Biotin fasl clone mfl3 armenian hamster igg2

Manufactured by BD

Sourced in United States

Biotin-FasL (clone MFL3) Armenian hamster IgG2 is a laboratory reagent. It is a biotinylated monoclonal antibody that binds to the Fas ligand (FasL) protein.

Automatically generated - may contain errors

Lab products found in correlation

Streptavidin fitc, by BD (2 mentions) Pe cy5 labeled anti mouse cd11c clone n418 hamster igg, by BioLegend (2 mentions) Fitc labeled anti mouse cd3ε clone 145 2c11 hamster igg, by BioLegend (2 mentions) Fitc labeled anti mouse cd25 clone pc61 rat igg1λ, by BioLegend (2 mentions) Pe labeled anti mouse cd8a clone 53 6.7 rat igg2aκ, by BioLegend (2 mentions) Pe cy5 labeled anti mouse cd4 clone gk1.5 rat igg2bκ, by BioLegend (2 mentions) Pe labeled anti mouse nk1.1 clone pk136 mouse igg2aκ, by BD (2 mentions) Annexin 5 fitc, by BD (2 mentions)

2 protocols using biotin fasl clone mfl3 armenian hamster igg2

Flow Cytometry Analysis of Immune Cells

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Antibodies used in the flow cytometry analysis were as follows: fluorescein isothiocyanate (FITC)-labeled anti-mouse Ly-6G/Ly-6C (Gr-1; clone RB6-8C5) rat IgG2bκ, phycoerythrin (PE)-labeled anti-mouse F4/80 (clone CIA3-1) rat IgG2bκ, PE/Cy5-labeled anti-mouse CD11c (clone N418) hamster IgG, FITC-labeled anti-mouse CD3ε (clone 145–2C11) hamster IgG, PE/Cy5-labeled anti-mouse CD45R/B220 (clone RA3-6B2) rat IgG2aκ, FITC-labeled anti-mouse CD25 (clone PC61) rat IgG1λ, PE-labeled anti-mouse CD8a (clone 53–6.7) rat IgG2aκ, and PE/Cy5-labeled anti-mouse CD4 (clone GK1.5) rat IgG2bκ were from BioLegend, Inc. (San Diego, CA, USA). PE-labeled anti-mouse NK1.1 (clone PK136) mouse IgG2aκ, biotin-FasL (clone MFL3) Armenian hamster IgG2, FITC-streptavidin and FITC-annexin V were from BD Pharmingen (San Diego, CA, USA). Monocytes/macrophages were defined as SSC^hiF4/80^hiGr-1lo population, granulocytes as SSC^hiGr-1^hi cells and dendritic cells as SSC^loF4/80-cells. B cells, T cells, NK cells, NKT cells were defined as SSC^lo CD45R+, SSC^loCD3ε+NK1.1-, SSC^loCD3ε+NK1.1+, and SSC^loCD3ε+NK1.1+, respectively. CD4 T cells (CD4+CD8-), CD8 T cells (CD4-CD8+), and regulatory T cells (CD4+CD8-CD25+) were also counted. Values represent the percentage of the non-parenchymal cells in each organ.

Uchida A., Yasuma T., Takeshita A., Toda M., Okano Y., Nishihama K., D’Alessandro-Gabazza C.N., Fridman D’Alessandro V., Inoue C., Takagi T., Mukaiyama H., Takagi N., Shimizu K., Yano Y, & Gabazza E.C. (2021). Oral Limonite Supplement Ameliorates Glucose Intolerance in Diabetic and Obese Mice. Journal of Inflammation Research, 14, 3089-3105.

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Multicolor Flow Cytometry Analysis of Immune Cells

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The percentage of spleen cells was evaluated by flow cytometry (BD Biosciences, Oxford, UK) using the following antibodies: fluorescein isothiocyanate (FITC)-labeled anti-mouse Ly-6G/Ly-6C (Gr-1; clone RB6-8C5) rat IgG2bκ, phycoerythrin (PE)-labeled anti-mouse F4/80 (clone CIA3-1) rat IgG2bκ, PE/Cy5-labeled anti-mouse CD11c (clone N418) hamster IgG, FITC-labeled anti-mouse CD3ε (clone 145-2C11) hamster IgG, PE/Cy5-labeled anti-mouse CD45R/B220 (clone RA3-6B2) rat IgG2aκ, FITC-labeled anti-mouse CD25 (clone PC61) rat IgG1λ, PE-labeled anti-mouse CD8a (clone 53-6.7) rat IgG2aκ, and PE/Cy5-labeled anti-mouse CD4 (clone GK1.5) rat IgG2bκ, which were from BioLegend, Inc. (San Diego, CA). PE-labeled anti-mouse NK1.1 (clone PK136) mouse IgG2aκ, biotin-FasL (clone MFL3) Armenian hamster IgG2, FITC-streptavidin, and FITC-annexin V were from BD Pharmingen. Monocytes/macrophages were defined as SSC^hiF4/80^hiGr-1lo population, granulocytes as SSC^hiGr-1^hi cells, and dendritic cells as SSC^loF4/80-cells. B cells, T cells, NK cells, and NKT cells were defined as SSC^lo CD45R+, SSC^loCD3ε+NK1.1-, SSC^loCD3ε+NK1.1+, and SSC^loCD3ε+NK1.1+, respectively. CD4 T cells (CD4+CD8-), CD8 T cells (CD4-CD8+), and regulatory T cells (CD4+CD8-CD25+) were also counted. Values represent the percentage of the non-parenchymal cells in each organ.

Yasuma T., Toda M., Abdel-Hamid A.M., D’Alessandro-Gabazza C., Kobayashi T., Nishihama K., D’Alessandro V.F., Pereira G.V., Mackie R.I., Gabazza E.C, & Cann I. (2021). Degradation Products of Complex Arabinoxylans by Bacteroides intestinalis Enhance the Host Immune Response. Microorganisms, 9(6), 1126.

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