U44 snrna

Erk1/2 (T202/Y204), Akt (S473), Fak (397Tyr) phosphor-antibodies and Erk1/2, Akt, Blimp-1/PRDI-BF1 and cleaved caspase-3 antibodies were from Cell Signaling. Vinculin antibody was produced in our laboratory. Fak polyclonal antibodies were from Abcam. GAPDH antibodies from Millipore, p130Cas antibodies were from BD Biosciences. Matrigel and collagen bovine I were from Corning. Peroxidase-conjugated secondary antibodies were from GE Healthcare. Pre-miR™ microRNA Precursor Molecules for Negative Control #1, Hsa-miR-23b (PM10711), anti-miR™ miRNA Inhibitor Negative Control #1, anti-miR™ miRNA Inhibitor Hsa-miR-23b-3p (AM10711) (Ambion) and TaqMan® MicroRNA Assays for Hsa-miR-23b (ID 000399), U44 snRNA (ID 001094) were from Applied Biosystems.

miR precursors: Pre-miR™ miRNA Precursor Negative Control #1, Pre-miR™ miRNA Precursor hsa-miR-148b (PM10264) (Applied Biosystems). TaqMan® MicroRNA assays for miRNA detection: Hsa-miR-148b-3p ID 000471, U6 snRNA ID001973, U44 snRNA ID001904 (Applied Biosystems). Quantitect Primer Assay: 218300Axl ID 33000 (Qiagen). Qiagen miScript-SYBR Green PCR Kit and miScript Primer Assay: hsa-let-7g ID 1 (Qiagen). Primary antibodies: anti-Cleaved Caspase-3 (Asp175) #9661 (Cell Signaling Technology), anti-Ki67 ab15580 (Abcam), anti-ITGA5 pAb RM10 kindly provided by G. Tarone laboratory (Molecular Biotechnology Center, University of Torino), anti-CD166/ALCAM mAb MOG/07 (Novocastra Laboratories), anti-GAPDH pAb V-18, anti-ACTIN I-19 pAb (all form Santa Cruz Biotechnology), anti-AXL (R&D Systems). Secondary antibodies: HRP-conjugated goat anti-mouse IgG, goat anti-rabbit IgG (Santa Cruz Biotechnology).