Ethanol

Manufactured by Lach-Ner

Sourced in Czechia

Ethanol is a clear, colorless liquid that is used as a laboratory reagent. It serves as a solvent, disinfectant, and fuel source. Ethanol is produced through the fermentation of sugars and can be used in various applications within a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using ethanol

Synthesis and Characterization of Copolymer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Styrene, maleic anhydride (MA), p-divinylbenzene (DVB) and 2,2’-azo-bis(isobutyronitrile) (AIBN) were purchased from Sigma-Aldrich (Prague, Czech Republic). Styrene was distilled, MA was resublimed and AIBN was recrystallized from methanol prior to use. Ethyl acetate, ethanol and acetone of analytical grade were obtained from Lach-Ner (Neratovice, Czech Republic). Acetonitrile (ACN) and water of HPLC grade were purchased from VWR (Prague, Czech Republic). Phosphate buffered saline (PBS) solution (pH 7.4) was prepared from tablets supplied by Sigma-Aldrich (Prague, Czech Republic) according to the manufacturer’s instructions. Fetal bovine serum (FBS) was purchased from Sigma-Aldrich (Prague, Czech Republic). Cell culture medium (CellGRO) and AlamarBlue Assay were purchased from ThermoFisher Scientific (Prague, Czech Republic), 24-well plates were from TPP (Prague, Czech Republic).

Hrib J., Chylikova Krumbholcova E., Duskova-Smrckova M., Hobzova R., Sirc J., Hruby M., Michalek J., Hodan J., Lesny P, & Smucler R. (2019). Hydrogel Tissue Expanders for Stomatology. Part II. Poly(styrene-maleic anhydride) Hydrogels. Polymers, 11(7), 1087.

+ Open protocol

+ Expand

Ion-Molecule Reactions of VOCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

A wide scope of chemical
classes of VOCs
were included in order to ascertain the differences in ion–molecule
reactions between the literature data and the present data obtained
from the two instruments. We therefore used 1-propanol (PENTA, 99.5%),
2-propanol (PENTA, 99.8%), 2,3-butanedione (Aldrich, 97%), acetaldehyde
(Sigma-Aldrich, ≥99.5%), acetic acid (Sigma-Aldrich, ≥99.7%),
acetone (PENTA, 99.5%), ethyl acetate (Sigma-Aldrich, 99.8%), ethanol
(Lachner, 96%), and R-limonene (Sigma-Aldrich, Analytical
Standard).

Swift S.J., Španěl P., Sixtová N, & Demarais N. (2023). How to Use Ion-Molecule Reaction Data Previously Obtained in Helium at 300 K in the New Generation of Selected Ion Flow Tube Mass Spectrometry Instruments Operating in Nitrogen at 393 K. Analytical Chemistry, 95(29), 11157-11163.

+ Open protocol

+ Expand

Synthesis and Characterization of Silica Nanoparticles for Immunological Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

FeCl₂ · 4H₂O and FeCl₃ · 6H₂O were purchased from Fluka (Buchs, Switzerland). Sodium hypochlorite solution (5 wt.%) was from Bochemie (Bohumín, Czech Republic). Tetramethyl orthosilicate (TMOS), (3-aminopropyl)triethoxysilane (APTES), (3-aminopropyl)dimethylethoxysilane (APDMES), RPMI-1640 medium, phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), L-glutamine, and hydroethidine were purchased from Sigma-Aldrich (Steinheim, Germany). Anti-CD3 monoclonal antibody was purchased from Beckman Coulter and fetal calf serum (FCS) from PAA. Fluorescein-labeled Staphylococcus aureus bacteria and opsonizing reagent were purchased from Molecular Probes (Eugene, OR, USA). Cationic surfactant, cetyltrimethylammonium bromide (CTAB) was purchased from Lachema (Brno, Czech Republic). Ammonium hydroxide solution (25%) and ethanol were purchased from Lach-Ner (Neratovice, Czech Republic). Gentamycin was purchased from Sandoz (Bratislava, Slovakia), cyclophosphamide (Cyf) from Baxter (Deerfield, IL, USA), and [³H]-thymidine from Moravek Biochemicals (Brea, CA, USA). Scintillation fluid was purchased from Perkin Elmer (Waltham, MA, USA). Ultrapure Q-water ultrafiltered on a Milli-Q Gradient A10 system (Millipore, Molsheim, France) was used for preparation of the solutions. ELISA sets were purchased from Affymetrix e-Biosciences.

Zasońska B.A., Líšková A., Kuricová M., Tulinská J., Pop-Georgievski O., Čiampor F., Vávra I., Dušinská M., Ilavská S., Horváthová M, & Horák D. (2016). Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis, and immunotoxicity. Croatian Medical Journal, 57(2), 165-178.

+ Open protocol

+ Expand

Deuterated Estradiol Metabolism in Rat

Check if the same lab product or an alternative is used in the 5 most similar protocols

Commercial capacitating M2 culture media (M7167), deuterated β-estradiol-16,16,17-d₃ (E2-d₃) (purity 98%), acetonitrile (ACN) for LC-MS, Chromasolv (purity ≥ 99.9%), and phosphate buffered saline (PBS) tablets were purchased from Sigma-Aldrich^® (Steinheim, Germany). Formic acid (HCOOH) (LC-MS LiChropur, purity 97.5–98.5%), 17α-estradiol (purity 98%) and deionized water (for UHPLC-MS LiChrosolv) were provided by Merck (Darmstadt, Germany). Ethanol (purity ≥ 96% p.a.) was purchased from Lachner (Neratovice, Czech Republic). Paraffin oil was purchased from Carl Roth^® (Karlsruhe, Germany).
The experimental animals were laboratory inbred house rat strain Wistar (Velaz, Czech Republic). The males (n = 3) used for the experiments had reached sexual maturity and were 12 weeks old. The animals were housed in the animal facility of the Institute of Molecular Genetics of the Czech Academy of Science, Prague. Food and water were supplied ad libitum. All animal procedures and all experimental protocols were approved by the Animal Welfare Committee of the Czech Academy of Sciences (Animal Ethics Number 66866/2015-MZE-17214, 18 December 2015).

