Apc anti human mouse cd49f

For FACS or flow experiments, single-cell suspensions of cells were resuspended in a 2% FBS solution and then stained with appropriate antibodies at a 1:100 dilution for 60 min at 4 °C in the dark. Antibodies used were FITC anti-mouse Ly6d (BioLegend; 138606) and APC anti-human/mouse Cd49f (BioLegend; 313616). Before sorting or flow, SytoxBlue (Thermo Fisher; S34857) was used for a viability dye at a 1:1000 dilution. FACS experiments were run on FACSAria II, and flow experiments were run on LSRII instruments. Both instruments used the BD FACSDiva 8.0.1 software for data collection. For scRNA-Seq experiments, cells were only stained with SytoxBlue, and live cells were sorted for downstream processing and experimentation. For EdU experiments, we used the Click-iT Plus EdU Alexa Flour 647 Flow Cytometry Assay Kit as per manufacturer instructions (Invitrogen; C10634). For Apotracker^TM experiments, we used the Apotracker^TM Green reagent as per manufacturer instructions (BioLegend; 427402). FlowJo 10.6 was used for data analysis.