Log In Sign up
The largest database of trusted experimental protocols

Mouse anti enos monoclonal antibody

Manufactured by BD
Visit Supplier

Mouse anti-eNOS monoclonal antibody is a laboratory reagent used for the detection and quantification of endothelial nitric oxide synthase (eNOS) in various biological samples. This antibody binds specifically to eNOS, a critical enzyme involved in the production of nitric oxide, which plays a vital role in regulating vascular function and homeostasis.

Automatically generated - may contain errors

Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Miniature slab gel apparatus, by Bio-Rad (1 mentions) Rabbit anti phospho ampk thr172 antibody, by Cell Signaling Technology (1 mentions) Rabbit anti ampk antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-eNOS (49 citations) Mouse anti-eNOS (14 citations) Anti-eNOS antibody (12 citations) ENOS antibody (5 citations) Mouse monoclonal anti-eNOS (3 citations) Mouse anti (2 citations)

2 protocols using mouse anti enos monoclonal antibody

1

Western Blot Analysis of Protein Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The samples were prepared as described previously [33 (link)]. Protein concentrations were quantified by the BCA Protein Assay Kit (Thermo, Rockford, USA). Samples containing equal amounts of protein were subjected to SDS-PAGE in a Bio-Rad miniature slab gel apparatus and electrophoretically transferred onto a nitrocellulose membrane. The membranes were incubated with 5 % BSA/PBST for 1 h at 25 °C, and incubated overnight at 4 °C with primary antibodies and 1 h at 25 °C with secondary antibodies respectively. Cell Signaling Technology Inc (Danvers, Mass) was the supplier for the following primary antibodies: rabbit polyclonal anti-phospho-eNOS (Ser1177) antibody, rabbit anti-phospho-AMPK (Thr172) antibody, rabbit anti-AMPK antibody. Mouse anti-eNOS monoclonal antibody was obtained from BD Transduction Laboratories (Lexington, Ky).
Yu J.W., Deng Y.P., Han X., Ren G.F., Cai J, & Jiang G.J. (2016). Metformin improves the angiogenic functions of endothelial progenitor cells via activating AMPK/eNOS pathway in diabetic mice. Cardiovascular Diabetology, 15, 88.
+ Open protocol
+ Expand
2

eNOS Homodimer Formation in Myocardium

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The LV was harvested and homogenized, and immunoblots were performed using standard techniques, as described.25 (link) The normal function of eNOS requires dimerization of the enzyme.26 (link) To investigate eNOS homodimer formation in the myocardium, non-boiled cellular lysate was resolved by 6% SDS-PAGE at 4°C overnight. Membranes were incubated with a mouse anti-eNOS monoclonal antibody (BD Transduction Laboratories).
Baumgardt S.L., Paterson M., Leucker T.M., Fang J., Zhang D.X., Bosnjak Z.J., Warltier D.C., Kersten J.R, & Ge Z.D. (2016). Chronic Co-Administration of Sepiapterin and L-Citrulline Ameliorates Diabetic Cardiomyopathy and Myocardial Ischemia/Reperfusion Injury in Obese Type 2 Diabetic Mice. Circulation. Heart failure, 9(1), e002424.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!