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Pe conjugated cd105

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PE-conjugated CD105 is a cell surface marker protein. It is commonly used in flow cytometry applications to identify and analyze endothelial cells.

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Lab products found in correlation

Fitc conjugated cd29, by Thermo Fisher Scientific (2 mentions) Hla abc, by Thermo Fisher Scientific (2 mentions) Vimentin, by Santa Cruz Biotechnology (1 mentions) Apc conjugated cd31, by Thermo Fisher Scientific (1 mentions) Percp cy5.5 conjugated cd45, by Thermo Fisher Scientific (1 mentions) Collagenase, by Merck Group (1 mentions) Cd166, by Thermo Fisher Scientific (1 mentions) Pe conjugated cd144, by BD (1 mentions) Fitc conjugated cd14, by BD (1 mentions) Fitc conjugated annexin 5, by BD (1 mentions) Cytomics fc500 mpl cytometer, by Beckman Coulter (1 mentions) Hla dr, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD105 (115 citations) CD105-PE (51 citations) PE-conjugated mouse anti-human CD105 (4 citations)

4 protocols using pe conjugated cd105

1

Multiparameter Flow Cytometry Panel

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Antibodies were purchased from the following sources: PE conjugated CD105, FITC conjugated CD29, APC conjugated CD31, PerCP-Cy5.5 conjugated CD45, and PE-Cy7 conjugated CD34 were from eBioscience. p63, AR, CK8, CK5, PSA and vimentin antibodies were from Santa Cruz Biotechnology. Testosterone and collagenase IV were from Sigma and human nuclei antibody was from Millipore.
Li W., Ye B., Cai X.Y., Lin J.H, & Gao W.Q. (2014). Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Prostate-Like Epithelial Cells In Vivo. PLoS ONE, 9(7), e102657.
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2

Isolation and Characterization of Wharton's Jelly Cells

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This study was approved by the Navy General Hospital Ethical Committee. With the consent of the parents, human umbilical cord Wharton’s jelly was collected from infants delivered by full-term normal labor. The cells from the Wharton's jelly tissue were isolated as described previously [26 (link)]. Briefly, after removal of blood vessels and epithelium, the Wharton’s jelly was cut into pieces about 1.5–2.5 mm3, and digested for 18 hours at 37 °C with 0.2 mg/ml collagenase (Sigma St. Louis, Missouri, USA) solution in serum-free medium containing 100 U/ml penicillin, 100 mg/ml streptomycin, and 2.5 mg/ml amphotericin B. The isolated cells were suspended in Dulbecco's modified Eagle’s medium (DMEM)/F12 nutrient mixture containing 20 % fetal bovine serum (FBS). When WJCs were cultured to reach 80–90 % confluence, they were passaged and seeded at a density of 4 × 103/cm2 in DMEM/F12 with 10 % FBS. WJCs at passage 3 were taken for flow cytometry analysis as described previously [42 (link)]. The antibodies used were phycoerythrin (PE)-conjugated CD105, CD73, CD45, CD29, CD166, human leukocyte antigen (HLA)-DR, HLA-ABC, and fluorescein isothiocyanate (FITC)-conjugated CD34 and CD90 (all eBioscience, Santiago, California, USA).
Zhang Y., Tao H., Gu T., Zhou M., Jia Z., Jiang G., Chen C., Han Z., Xu C., Wang D., He Q, & Ruan D. (2015). The effects of human Wharton’s jelly cell transplantation on the intervertebral disc in a canine disc degeneration model. Stem Cell Research & Therapy, 6(1), 154.
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3

Quantification of Plasma-Derived Microparticles

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Blood was obtained in sodium citrate and processed within 90 min at room temperature. MP counts were performed from fresh samples across the whole study. Samples were centrifuged for 10 min at 150xg and the supernatant, designated platelet-rich plasma (PRP, was then centrifuged at 13000xg for 2 min to pellet the platelets. The resulting supernatant, designated platelet-poor plasma (PPP) contained MP. A 100μl aliquot of PPP was used to assess MP by flow cytometry and the remainder was centrifuged at 17000xg for 45 min to pellet MP. The MP pellet was re-suspended in modified Tyrode’s buffer containing 0.35% BSA and stored at −70°C. MP were analyzed by flow cytometry [Becton-Dickinson LSRI (with upgraded light scatter detectors present on the machine) BD Biosciences] assessing forward and side scatter, and fluorescence using FITC-conjugated annexin V (BD Biosciences) and antibodies specific for endothelial cells ( PE-conjugated CD105 from eBioscience and PE-conjugated CD144 from BD Biosciences ), platelets (FITC-conjugated CD41 from BD Biosciences), leukocytes (PE-conjugated CD18 from BD Biosciences), neutrophils (PE-conjugated CD16b from BD Biosciences) and monocytes (FITC-conjugated CD14 from BD Biosciences). Each marker was counted separately (see supplementary data for gating strategy).
Hajj-Ali R., Major J., Langford C., Hoffman G., Clark T., Zhang L., Sun Z, & Silverstein R. (2015). The interface of inflammation and subclinical atherosclerosis in Granulomatosis with Polyangiitis (Wegener’s): a preliminary study. Translational research : the journal of laboratory and clinical medicine, 166(4), 366-374.
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4

Immunophenotyping of Menstrual Stem Cells

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P3 MenSCs-DGC (n=5) and MenSCs-RLB (n=5) were used for the immunophenotyping analysis. Mouse anti-human monoclonal antibodies: FITC-conjugated CD29 (Cat#: 11-0299-42, dilution ratio 1:20, eBioscience), CD44 (Cat#: 11-0441-82, dilution ratio 1:20, eBioscience), CD73 (Cat#: 11-0739-42, dilution ratio 1:20, eBioscience), CD90 (Cat#: 11-0909-42, dilution ratio 1:20, eBioscience), HLA-ABC (Cat#: 11-9983-42, dilution ratio 1:20, eBioscience), HLA-DR (Cat#: 11-9956-42, dilution ratio 1:20, eBioscience), CD34 (Cat#: 11-0349-42, dilution ratio 1:20, eBioscience), CD45 (Cat#: 11-9459-42, dilution ratio 1:20, eBioscience) and PE-conjugated CD105 (Cat#: 12-1057-42, dilution ratio 1:20, eBioscience) were used. As negative controls, isotype PE (Cat#: 12-4714-42, dilution ratio 1:20, eBioscience) and FITC-conjugated IgG (Cat#: 31505, dilution ratio 1:20, eBioscience) were used. The cell suspensions (1×106 cells) were washed twice with PBS and incubated with the monoclonal antibodies at 4°C in the dark for 30 min. After washing with PBS, the samples were resuspended and analyzed using a Cytomics FC 500 MPL cytometer (Beckman Coulter).
Sun Y., Ren Y., Yang F., He Y., Liang S., Guan L., Cheng F., Liu Y, & Lin J. (2019). High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment. Biology Open, 8(5), bio038885.
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