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Yolk-Gal4 is a genetic tool used in Drosophila research. It is a promoter construct that drives the expression of the Gal4 transcriptional activator specifically in the yolk cells of the Drosophila embryo.

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Lab products found in correlation

Tub gal4, by Bloomington Drosophila Stock Center (1 mentions) Fb gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas toll10b, by Bloomington Drosophila Stock Center (1 mentions) Uas tk rnai, by Bloomington Drosophila Stock Center (1 mentions) 24b gal4, by Bloomington Drosophila Stock Center (1 mentions) Ilp2 gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas upd2 rnai, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using yolk gal4

1

Drosophila Lipid Metabolism Regulation

Cited 2 times
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The following fly lines were used: w1118 (background control), yw (background control), FB-Gal4 (Schmid et al., 2014 (link)), tub-Gal4 (Bloomington Stock Center no. 5138), yolk-Gal4 (Bloomington Stock Center no. 58814), UAS-LipinRNAi (VDRC transformant ID 36007), UAS-rel (Bloomington Stock Center no.9459), UAS-Toll10b (Bloomington Stock Center no. 58987), plin138 (Bi et al., 2012 (link)), UAS-plin1 (Bi et al., 2012 (link)), UAS-TK RNAi (Bloomington Stock Center no. 25800), UAS-wRNAi (Bloomington Stock Center no. 28980), TKg-Gal4 (Song et al., 2014 (link)), PGRP-LE112 (Bloomington Stock Center no. 33055), PGRP-LCΔE (Bloomington Stock Center no. 55713). Genetic recombination was used to generate UAS-plin1; tub-Gal4.
Harsh S., Heryanto C, & Eleftherianos I. (2019). Intestinal lipid droplets as novel mediators of host–pathogen interaction in Drosophila. Biology Open, 8(7), bio039040.
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2

Drosophila Starvation Response Assay

Cited 1 time
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Flies were raised on standard agar-yeast-based food at 25°C, 50%–60% humidity with 12-hour light: 12-hour dark cycle. Starvation experiments were performed on 1% agar food for 24 hours. upd2Δ3–62 was kindly provided by J. Hombria [33 (link)]. The following stocks were from Bloomington Drosophila Stock Center (BDSC), yw (#1495), yolk-GAL4 (#58814), UAS-upd2RNAi (#33988, HMS00901) [110 (link)], UAS-domeRNAi (#32860), Ilp2-Gal4 (#37516), 24B-GAL4 (#1757), R57C10-GAL4 (#39171), R38H02-GAL4 (#47352) [111 (link)], UAS-CaLexa (#66542) [65 (link)]. Flies were crossed into a w+ (Canton-S) background (lab stock, originally obtained from BDSC) for all knockdown experiments.
Ertekin D., Kirszenblat L., Faville R, & van Swinderen B. (2020). Down-regulation of a cytokine secreted from peripheral fat bodies improves visual attention while reducing sleep in Drosophila. PLoS Biology, 18(8), e3000548.
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