Salivary glands and spleens were removed at the indicated ages. Tissues were frozen in OCT, and blocks were stored at −70°C until sectioning. Frozen tissues were sectioned (5 μm thickness), fixed in acetone, and stained with the following primary Abs: anti-IgM–Texas Red (SouthernBiotech), anti-B220, anti-CD4–FITC, anti-CD8–FITC, anti-GL7–FITC, anti–peanut agglutinin (PNA)–FITC, and biotinylated PNA (Vector Laboratories, Burlingame, CA). Anti-rat–Oregon Green and streptavidin–Oregon Green were used as secondary Abs. All Abs were purchased from Life Technologies, unless otherwise indicated. The number of splenic GCs was determined by dividing the total number of GCs by the total number of B cell follicles, to normalize for area in each cryosection. Two or three cryosections per mouse were evaluated. Expression of CD3 and B220 in thyroids and salivary glands was examined using formaldehyde-fixed paraffin sections, as previously described (8 (link)). All fixed tissue was stained by IDEXX RADIL (Columbia, MO).
Anti cd8 fitc
Manufactured by Vector Laboratories
Anti-CD8–FITC is a fluorochrome-conjugated antibody that specifically binds to the CD8 antigen expressed on the surface of certain T cells. It is designed for use in flow cytometry and other immunoassay applications to identify and quantify CD8-positive cells in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Anti igm texas red, by Southern Biotech (2 mentions)
Anti b220, by Southern Biotech (2 mentions)
Anti cd4 fitc, by Vector Laboratories (2 mentions)
Anti gl7 fitc, by Vector Laboratories (2 mentions)
Anti peanut agglutinin pna fitc, by Vector Laboratories (2 mentions)
Biotinylated pna, by Vector Laboratories (2 mentions)
2 protocols using anti cd8 fitc
1
Tissue Immunohistochemistry Analysis
2
Tissue Immunohistochemistry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Salivary glands and spleens were removed at the indicated ages. Tissues were frozen in OCT, and blocks were stored at −70°C until sectioning. Frozen tissues were sectioned (5 μm thickness), fixed in acetone, and stained with the following primary Abs: anti-IgM–Texas Red (SouthernBiotech), anti-B220, anti-CD4–FITC, anti-CD8–FITC, anti-GL7–FITC, anti–peanut agglutinin (PNA)–FITC, and biotinylated PNA (Vector Laboratories, Burlingame, CA). Anti-rat–Oregon Green and streptavidin–Oregon Green were used as secondary Abs. All Abs were purchased from Life Technologies, unless otherwise indicated. The number of splenic GCs was determined by dividing the total number of GCs by the total number of B cell follicles, to normalize for area in each cryosection. Two or three cryosections per mouse were evaluated. Expression of CD3 and B220 in thyroids and salivary glands was examined using formaldehyde-fixed paraffin sections, as previously described (8 (link)). All fixed tissue was stained by IDEXX RADIL (Columbia, MO).
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