The largest database of trusted experimental protocols

Hy 100941

Manufactured by MedChemExpress
Sourced in United States

HY-100941 is a laboratory equipment product offered by MedChemExpress. It is designed for use in research and development settings. The core function of HY-100941 is to facilitate the handling and processing of samples in a controlled environment.

Automatically generated - may contain errors

3 protocols using hy 100941

1

Molecular Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pyrrolidinedithiocarbamate ammonium (PDTC, HY-18738) and carbonyl cyanide 3-chlorophenylhydrazone (CCCP, HY-100941) were purchased from MedChemExpress. Recombinant iIFN1a protein was generated in our previous study and stored at −80 °C [6 (link)]. Rabbit anti-WNT3a polyclonal antibody (D122111), rabbit anti-CCND1 polyclonal antibody (D160236), and HRP-conjugated goat anti-rabbit IgG were purchased from Sangon Biotech (Shanghai, China). Rabbit monoclonal antibodies against beta-tubulin were obtained from Abcam (Cambridge, UK; EPR16774).
+ Open protocol
+ Expand
2

Sal B Protects HUVECs from HG-Induced Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human umbilical vein endothelial cells (HUVECs; ATCC, USA) were grown in Dulbecco’s modified Eagle’s medium (Gibco, USA) plus 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 U/ml streptomycin. HUVECs were grown in an environment of 5% CO2 at 37°C. Diabetes cell model was constructed by 30 mM high-glucose (HG)-induced HUVECs. 0.1% DMSO was utilized for improving the solubility and bioavailability of Sal B. HUVECs were treated with 0, 5, 10, 30, 50, 80 and 100 μM Sal B for 48 h in HG-pre-treated HUVECs. Furthermore, HUVECs were treated with 50 nM oxidative phosphorylation uncoupler carbonyl cyanide m-chlorophenyl hydrazone (CCCP; HY-100941; Med Chem Express, USA) that was dissolved by 0.1% DMSO.
+ Open protocol
+ Expand
3

Morphine-Induced Autophagy Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Morphine was provided by Sun Yat-sen Memorial Hospital, which was approved by Guangdong Medical Products Administration. CCCP (10 μM, HY-100941), Mdivi-1 (20 μM, HY-15886) and Bafilomycin A1 (20 nM, HY-100558) were purchased from MedChemExpress. Torin1 (250 μM, SC0245) was purchased from Beyotime. Puromycin (Sigma, P7255) was used to select stably expressed cells.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!