Ab31930

Manufactured by Abcam

Ab31930 is a purified monoclonal antibody produced in mouse. The antibody is designed for use in various immunoassays.

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Lab products found in correlation

2 protocols using ab31930

Antibody Characterization for Cell Biology

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The following antibodies were used for Western blotting:

RIF1; Bethyl Laboratories; A300‐568A; rabbit polyclonal

GFP; Abcam; ab290; rabbit polyclonal

GFP; ChromoTek; rat monoclonal [3H9]

MCM4; Abcam, ab4459; rabbit polyclonal

p‐S40‐MCM2; Abcam; ab133243; rabbit monoclonal [EPR4170(2)]

p‐S53‐MCM2; Bethyl Laboratories; A300‐756A; rabbit polyclonal

PP1α; Santa Cruz Biotechnology; sc‐271762; mouse monoclonal [G‐4]

PP1β; Santa Cruz Biotechnology; sc‐373782; mouse monoclonal [C‐5]

PP1γ; Santa Cruz Biotechnology; sc‐6108; goat polyclonal

Tubulin; Santa Cruz Biotechnology; sc‐53030; rat monoclonal [YOL1/34]

Following antibodies were used for flow cytometric analysis of chromatin‐associated proteins at indicated dilutions:

Cdt1 antibody; Abcam ab202067, at 1/100 dilution

FLAG tag antibody [M2]; Sigma F‐1804, at 1/300 dilution

MCM3 antibody; Santa Cruz sc‐9850, at 1/300 dilution

ORC2 antibody; Abcam ab31930, at 1/200 dilution

Alexa Fluor 488‐conjugated anti‐mouse IgG (ab150109), at 1/2,000 dilution

Alexa Fluor 568‐conjugated anti‐mouse IgG (ab175700), at 1/2,000 dilution

Alexa Fluor 647‐conjugated anti‐goat IgG (ab150135), at 1/2,000 dilution

Alexa Fluor 647‐conjugated anti‐rabbit IgG (ab150063), at 1/2,000 dilution

For immunoprecipitation of endogenous RIF1 protein, Abcam ab70254 was used. For microscopic observation of Cdc6 protein, Abcam ab188423 was used.

Hiraga S., Ly T., Garzón J., Hořejší Z., Ohkubo Y., Endo A., Obuse C., Boulton S.J., Lamond A.I, & Donaldson A.D. (2017). Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Reports, 18(3), 403-419.

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Analysis of DNA Repair Proteins

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The following antibodies were used: mouse anti-BRCA1 (OP92, Calbiochem) 1:2000, rabbit anti-BRCA2 (ab123491, Abcam) 1:2000, rabbit anti-DCP1a (ab47811, Abcam) 1:1000, rabbit anti-EDC4 (ab72408, Abcam) 1:1000, rabbit anti-FANCA (A301-980A, Bethyl Laboratories) 1:1000, rabbit anti-FANCD2 (ab2187, Abcam) 1:2500, rabbit anti-BRIP1 (ab16608, Abcam) 1:1000, rabbit anti-GAPDH (ab9485, Abcam) 1:1000, rabbit anti-HA tag (ab9110, Abcam) 1:1000, mouse anti-ORC2 (ab31930, Abcam) 1:1000, rabbit anti-RAD51 (8349, Santa Cruz) 1:250, rabbit anti-TOPBP1 (ab2402, Abcam) 1:1000, mouse anti-vinculin (ab18058, Abcam) 1:5000, goat anti-rabbit-Alexa 568 conjugated (A11036, Molecular Probes) 1:500, goat anti-rabbit-Alexa 488 conjugated (A11034, Molecular Probes) 1:500 and goat anti-mouse-Alexa 568 conjugated (A11031, Molecular Probes) 1:500, goat anti-rabbit IgG-HRP conjugated (A120-201P, Bethyl Laboratories) 1:1000, and goat anti-mouse IgG-HRP conjugated (sc-2005, Santa Cruz) 1:1000.

Hernández G., Ramírez M.J., Minguillón J., Quiles P., Ruiz de Garibay G., Aza-Carmona M., Bogliolo M., Pujol R., Prados-Carvajal R., Fernández J., García N., López A., Gutiérrez-Enríquez S., Diez O., Benítez J., Salinas M., Teulé A., Brunet J., Radice P., Peterlongo P., Schindler D., Huertas P., Puente X.S., Lázaro C., Pujana M.À, & Surrallés J. (2018). Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1. Nature Communications, 9, 967.

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