The primary antibodies used were: rabbit anti-CKIIα (2656), rabbit anti-BAF60c (62265), and rabbit anti-phospho-histone H3 (Ser10; D2C8) conjugated to Alexa Fluor 647 and were obtained from Cell Signaling Technologies. The rabbit anti-Brg1 (G-7), rabbit anti-BAF155 antibody (H-76), rabbit anti-lamin β1 (H-90), and rabbit anti-pol II (N-20) were purchased from Santa Cruz Biotechnology. The rabbit anti-PI3K p85 antibody, N-SH2 domain (ABS233), was obtained from Millipore Corp. The rabbit anti-Ini1 (BAF47) antisera was previously described [29 (link)]. The rabbit anti-SMARCE1 (BAF57, A13353) and rabbit anti-SMARC1 (BAF60a, A6310) were obtained from Abclonal Technology. The secondary anti-mouse and anti-rabbit horseradish peroxidase-conjugated antibodies and the Alexa Fluor 488- and 555-conjugated antibodies were from Thermo Fisher Scientific.
Rabbit anti pol 2 n 20
Manufactured by Santa Cruz Biotechnology
Rabbit anti-pol II (N-20) is a primary antibody that recognizes the N-terminus of the large subunit of RNA polymerase II, a key enzyme involved in the transcription of DNA into RNA. This antibody can be used for various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the localization and expression of RNA polymerase II.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 and 555 conjugated antibodies, by Thermo Fisher Scientific (1 mentions)
Rabbit anti phospho histone h3 ser 10 d2c8, by Cell Signaling Technology (1 mentions)
Rabbit anti myod c 20, by Santa Cruz Biotechnology (1 mentions)
Mouse anti gapdh peroxidase conjugated g9295, by Merck Group (1 mentions)
2 protocols using rabbit anti pol 2 n 20
1
Chromatin Remodeling Complex Antibody Panel
2
Antibody Characterization and Validation
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The antibodies used included the polyclonal rabbit antisera against Brg1 that was previously described (40 (link)). The rabbit anti-casein kinase IIα (H-286), mouse anti-casein kinase IIα′ (D-7), mouse anti-casein kinase IIβ (6D5), goat anti-Pax-3/7 antibody (N-19), rabbit anti-caspase-3 antibody (H-277), rabbit anti-Baf155 antibody (H-76), rabbit anti-Baf170 antibody (H-116), rabbit anti-lamin β1 (H-90), rabbit anti-pol II (N-20), and rabbit anti-MyoD (C-20) were purchased from Santa Cruz Biotechnology, Inc. Rabbit anti-PI3K p85 antibody, N-SH2 domain (ABS233) was obtained from Millipore Corp. Mouse anti-GAPDH-peroxidase-conjugated (G9295) was obtained from Sigma. Anti-myosin heavy chain (MF20, deposited by D. A. Fischman) antibody was obtained as a hybridoma supernatant from the Developmental Studies Hybridoma Bank (University of Iowa). Donkey anti-rabbit Alexa-488 (A21206) fluorescent secondary antibody was purchased from Thermo Fisher Scientific. Secondary antibodies anti-mouse and anti-rabbit HRP-coupled secondary antibodies were obtained from Pierce.
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