Bosakova T., Tockstein A., Bosakova Z, & Komrskova K. (2022). Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation. Molecules, 27(13), 4092.

+ Open protocol

+ Expand

Synthesis and Characterization of PEG-Neridronate

Check if the same lab product or an alternative is used in the 5 most similar protocols

FeCl₂ · 4H₂O, FeCl₃ · 6H₂O, Ce(NO₃)₃ · 6H₂O, trisodium citrate dihydrate, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). NH₄OH (25 wt.%), HCl (35 wt.%), H₂O₂ (30 wt.%), ethanol (96 %), propan-2-ol, and all other chemicals were purchased from LachNer (Neratovice, Czechia). Poly(ethylene glycol)-neridronate (PEG-Ner) was synthesized using PEG at a molecular weight of 2,000 or 5,000 Da as described previously (Kostiv et al., 2017 (link)).

Moskvin M., Marková I., Malínská H., Miklánková D., Hüttl M., Oliyarnyk O., Pop-Georgievski O., Zhigunov A., Petrovský E, & Horák D. (2020). Cerium Oxide-Decorated γ-Fe2O3 Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress. Frontiers in Chemistry, 8, 682.

+ Open protocol

+ Expand

Lanthanide-based Contrast Agents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chloride hexahydrates of terbium(III), neodymium(III), ytterbium(III), and gadoli-nium(III) (99.99%), sodium tetrafluoroborate, phosphate-buffered saline (PBS), and N,N’-bis(2,3-dihydroxypropyl)-5-[N-(2,3-dihydroxypropyl)acetamido]-2,4,6-triiodoisophthal-amide (Iohexol) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ethanol (99%), ethylene glycol (EG), acetic and hydrochloric acids were obtained from Lach-Ner (Neratovice, Czech Republic). Sodium hydroxide and sodium chloride were purchased from Lachema (Brno, Czech Republic). Sodium acetate (NaAc) buffer (pH 5) was prepared from sodium hydroxide and acetic acid. Gibco™ penicillin-streptomycin, CellMask™ deep red and PrestoBlue™ cell viability reagents were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Sulfo-Cyanine7 NHS ester (Cy7) was purchased from Lumiprobe (Hannover, Germany). Isoflurane was obtained from Baxter (San Juan, Puerto Rico). Alendronate-modified poly(N,N-dimethylacrylamide-co-N-acryloylglycine methyl ester) [P(DMA-AGME)-Ale; M_w = 11,000 g/mol] (Figure 1) was synthesized according to previously published procedure [29 (link)]. All chemicals were used directly without further purification. Ultrapure Q-water ultra-filtered on a Milli-Q Gradient A10 system (Millipore; Molsheim, France) was used in all experiments.

Shapoval O., Oleksa V., Šlouf M., Lobaz V., Trhlíková O., Filipová M., Janoušková O., Engstová H., Pankrác J., Modrý A., Herynek V., Ježek P., Šefc L, & Horák D. (2021). Colloidally Stable P(DMA-AGME)-Ale-Coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ Nanoparticles as a Multimodal Contrast Agent for Down- and Upconversion Luminescence, Magnetic Resonance Imaging, and Computed Tomography. Nanomaterials, 11(1), 230.

+ Open protocol

+ Expand

Photoactive Hydrogel Biomaterial Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium selenite (Na₂SeO₃), HEMA, and SMCA were purchased from Sigma Aldrich (Germany) and used as received. Potato starch was obtained from Agrana (Austria). Diphenyl (2,4,6-trimethylbenzoyl)-phosphine oxide (TPO) photoinitiator was obtained from RAHN (Switzerland). CRODA (France) kindly provided the Tween 20 surfactant. Acetone, ethanol, isopropanol and monochloroacetatic acid were purchased from Lach-ner (Czech Republic) and used as received.

Vishakha V., Abdel-Mohsen A.M., Michalicka J., White P.B., Lepcio P., Tinoco Navarro L.K, & Jančář J. (2023). Carboxymethyl starch as a reducing and capping agent in the hydrothermal synthesis of selenium nanostructures for use with three-dimensional-printed hydrogel carriers. Royal Society Open Science, 10(10), 230829.

+ Open protocol

+ Expand

Synthesis and Purification of Quercetin Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Iron (III) chloride hexahydrate (97%) was purchased from Alfa Easar (Ottawa, ON, Canada). Ammonium acetate and poly(Ethylene glycol) (PEG, Mw = 4000 Da) were purchased from Sigma Aldrich (St. Louis, MO, USA). Ethylene glycol and ethanol (96%) were purchased from Lach-ner (Neratovice, Czech Republic). Compressed nitrogen was purchased from Messer (Bad Soden am Taunus, Germany). Silicon oil was received from Acros organics (Waltham, MA, USA). Quercetin (≥99%) was supplied by Lach-ner (Neratovice, Czech Republic). 2-Propanol was purchased from Lach-ner (Neratovice, Czech Republic). Dialysis bags with molecular weight cut-offs (MWCOs) of 8 kDa and 25 kDa were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Mandić L., Matković M., Baranović G, & Šegota S. (2023). The Increased Release Kinetics of Quercetin from Superparamagnetic Nanocarriers in Dialysis. Antioxidants, 12(3), 732.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!