Cobas ampliprep cobas taqman hiv 1 test version

The copy number of virion-associated HIV RNA in the cell culture supernatants was measured using the Cobas Ampliprep/Cobas Taqman HIV-1 Test, Version 2.0 (Roche Diagnostics) following 36–48 hours of incubation of cells with the above study compounds. The limit of detection was 20 copies/mL.

We quantified HIV virions from plasma- and MNP-captured fractions using the Cobas Ampliprep/Cobas Taqman HIV-1 Test, Version 2.0 (Roche Diagnostics). The percentages of 2G12/PG9-captured, CD36⁺, and CD27⁺ virions in plasma were calculated as a ratio of captured and total plasma virion-associated HIV RNA.

Ethical approval for this study was obtained from New York University School of Medicine Institutional Review Board (10–02586) and Kenyatta National Hospital / University of Nairobi Ethics and Research Committee (P283/07/2011). Written informed consent and verbal assent when appropriate was obtained from all participants/parents. Individuals with a recent acute illness, fever, active Mycobaterium tuberculosis or malaria infection, or pregnancy within one year were not eligible for study entry. Plasma and peripheral blood mononuclear cells (PBMC) were isolated from peripheral blood by centrifugation and Ficoll-Hypaque (GE Healthcare) density gradient centrifugation then cryopreserved in -80°C and liquid nitrogen respectively. HIV RNA PCR was performed on diluted plasma samples with Roche, COBAS^® AmpliPrep/COBAS^® TaqMan^®HIV-1 Test, version 2.0 (limit of detection 110 copies/ml).