Mpeg sh

Manufactured by Merck Group

MPEG-SH is a laboratory equipment product manufactured by Merck Group. It is a type of polyethylene glycol (PEG) that is commonly used in various scientific applications. The core function of MPEG-SH is to serve as a reagent for the modification and stabilization of proteins, enzymes, and other biomolecules.

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Poly(ethylene glycol)methyl ether thiol (mPEG-SH) (2 citations) MPEG-SH 5000 (2 citations)

8 protocols using mpeg sh

Multifunctional Theranostic Nanoparticles

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Materials. All chemicals involved in this work were analytical grade and used without further purification. Sodium alginate, Ethylene glycol, PEI25K, EDC, NHS, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), IR-820 and mPEG-SH were purchased from Sigma-Aldrich and used as received. HAuCl₄, K₂CO₃, and NH₄COOH were purchased from Sinopharm Chemical Reagent Co. Ltd, Shanghai, China. FeCl₃.6H₂O and Doxorubicin chloride was obtained from Zhejiang Hisun Pharmaceutical Company, Zhejiang, China.

Peng J., Qi T., Liao J., Chu B., Yang Q., Qu Y., Li W., Li H., Luo F, & Qian Z. (2014). Mesoporous Magnetic Gold “Nanoclusters” as Theranostic Carrier for Chemo-Photothermal Co-therapy of Breast Cancer. Theranostics, 4(7), 678-692.

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Synthesis and Characterization of Silver Nanoparticles

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To control for size and stability, AgNPs were prepared as recently described with AgClO₄ and an oleic acid coating [14 ,15 (link)]. Briefly, for monodispersed AgNPs, AgClO₄ was reduced by oleylamine at varying temperatures to produce different sizes and coated in situ with oleic acid for stability. These materials were made water soluble by coating with mPEG-SH (Mn = 5000; Sigma) through a ligand exchange reaction. After a phase transfer into water, the mPEG-SH surface coating limited the dissolution of ionic silver.
The size and chemical composition of AgNPs were carefully measured using several characterization tools (Figure 1). The silver core diameter was determined by transmission electron microscopy (Jeol 2010 TEM). Dynamic light scattering (Malvern Zetasizer Nano-ZS) was used to characterize the hydrodynamic diameter and zeta potential of the nanoparticles in solution (Figure 1). The silver ion concentration was determined by inductively coupled plasma–mass spectrometry (ICP-MS). All concentrations were based on milligrams of silver ion in solution. Three AgNP treatments at the sublethal concentrations of 1 mg Ag/L, 10 mg Ag/L, and 100 mg Ag/L were set. All reagents were of analytical grade and were supplied by Sigma Aldrich.

Contreras E.Q., Puppala H.L., Escalera G., Zhong W, & Colvin V.L. (2014). SIZE-DEPENDENT IMPACTS OF SILVER NANOPARTICLES ON THE LIFESPAN, FERTILITY, GROWTH, AND LOCOMOTION OF CAENORHABDITIS ELEGANS. Environmental toxicology and chemistry / SETAC, 33(12), 2716-2723.

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Synthesis of Functional Nanomaterials

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Tetrachloroauric(III) acid trihydrate (HAuCl₄· 3H₂O), L- ascorbic acid (ACS reagent 99%), N-isopropylacrylamide (NIPAM, 97%), N,N’-methylenebis(acrylamide) (BIS, 99%), N,N,N’,N’-tetramethylethylenediamine (TEMED), 5-bromosalicylic acid (technical grade 90%), silver nitrate (AgNO₃, ACS reagent 99.0%), sodium borohydride (NaBH₄, ReagentPlus 99%), O-[2-(3-mercaptopropionylamino)ethyl]-O’-methyl-poly(ethylene glycol) (mPEG-SH, Mw 5000), trisodium citrate dihydrate, titanium tetrachloride (TiCl₄), (3-aminopropyl)trimethoxysilane (APS), Pluronic F127 (HO(CH₂CH₂O)₁₀₆(CH₂CH(CH₃)-O)₇₀(CH₂CH₂O)₁₀₆OH), hydrogen peroxide (H₂O₂, 28%), sulfuric acid (H₂SO₄, 98%), chloroform (99.8%) and pyocyanin from Pseudomonas aeruginosa were supplied by Sigma Aldrich. Hydrochloric acid (HCl, 37 wt % in water) was supplied by Panreac. Cetyltrimethylammonium bromide (CTAB, 96%) was supplied by Fluka. All chemicals were used as received. Pure grade ethanol and Milli-Q water were used as solvents.

Bodelón G., Montes-García V., López-Puente V., Hill E.H., Hamon C., Sanz-Ortiz M.N., Rodal-Cedeira S., Costas C., Celiksoy S., Pérez-Juste I., Scarabelli L., Porta A.L., Pérez-Juste J., Pastoriza-Santos I, & Liz-Marzán L.M. (2016). Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering. Nature materials, 15(11), 1203-1211.

