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Radiance 2100 rainbow

Manufactured by Nikon
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The Radiance 2100 Rainbow is a versatile lab equipment product designed for various scientific applications. It functions as a spectrophotometer, capable of measuring the absorbance or transmittance of light across a range of wavelengths. The device provides accurate and reliable data for researchers and scientists in their laboratory work.

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Lab products found in correlation

Illustrator cs3, by Adobe (2 mentions) Zen software, by Zeiss (2 mentions) Lasersharp 2000, by Bio-Rad (2 mentions) Velocity software, by PerkinElmer (2 mentions) Nis elements software, by Nikon (2 mentions) E800 microscope, by Nikon (1 mentions)

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Radiance 2100 (3 citations)

3 protocols using radiance 2100 rainbow

1

Multimodal Fluorescence Microscopy Imaging

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Images were captured on a fluorescence microscope (Nikon E90i or Nikon E1000) equipped with a Nikon Intensilight C-HGFI source or an X-Cite 120 illuminator (EXFO), respectively, using high numerical aperture optics and a cooled CCD camera (ANDOR or Q-Imaging, respectively) running NIS Elements software (Nikon) or Velocity software (Improvision, Lexington, MA).
Five-channel confocal imaging was performed at the Harvard Center for Biological Imaging on a Zeiss 710 inverted confocal microscope equipped for spectral unmixing and for acquisition of confocal montages of entire tissue sections, running ZEN software (Zeiss). Other confocal images were obtained using a BioRad Radiance 2100 Rainbow laser-scanning confocal system based on a Nikon E800 microscope with LaserSharp 2000 imaging software (Bio-Rad Laboratories). Some images were subsequently processed for 3D reconstruction in Imaris (Bitplane). For an optimal visual reproduction of the data, images were adjusted for contrast, brightness, color balance, and size in Photoshop CS3 (Adobe, San Jose, CA), and assembled in Power Point for Mac 2011 (Microsoft Corporation, Redmond, WA), and in Illustrator CS3 (Adobe, San Jose, CA).
Wuttke T.V., Markopoulos F., Padmanabhan H., Wheeler A.P., Murthy V.N, & Macklis J.D. (2018). Developmentally primed cortical neurons maintain fidelity of differentiation and establish appropriate functional connectivity after transplantation. Nature neuroscience, 21(4), 517-529.
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2

Fluorescence Microscopy Imaging Protocol

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Cells were fixed in 4% paraformaldehyde (wt/vol) for 30 minutes, and washed three times in phosphate-buffered saline (PBS). Widefield image acquisition was performed with a Nikon 90i epifluorescence microscope with a Clara DR-328G cooled CCD digital camera (Andor Technology, Belfast, Northern Ireland). Confocal imaging was performed with a Bio-Rad Radiance 2100 Rainbow laser scanning confocal microscope based on a Nikon E800 microscope. Images were assembled in Adobe Photoshop and Illustrator (CS3, CS5), with adjustments for contrast, brightness, and color balance to obtain optimal visual reproduction of data.
Sadegh C, & Macklis J.D. (2014). Established Monolayer Differentiation of Mouse Embryonic Stem Cells Generates Heterogeneous Neocortical-Like Neurons Stalled at a Stage Equivalent to Midcorticogenesis. The Journal of comparative neurology, 522(12), 2691-2706.
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3

Multimodal Fluorescence Microscopy Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were captured on a fluorescence microscope (Nikon E90i or Nikon E1000) equipped with a Nikon Intensilight C-HGFI source or an X-Cite 120 illuminator (EXFO), respectively, using high numerical aperture optics and a cooled CCD camera (ANDOR or Q-Imaging, respectively) running NIS Elements software (Nikon) or Velocity software (Improvision, Lexington, MA).
Five-channel confocal imaging was performed at the Harvard Center for Biological Imaging on a Zeiss 710 inverted confocal microscope equipped for spectral unmixing and for acquisition of confocal montages of entire tissue sections, running ZEN software (Zeiss). Other confocal images were obtained using a BioRad Radiance 2100 Rainbow laser-scanning confocal system based on a Nikon E800 microscope with LaserSharp 2000 imaging software (Bio-Rad Laboratories). Some images were subsequently processed for 3D reconstruction in Imaris (Bitplane). For an optimal visual reproduction of the data, images were adjusted for contrast, brightness, color balance, and size in Photoshop CS3 (Adobe, San Jose, CA), and assembled in Power Point for Mac 2011 (Microsoft Corporation, Redmond, WA), and in Illustrator CS3 (Adobe, San Jose, CA).
Wuttke T.V., Markopoulos F., Padmanabhan H., Wheeler A.P., Murthy V.N, & Macklis J.D. (2018). Developmentally primed cortical neurons maintain fidelity of differentiation and establish appropriate functional connectivity after transplantation. Nature neuroscience, 21(4), 517-529.
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