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Litesizer dls 500

Manufactured by Anton Paar
Sourced in Austria

The Litesizer DLS 500 is a dynamic light scattering (DLS) instrument designed for the measurement of particle size and zeta potential. It utilizes laser light scattering technology to determine the hydrodynamic size of particles or molecules in suspension.

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2 protocols using litesizer dls 500

1

Characterization of Synthesized AgNPs

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The amount of 50 µL of a 1000 ppm vial containing the synthesized AgNPs that displayed the best remediation potential for each plant part were diluted in 950 µL of deionized water and were subjected to characterization through UV–vis spectroscopy (200–800 nm), EDS, SDL, XPS, and XRD. Based on the XRD results, the mean size of the crystallites from each sample of AgNPs was calculated using the Debye–Scherrer equation (Equation (1)) [57 (link)]:
The zeta potential (Litesizer DLS 500—Anton Paar; Graz, Austria) and TEM images (TecnaiTM G2 Spirit Biotwin 120 kV—FEI Company; Hillsboro, OR, USA) of the AgNPs were also obtained. Regarding the TEM analysis, the size of the particles present in the images were measured using ImageJ 1.54 g (ImageJ, Bethesda, MD, USA) to access the size distribution profile.
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2

Dynamic Light Scattering of NTS-Polyplex Nanoparticles

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The Litesizer DLS 500 (Anton Paar, Graz, Austria) was used to measure the size and charge of NTS-polyplex NPs harboring either the pTracer-rNurr1-V5 or the pEGFP-N1 prepared in DMEM without phenol red. First, the diameter distribution was determined by dynamic light scattering in 1 mL of NTS-polyplex NPs at RT (25°C, angle 90°) using a disposable cuvette (Sarstedt, Nümbrecht, Germany). Then, the zeta potential was measured in 50 μL of NTS-polyplex NPs using a Univette Low Volume and 4 V. The dataset was processed using the KalliopeTM Software (Anton Paar).
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