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Mesoporous Silica-Coated Gold Nanorods

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A mesoporous silica coating on GNR was achieved by the Gorelikov and Matsuura protocol [26 (link)]. Two hundred microliters (200 μL) of 0.1 m NaOH solution was added to 30 mL of the as-prepared GNR solution. Then, 20 μL of 20% tetraethylorthosilicate (TEOS) in methanol solvent was injected three times in 30 min intervals under gentle stirring. Growth of the mesoporous silica layer was allowed to continue with vigorous stirring at 600 rpm for 3 days at 30 °C. The remaining excess reagents in the resulting product were removed by centrifugation and washing three times with methanol. The purified GNR@SiO₂ was dispersed in DI water and then PEGy-lated by using poly(ethylene glycol) methyl ether thiol (mPEG-SH, MW: 1000, Sigma–Aldrich) to enhance the stability. The resulting GNR@SiO₂ solution was mixed with excess MB (0.2 g) under stirring for 3 days at room temperature. In order to completely eliminate the physically adsorbed MB molecules, the MB-loaded GNR@SiO₂ solution was repeatedly centrifuged and washed with DI water until the supernatant solution changed color from dark blue to colorless. From the ICP-OES analysis, Au concentration of the MB-GNR@SiO₂ solution was determined to be 94.8 μg Au/mL.

Seo S.H., Kim B.M., Joe A., Han H.W., Chen X., Cheng Z, & Jang E.S. (2014). NIR-light-induced surface-enhanced Raman scattering for detection and photothermal/photodynamic therapy of cancer cells using methylene blue-embedded gold nanorod@SiO2 nanocomposites. Biomaterials, 35(10), 3309-3318.

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Synthesis and Functionalization of Immunogenic Adenovirus Nanoparticles

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Reagents required for the preparation of the iAuNPs, including trisodium citrate, sodium borohydride, ascorbic acid, and CTAB, were purchased from Sigma-Aldrich. Hydrogen tetrachloroaurate (III) hydrate was obtained from Acros Organics. Poly(ethylene glycols) employed for the PEGylation (HS-PEG-NH₂ with average Mn 2000 and mPEG-SH with average Mn 800) and glutaraldehyde were purchased from Sigma-Aldrich. Purified hexon protein from adenovirus type 5 (Adv5) (purified adenovirus type 5 hexon-liquid prepared in 10 mM bis-tris propane buffer, 375 mM NaCl at a concentration of 0.39 mg mL⁻¹) was supplied by Bio-Rad. Protein LoBind tubes of 1.5, 2, and 15 mL from VWR were used for all experiments.

Fresco-Cala B., López-Lorente Á.I., Batista A.D., Dinc M., Bansmann J., Behm R.J., Cárdenas S, & Mizaikoff B. (2022). Icosahedral gold nanoparticles decorated with hexon protein: a surrogate for adenovirus serotype 5. Analytical and Bioanalytical Chemistry, 415(11), 2081-2090.

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Immunoassay Development Protocol

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Sodium tetrachloropalladate(II) (Na₂PdCl₄, 98%), potassium bromide (KBr, ≥ 99%), polyvinylpyrrolidone (PVP, M.W. ≈ 55,000), L-ascorbic acid (AA, ≥ 99%), sodium borohydride (NaBH₄, 98%), sodium hexachloroiridate(III) hydrate (Na₃IrCl₆ • xH₂O, M.W. = 473.9), acetic acid (HOAc, ≥ 99.7%), sodium acetate (NaOAc, ≥ 99%), 3,3′,5,5′-tetramethylbenzidine (TMB, > 99%), hydrogen peroxide solution (30% w/v in H₂O), sulfuric acid (H₂SO₄, 95–98%), dimethylformamide (DMF), potassium phosphate monobasic (KH₂PO₄, ≥ 99%), sodium phosphate dibasic (Na₂HPO₄, ≥ 99%), potassium chloride (KCl, ≥ 99%), sodium chloride (NaCl, ≥ 99.5%), hydrochloric acid (HCl, 37%), bovine serum albumin (BSA, ≥ 98%), Tween 20, mPEG-SH (5 kDa), and human prostate-specific antigen (PSA, ≥ 99%) were all received from Sigma-Aldrich. Goat anti-mouse IgG, HRP-Goat anti-mouse IgG conjugates, rabbit anti-NP monoclonal antibody (mAb), and mouse anti-NP mAb were obtained from Thermo Fisher Scientific, Inc. Mouse anti-PSA mAb and rabbit anti-PSA polyclonal antibody (pAb) were obtained from Abcam plc. Pooled human saliva was bought from Innovative Research.

Li J, & Li Y. (2021). One-pot high-yield synthesis of Pd nanocubes for Pd-Ir nanocube-based immunoassay of nucleocapsid protein from SARS-CoV-2. Analytical and Bioanalytical Chemistry, 413(18), 4635-4644.

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Synthesis of Functional Nanomaterials

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Synthesis of PEGylated Gold Nanoparticles

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Monodispersed gold nanoparticles were prepared according to literature procedures19 (link) with slight modifications. In brief, a 100 mL aqueous solution of HAuCl₄ 4H₂O (1 mM, 99.99%, Sigma) was brought to the boil with vigorous stirring, and then 10 mL of sodium citrate tribasic dihydrate solution (38.8 mM) was quickly added. After the solution color changed to deep red, the solution was refluxed for an additional 15 min. Ten milligrams of poly(ethylene glycol) methyl ether thiol (mPEG-SH) (M_W=6000, Sigma) was added to 10 mL of above aqueous solution, and stirred for 24 h to complete the ligand exchange (citrate to PEG).

Sukkar F., Shafaa M., El-Nagdy M, & Darwish W. (2021). Polymeric Nanocarriers for Effective Synergistic Action of Sorafenib Tosylate and Gold-sensitized Gamma Radiation Against HepG2 Cells. International Journal of Nanomedicine, 16, 8309-8321.

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