The parasites were washed three times with phosphate buffered saline (PBS) pH 7.4. The samples were centrifuged at 2000 × g for 5 min. The pelleted cells were fixed with 4% glutaraldehyde and post-fixed with 1% osmium tetroxide. The specimens were then mounted on polycarbonate membrane (Nuclipore, Agar Scientific, USA) and dehydrated in increasing concentrations of ethanol (30%, 50%, 70%, 80%, 90% and 100%). The specimens were critical-point dried with carbon dioxide coated with gold, and examined with a scanning electron microscope (FEI-Quanta 200 FESEM, USA).
Fei quanta 200 fesem
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FEI-Quanta 200 FESEM is a field emission scanning electron microscope (FESEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a Schottky field emission gun that provides high-brightness electron beams, enabling high-resolution imaging and analytical capabilities. The instrument can operate in high-vacuum, low-vacuum, and environmental modes, allowing for the examination of a variety of sample types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fei quanta 200 fesem
1
Scanning Electron Microscopy of Parasites
2
Morphological Analysis via FESEM
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An Hitachi SU8220 (FEI Quanta 200 FESEM, FEI Company, Hillsboro, OR, USA) was used to conduct FESEM at a magnification of 500× to study the film’s morphology.
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