleen sections were stained with B220 (BD; RA3-6B2) and GL7 (BioLegend). Kidney sections were stained with FITC-labeled goat anti-mouse IgG (Jackson ImmunoResearch). Specimens were captured by Q capture software on a Nikon Eclipse microscope and quantifications were calculated using ImageJ software.

Ricker E., Manni M., Flores-Castro D., Jenkins D., Gupta S., Rivera-Correa J., Meng W., Rosenfeld A.M., Pannellini T., Bachu M., Chinenov Y., Sculco P.K., Jessberger R., Prak E.T, & Pernis A.B. (2021). Altered function and differentiation of age-associated B cells contribute to the female bias in lupus mice. Nature Communications, 12, 4813.

+ Open protocol

+ Expand

Detecting Mouse IgG ANA by HEp-2 Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kallestad HEp-2 (Bio-Rad, catalog no. 30472) was used to detect ANA staining according to the manufacturer’s instruction, modified to detect mouse IgG. Briefly, wells were incubated with 40 μl of mouse serum, diluted 40× in PBS with 1% BSA, at RT for 20 min. After incubation, slides were washed in PBS for 10 min and incubated with 30 μl of FITC-labeled goat anti-mouse IgG (Jackson ImmunoResearch, catalog no. 115-095-164) diluted 200× in DAPI for 20 min at RT, followed by a 10-min PBS wash. Slides were then mounted and coverslipped and viewed on an Olympus BX51 fluorescent microscope equipped with a digital camera and DP-BSW software (Olympus). Images were quantified, pseudo-colored, and merged using ImageJ software (NIH) with the Fiji plugin.

Brian BF I.V., Sauer M.L., Greene J.T., Senevirathne S.E., Lindstedt A.J., Funk O.L., Ruis B.L., Ramirez L.A., Auger J.L., Swanson W.L., Nunez M.G., Moriarity B.S., Lowell C.A., Binstadt B.A, & Freedman T.S. (2022). A dominant function of LynB kinase in preventing autoimmunity. Science Advances, 8(16), eabj5227.

+ Open protocol

+ Expand

Histological and Immunofluorescence Analysis of Kidney and Spleen

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue specimens were fixed in 10% neutral buffered formalin and embedded in paraffin. Tissue sections were stained with periodic acid-Schiff (PAS) or with hematoxylin and eosin (H&E) and analyzed by light microscopy. The nephritis scoring system was adapted from the International Society of Nephrology/Renal Pathology Society (ISN/RPS) classification of human lupus nephritis. The final score accounted for morphological pattern (mesangial, capillary, membranous) and for the percentage of involved glomeruli. For immunofluorescence staining, kidneys or spleens were embedded in OCT and frozen in 2-methylbutane surrounded by dry ice.
Frozen blocks were cut into 9µm section with cryotome and stored at -80 o C. Upon thawing, sections were let dry at room temperature and stained. Spleen sections were stained with B220 (BD; RA3-6B2) and GL7 (BioLegend). Kidney sections were stained with FITC-labeled goat antimouse IgG (Jackson ImmunoResearch). Specimens were captured by Q capture software on a Nikon Eclipse microscope and quantifications were calculated using ImageJ software.

Ricker E., Manni M., Flores-Castro D., Jenkins D.E., Gupta S., Rivera‐Correa J., Meng W., Rosenfeld A.M., Pannellini T., Bachu M., Chinenov Y., Sculco P.K., Jessberger R., Prak E.T.L., & Pernis A.B. (2021). Sex-specific differences in the function and differentiation of ABCs mark TLR7-driven immunopathogenesis.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!