RIP assay (Millipore, Billerica, MA) was carried out according to the manufacturer’s instructions. The RIP lysate was obtained and centrifuged at 12,000 rpm for 10 minutes. Add 100 µL of the supernatant to the RIP immunoprecipitation buffer containing the magnetic bead-antibody complex; then, the RNA was purified and obtained from TRIzol. Finally, the immunoprecipitated RNA was analyzed by RT-PCR.
Rip assay
Manufactured by Merck Group
The RIP assay is a laboratory tool used to detect and quantify protein-RNA interactions. It involves the immunoprecipitation of a target RNA-binding protein, followed by the identification and analysis of associated RNA molecules. The RIP assay provides researchers with a means to study the complex regulatory networks involving RNA-protein interactions, which are crucial for various cellular processes.
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Lab products found in correlation
3 protocols using rip assay
1
RNA Immunoprecipitation Assay
2
RIP Assay for RNA Isolation
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The RIP lysate was obtained and centrifuged at 12,000 rpm for 10 minutes. RIP assay (Millipore, Billerica, MA) was carried out according to the manufacturer's instructions. Cells were fixed by 1% formaldehyde, lysed, sonicated, and incubated overnight; on the second day, the samples were incubated with 200 ml lysis buffer and protease K to reverse formaldehyde crosslinking. The RNA was purified and obtained from TRIzol. Finally, the immunoprecipitated RNA was detected by RT-PCR.
3
RIP Assay for RNA Immunoprecipitation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RIP assay (Millipore, Billerica, MA) was carried out according to the manufacturer's instructions. The RIP lysate was obtained and centrifuged at 14,000 rpm for 10 minutes. Add 100 µL of the supernatant to the RIP immunoprecipitation buffer containing the magnetic bead-antibody complex, then the RNA was puri ed and obtained from TRIzol. Finally, analysis of immunoprecipitated RNA by RT-PCR.
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